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- W4220932765 endingPage "110476" @default.
- W4220932765 startingPage "110476" @default.
- W4220932765 abstract "Targeted delivery of therapeutic proteins toward specific cells and across cell membranes remains major challenges. Here, we develop protein-based delivery systems utilizing detoxified single-chain bacterial toxins such as diphtheria toxin (DT) and botulinum neurotoxin (BoNT)-like toxin, BoNT/X, as carriers. The system can deliver large protein cargoes including Cas13a, CasRx, Cas9, and Cre recombinase into cells in a receptor-dependent manner, although delivery of ribonucleoproteins containing guide RNAs is not successful. Delivery of Cas13a and CasRx, together with guide RNA expression, reduces mRNAs encoding GFP, SARS-CoV-2 fragments, and endogenous proteins PPIB, KRAS, and CXCR4 in multiple cell lines. Delivery of Cre recombinase modifies the reporter loci in cells. Delivery of Cas9, together with guide RNA expression, generates mutations at the targeted genomic sites in cell lines and induced pluripotent stem cell (iPSC)-derived human neurons. These findings establish modular delivery systems based on single-chain bacterial toxins for delivery of membrane-impermeable therapeutics into targeted cells." @default.
- W4220932765 created "2022-04-03" @default.
- W4220932765 creator A5009988205 @default.
- W4220932765 creator A5015133641 @default.
- W4220932765 creator A5016769340 @default.
- W4220932765 creator A5055290962 @default.
- W4220932765 creator A5080357960 @default.
- W4220932765 creator A5086559528 @default.
- W4220932765 date "2022-03-01" @default.
- W4220932765 modified "2023-10-12" @default.
- W4220932765 title "Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms" @default.
- W4220932765 cites W1219966704 @default.
- W4220932765 cites W1486349850 @default.
- W4220932765 cites W1531000409 @default.
- W4220932765 cites W1973260880 @default.
- W4220932765 cites W1977993125 @default.
- W4220932765 cites W1982873976 @default.
- W4220932765 cites W1992650953 @default.
- W4220932765 cites W1992950210 @default.
- W4220932765 cites W1993223505 @default.
- W4220932765 cites W2000286590 @default.
- W4220932765 cites W2002846971 @default.
- W4220932765 cites W2003171404 @default.
- W4220932765 cites W2021710989 @default.
- W4220932765 cites W2027103767 @default.
- W4220932765 cites W2040027262 @default.
- W4220932765 cites W2045435533 @default.
- W4220932765 cites W2064815984 @default.
- W4220932765 cites W2068629712 @default.
- W4220932765 cites W2070614858 @default.
- W4220932765 cites W2073217307 @default.
- W4220932765 cites W2073308879 @default.
- W4220932765 cites W2075181546 @default.
- W4220932765 cites W2082661031 @default.
- W4220932765 cites W2089929852 @default.
- W4220932765 cites W2100305420 @default.
- W4220932765 cites W2103609218 @default.
- W4220932765 cites W2105593801 @default.
- W4220932765 cites W2107277218 @default.
- W4220932765 cites W2109257525 @default.
- W4220932765 cites W2111998971 @default.
- W4220932765 cites W2113310136 @default.
- W4220932765 cites W2117419620 @default.
- W4220932765 cites W2120626980 @default.
- W4220932765 cites W2129920068 @default.
- W4220932765 cites W2140293166 @default.
- W4220932765 cites W2146615689 @default.
- W4220932765 cites W2153738223 @default.
- W4220932765 cites W2154288610 @default.
- W4220932765 cites W2169244146 @default.
- W4220932765 cites W2169274230 @default.
- W4220932765 cites W2192080449 @default.
- W4220932765 cites W2264394231 @default.
- W4220932765 cites W2328702355 @default.
- W4220932765 cites W2409671300 @default.
- W4220932765 cites W2419551149 @default.
- W4220932765 cites W2522085729 @default.
- W4220932765 cites W2554763217 @default.
- W4220932765 cites W2565920990 @default.
- W4220932765 cites W2589583594 @default.
- W4220932765 cites W2598060151 @default.
- W4220932765 cites W2605563559 @default.
- W4220932765 cites W2606915822 @default.
- W4220932765 cites W2610767416 @default.
- W4220932765 cites W2623277795 @default.
- W4220932765 cites W2740139987 @default.
- W4220932765 cites W2754892676 @default.
- W4220932765 cites W2763042654 @default.
- W4220932765 cites W2765351619 @default.
- W4220932765 cites W2785353872 @default.
- W4220932765 cites W2793349369 @default.
- W4220932765 cites W2793359586 @default.
- W4220932765 cites W2801459452 @default.
- W4220932765 cites W2804566227 @default.
- W4220932765 cites W2886121055 @default.
- W4220932765 cites W2888908334 @default.
- W4220932765 cites W2903612881 @default.
- W4220932765 cites W2941116679 @default.
- W4220932765 cites W2945691418 @default.
- W4220932765 cites W2949380898 @default.
- W4220932765 cites W2958848569 @default.
- W4220932765 cites W2974708087 @default.
- W4220932765 cites W2976425815 @default.
- W4220932765 cites W2979487934 @default.
- W4220932765 cites W2991116009 @default.
- W4220932765 cites W2992953990 @default.
- W4220932765 cites W3006116138 @default.
- W4220932765 cites W3015141182 @default.
- W4220932765 cites W3016048690 @default.
- W4220932765 cites W3020077371 @default.
- W4220932765 cites W3020862607 @default.
- W4220932765 cites W3030089399 @default.
- W4220932765 cites W3039146726 @default.
- W4220932765 cites W3090758663 @default.
- W4220932765 cites W3118568467 @default.
- W4220932765 cites W3120061304 @default.
- W4220932765 cites W3128627243 @default.
- W4220932765 doi "https://doi.org/10.1016/j.celrep.2022.110476" @default.