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• W4220951618 startingPage "580" @default.
• W4220951618 abstract "Despite a long history of genetic engineering in Carica papaya L., genome editing protocols have not been previously validated for this species. To address this gap in the papaya improvement toolkit, we deployed CRIPSR/Cas9 to target the candidate Carica papaya L. phytoene desaturase (CpPDS) gene with the goal of inducing a visually scorable albino phenotype in edited tissue. A total of 73 plant lines stably transformed with pAC0025 (CRISPR construct targeting CpPDS) were obtained. Of these, 59 (81%) were fully albino. Genotyping of the targeted region within CpPDS was carried out for ten pAC0025 (CpPDS construct) lines, one untransformed control, and one line transformed with a negative control construct pAC0026 (no gRNA construct). Alignment of Sanger sequencing results revealed diverse edits in pAC0025-transformed plants including insertions of up to 415 bp, inversions and large deletions between gRNA target sites, and many smaller deletions. Mutations were observed at all three gRNA target sites. Overall, the high percentage of albino plants recovered in combination with the abundance of mutations observed across CpPDS gRNA target sites suggests an efficient genome editing protocol that could be used to improve papaya at the genetic level." @default.
• W4220951618 created "2022-04-03" @default.
• W4220951618 creator A5006785110 @default.
• W4220951618 creator A5062083395 @default.
• W4220951618 date "2022-03-06" @default.
• W4220951618 modified "2023-10-02" @default.
• W4220951618 title "CRISPR/Cas9-mediated genome editing of <i>phytoene desaturase</i> in <i>Carica papaya</i> L" @default.
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• W4220951618 doi "https://doi.org/10.1080/14620316.2022.2038699" @default.
• W4220951618 hasPublicationYear "2022" @default.
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