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- W4220958770 endingPage "e1009977" @default.
- W4220958770 startingPage "e1009977" @default.
- W4220958770 abstract "Plasmodium falciparum exports ~10% of its proteome into its host erythrocyte to modify the host cell's physiology. The Plasmodium export element (PEXEL) motif contained within the N-terminus of most exported proteins directs the trafficking of those proteins into the erythrocyte. To reach the host cell, the PEXEL motif of exported proteins is processed by the endoplasmic reticulum (ER) resident aspartyl protease plasmepsin V. Then, following secretion into the parasite-encasing parasitophorous vacuole, the mature exported protein must be unfolded and translocated across the parasitophorous vacuole membrane by the Plasmodium translocon of exported proteins (PTEX). PTEX is a protein-conducting channel consisting of the pore-forming protein EXP2, the protein unfoldase HSP101, and structural component PTEX150. The mechanism of how exported proteins are specifically trafficked from the parasite's ER following PEXEL cleavage to PTEX complexes on the parasitophorous vacuole membrane is currently not understood. Here, we present evidence that EXP2 and PTEX150 form a stable subcomplex that facilitates HSP101 docking. We also demonstrate that HSP101 localises both within the parasitophorous vacuole and within the parasite's ER throughout the ring and trophozoite stage of the parasite, coinciding with the timeframe of protein export. Interestingly, we found that HSP101 can form specific interactions with model PEXEL proteins in the parasite's ER, irrespective of their PEXEL processing status. Collectively, our data suggest that HSP101 recognises and chaperones PEXEL proteins from the ER to the parasitophorous vacuole and given HSP101's specificity for the EXP2-PTEX150 subcomplex, this provides a mechanism for how exported proteins are specifically targeted to PTEX for translocation into the erythrocyte." @default.
- W4220958770 created "2022-04-03" @default.
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- W4220958770 date "2022-02-22" @default.
- W4220958770 modified "2023-10-02" @default.
- W4220958770 title "A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell" @default.
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- W4220958770 cites W1877187568 @default.
- W4220958770 cites W1913821607 @default.
- W4220958770 cites W1930569326 @default.
- W4220958770 cites W1967100058 @default.
- W4220958770 cites W1969503178 @default.
- W4220958770 cites W1973541716 @default.
- W4220958770 cites W1979637363 @default.
- W4220958770 cites W1983035189 @default.
- W4220958770 cites W1983588814 @default.
- W4220958770 cites W1984136987 @default.
- W4220958770 cites W1994599344 @default.
- W4220958770 cites W2001670495 @default.
- W4220958770 cites W2004803790 @default.
- W4220958770 cites W2015141554 @default.
- W4220958770 cites W2015578372 @default.
- W4220958770 cites W2023408468 @default.
- W4220958770 cites W2026186074 @default.
- W4220958770 cites W2028217000 @default.
- W4220958770 cites W2043873794 @default.
- W4220958770 cites W2046199004 @default.
- W4220958770 cites W2050196930 @default.
- W4220958770 cites W2051569783 @default.
- W4220958770 cites W2068928962 @default.
- W4220958770 cites W2070484082 @default.
- W4220958770 cites W2075117194 @default.
- W4220958770 cites W2077013312 @default.
- W4220958770 cites W2078432628 @default.
- W4220958770 cites W2079580216 @default.
- W4220958770 cites W2081640564 @default.
- W4220958770 cites W2086438483 @default.
- W4220958770 cites W2094739779 @default.
- W4220958770 cites W2096496718 @default.
- W4220958770 cites W2097432311 @default.
- W4220958770 cites W2101664493 @default.
- W4220958770 cites W2112047656 @default.
- W4220958770 cites W2112798524 @default.
- W4220958770 cites W2113498649 @default.
- W4220958770 cites W2117050306 @default.
- W4220958770 cites W2117312427 @default.
- W4220958770 cites W2120348909 @default.
- W4220958770 cites W2124765402 @default.
- W4220958770 cites W2131117238 @default.
- W4220958770 cites W2146620718 @default.
- W4220958770 cites W2151602360 @default.
- W4220958770 cites W2156160408 @default.
- W4220958770 cites W2158718118 @default.
- W4220958770 cites W2284697540 @default.
- W4220958770 cites W2322062798 @default.
- W4220958770 cites W2391733055 @default.
- W4220958770 cites W2538382920 @default.
- W4220958770 cites W2590802442 @default.
- W4220958770 cites W2592229839 @default.
- W4220958770 cites W2592912173 @default.
- W4220958770 cites W2726949767 @default.
- W4220958770 cites W2796820203 @default.
- W4220958770 cites W2801955897 @default.
- W4220958770 cites W2886540578 @default.
- W4220958770 cites W2887998543 @default.
- W4220958770 cites W2888208011 @default.
- W4220958770 cites W2901391548 @default.
- W4220958770 cites W2901891997 @default.
- W4220958770 cites W2910757401 @default.
- W4220958770 cites W2948752231 @default.
- W4220958770 cites W2955044791 @default.
- W4220958770 cites W3003736310 @default.
- W4220958770 cites W3016328580 @default.
- W4220958770 cites W3033485143 @default.
- W4220958770 cites W3140953426 @default.
- W4220958770 doi "https://doi.org/10.1371/journal.ppat.1009977" @default.
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- W4220958770 hasPublicationYear "2022" @default.
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