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• W4220953446 abstract "The Methyloprofundus clade is represented by uncultivated methanotrophic bacterial endosymbionts of deep-sea bathymodiolin mussels, but only a single free-living species has been cultivated to date. This study reveals the existence of free-living Methyloprofundus variants in the Iheya North deep-sea hydrothermal field in the mid-Okinawa Trough. A clade-targeted amplicon analysis of the particulate methane monooxygenase gene (pmoA) detected 647 amplicon sequence variants (ASVs) of the Methyloprofundus clade in microbial communities newly formed in in situ colonization systems. Such systems were deployed at colonies of bathymodiolin mussels and a galatheoid crab in diffuse-flow areas. These ASVs were classified into 161 species-like groups. The proportion of the species-like groups representing endosymbionts of mussels was unexpectedly low. A methanotrophic bacterium designated INp10, a likely dominant species in the Methyloprofundus population in this field, was enriched in a biofilm formed in a methane-fed cultivation system operated at 10°C. Genomic characterization with the gene transcription data set of INp10 from the biofilm suggested traits advantageous to niche competition in environments, such as mobility, chemotaxis, biofilm formation, offensive and defensive systems, and hypoxia tolerance. The notable metabolic traits that INp10 shares with some Methyloprofundus members are the use of lanthanide-dependent XoxF as the sole methanol dehydrogenase due to the absence of the canonical MxaFI, the glycolytic pathway using fructose-6-phosphate aldolase instead of fructose-1,6-bisphosphate aldolase, and the potential to perform partial denitrification from nitrate under oxygen-limited conditions. These findings help us better understand the ecological strategies of this possibly widespread marine-specific methanotrophic clade. IMPORTANCE The Iheya North deep-sea hydrothermal field in the mid-Okinawa Trough is characterized by abundant methane derived from organic-rich sediments and diverse chemosynthetic animal species, including those harboring methanotrophic bacterial symbionts, such as bathymodiolin mussels Bathymodiolus japonicus and Bathymodiolus platifrons and a galatheoid crab, Shinkaia crosnieri. Symbiotic methanotrophs have attracted significant attention, and yet free-living methanotrophs in this environment have not been studied in detail. We focused on the free-living Methyloprofundus spp. that thrive in this hydrothermal field and identified an unexpectedly large number of species-like groups in this clade. Moreover, we enriched and characterized a methanotroph whose genome sequence indicated that it corresponds to a new species in the genus Methyloprofundus. This species might be a dominant member of the indigenous Methyloprofundus population. New information on free-living Methyloprofundus populations suggests that the hydrothermal field is a promising locale at which to investigate the adaptive capacity and associated genetic diversity of Methyloprofundus spp." @default.
• W4220953446 created "2022-04-03" @default.
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• W4220953446 date "2022-01-25" @default.
• W4220953446 modified "2023-10-14" @default.
• W4220953446 title "Multispecies Populations of Methanotrophic <i>Methyloprofundus</i> and Cultivation of a Likely Dominant Species from the Iheya North Deep-Sea Hydrothermal Field" @default.
• W4220953446 cites W1529816837 @default.
• W4220953446 cites W1577051069 @default.
• W4220953446 cites W1701869895 @default.
• W4220953446 cites W1728555672 @default.
• W4220953446 cites W1765345420 @default.
• W4220953446 cites W1767480828 @default.
• W4220953446 cites W1782601010 @default.
• W4220953446 cites W1788146458 @default.
• W4220953446 cites W1816481481 @default.
• W4220953446 cites W1876564902 @default.
• W4220953446 cites W1894306620 @default.
• W4220953446 cites W1919814215 @default.
• W4220953446 cites W1933115513 @default.
• W4220953446 cites W1944015428 @default.
• W4220953446 cites W1957272202 @default.
• W4220953446 cites W1966209795 @default.
• W4220953446 cites W1967351922 @default.
• W4220953446 cites W1974028185 @default.
• W4220953446 cites W1978744189 @default.
• W4220953446 cites W1979081699 @default.
• W4220953446 cites W1982855075 @default.
• W4220953446 cites W1983403167 @default.
• W4220953446 cites W1985319478 @default.
• W4220953446 cites W1985675685 @default.
• W4220953446 cites W1989871058 @default.
• W4220953446 cites W1990148665 @default.
• W4220953446 cites W1992751974 @default.
• W4220953446 cites W1993695323 @default.
• W4220953446 cites W1996835973 @default.
• W4220953446 cites W1999927599 @default.
• W4220953446 cites W2013796530 @default.
• W4220953446 cites W2015402183 @default.
• W4220953446 cites W2017739313 @default.
• W4220953446 cites W2017757481 @default.
• W4220953446 cites W2020367313 @default.
• W4220953446 cites W2023739096 @default.
• W4220953446 cites W2026611778 @default.
• W4220953446 cites W2029408958 @default.
• W4220953446 cites W2034285706 @default.
• W4220953446 cites W2035612098 @default.
• W4220953446 cites W2035775871 @default.
• W4220953446 cites W2036897871 @default.
• W4220953446 cites W2037518717 @default.
• W4220953446 cites W2041446253 @default.
• W4220953446 cites W2048673910 @default.
• W4220953446 cites W2051425632 @default.
• W4220953446 cites W2056279562 @default.
• W4220953446 cites W2056398498 @default.
• W4220953446 cites W2060083881 @default.
• W4220953446 cites W2060095084 @default.
• W4220953446 cites W2060683845 @default.
• W4220953446 cites W2060757154 @default.
• W4220953446 cites W2062941070 @default.
• W4220953446 cites W2063625511 @default.
• W4220953446 cites W2065231890 @default.
• W4220953446 cites W2080256151 @default.
• W4220953446 cites W2080283023 @default.
• W4220953446 cites W2082746601 @default.
• W4220953446 cites W2083880740 @default.
• W4220953446 cites W2085295198 @default.
• W4220953446 cites W2085656216 @default.
• W4220953446 cites W2086554780 @default.
• W4220953446 cites W2088966874 @default.
• W4220953446 cites W2091547311 @default.
• W4220953446 cites W2091671275 @default.
• W4220953446 cites W2091922579 @default.
• W4220953446 cites W2096038227 @default.
• W4220953446 cites W2096297162 @default.
• W4220953446 cites W2097567078 @default.
• W4220953446 cites W2098240442 @default.
• W4220953446 cites W2108350820 @default.
• W4220953446 cites W2110067433 @default.
• W4220953446 cites W2111843956 @default.
• W4220953446 cites W2111873973 @default.
• W4220953446 cites W2112364185 @default.
• W4220953446 cites W2112880344 @default.
• W4220953446 cites W2114956488 @default.
• W4220953446 cites W2115888213 @default.
• W4220953446 cites W2116449689 @default.
• W4220953446 cites W2119888547 @default.

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