FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220996963> ?p ?o ?g. }

• W4220996963 abstract "Following their production in the testis, spermatozoa enter the epididymis where they gain their motility and fertilizing abilities. This post-testicular maturation coincides with sperm epigenetic profile changes that influence progeny outcome. While recent studies highlighted the dynamics of small non-coding RNAs in maturing spermatozoa, little is known regarding sperm methylation changes and their impact at the post-fertilization level. Fluorescence-activated cell sorting (FACS) was used to purify spermatozoa from the testis and different epididymal segments (i.e., caput, corpus and cauda) of CAG/su9-DsRed2; Acr3-EGFP transgenic mice in order to map out sperm methylome dynamics. Reduced representation bisulfite sequencing (RRBS-Seq) performed on DNA from these respective sperm populations indicated that high methylation changes were observed between spermatozoa from the caput vs. testis with 5,546 entries meeting our threshold values (q value <0.01, methylation difference above 25%). Most of these changes were transitory during epididymal sperm maturation according to the low number of entries identified between spermatozoa from cauda vs. testis. According to enzymatic and sperm/epididymal fluid co-incubation assays, (de)methylases were not found responsible for these sperm methylation changes. Instead, we identified that a subpopulation of caput spermatozoa displayed distinct methylation marks that were susceptible to sperm DNAse treatment and accounted for the DNA methylation profile changes observed in the proximal epididymis. Our results support the paradigm that a fraction of caput spermatozoa has a higher propensity to bind extracellular DNA, a phenomenon responsible for the sperm methylome variations observed at the post-testicular level. Further investigating the degree of conservation of this sperm heterogeneity in human will eventually provide new considerations regarding sperm selection procedures used in fertility clinics." @default.
• W4220996963 created "2022-04-03" @default.
• W4220996963 creator A5011459941 @default.
• W4220996963 creator A5037991705 @default.
• W4220996963 creator A5064530138 @default.
• W4220996963 creator A5064715796 @default.
• W4220996963 creator A5071487081 @default.
• W4220996963 date "2022-03-22" @default.
• W4220996963 modified "2023-10-16" @default.
• W4220996963 title "Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities" @default.
• W4220996963 cites W1979909569 @default.
• W4220996963 cites W1987557775 @default.
• W4220996963 cites W1991880159 @default.
• W4220996963 cites W2016819518 @default.
• W4220996963 cites W2017278367 @default.
• W4220996963 cites W2018304597 @default.
• W4220996963 cites W2022736447 @default.
• W4220996963 cites W2026343134 @default.
• W4220996963 cites W2049696888 @default.
• W4220996963 cites W2054191153 @default.
• W4220996963 cites W2063083693 @default.
• W4220996963 cites W2067633155 @default.
• W4220996963 cites W2084203180 @default.
• W4220996963 cites W2096391240 @default.
• W4220996963 cites W2101177916 @default.
• W4220996963 cites W2101228550 @default.
• W4220996963 cites W2119896245 @default.
• W4220996963 cites W2122032927 @default.
• W4220996963 cites W2124876474 @default.
• W4220996963 cites W2131271579 @default.
• W4220996963 cites W2131374955 @default.
• W4220996963 cites W2143511252 @default.
• W4220996963 cites W2150194502 @default.
• W4220996963 cites W2153523586 @default.
• W4220996963 cites W2168980816 @default.
• W4220996963 cites W2169474580 @default.
• W4220996963 cites W2170767694 @default.
• W4220996963 cites W2276004791 @default.
• W4220996963 cites W2325267338 @default.
• W4220996963 cites W2328076936 @default.
• W4220996963 cites W2331561267 @default.
• W4220996963 cites W2342433045 @default.
• W4220996963 cites W2346964985 @default.
• W4220996963 cites W2435911538 @default.
• W4220996963 cites W2554177165 @default.
• W4220996963 cites W2613822734 @default.
• W4220996963 cites W2767789945 @default.
• W4220996963 cites W2793133836 @default.
• W4220996963 cites W2800816467 @default.
• W4220996963 cites W2890158603 @default.
• W4220996963 cites W2902372981 @default.
• W4220996963 cites W2909484358 @default.
• W4220996963 cites W2952567878 @default.
• W4220996963 cites W2979933334 @default.
• W4220996963 cites W2989852253 @default.
• W4220996963 cites W2992389467 @default.
• W4220996963 cites W3046119185 @default.
• W4220996963 cites W3113496724 @default.
• W4220996963 cites W3133903516 @default.
• W4220996963 cites W3158038341 @default.
• W4220996963 cites W3199156700 @default.
• W4220996963 cites W3204678950 @default.
• W4220996963 doi "https://doi.org/10.3389/fcell.2022.834519" @default.
• W4220996963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35392175" @default.
• W4220996963 hasPublicationYear "2022" @default.
• W4220996963 type Work @default.
• W4220996963 citedByCount "4" @default.
• W4220996963 countsByYear W42209969632022 @default.
• W4220996963 countsByYear W42209969632023 @default.
• W4220996963 crossrefType "journal-article" @default.
• W4220996963 hasAuthorship W4220996963A5011459941 @default.
• W4220996963 hasAuthorship W4220996963A5037991705 @default.
• W4220996963 hasAuthorship W4220996963A5064530138 @default.
• W4220996963 hasAuthorship W4220996963A5064715796 @default.
• W4220996963 hasAuthorship W4220996963A5071487081 @default.
• W4220996963 hasBestOaLocation W42209969631 @default.
• W4220996963 hasConcept C104317684 @default.
• W4220996963 hasConcept C150194340 @default.
• W4220996963 hasConcept C16685009 @default.
• W4220996963 hasConcept C190727270 @default.
• W4220996963 hasConcept C2781087480 @default.
• W4220996963 hasConcept C2781254305 @default.
• W4220996963 hasConcept C33288867 @default.
• W4220996963 hasConcept C41091548 @default.
• W4220996963 hasConcept C54355233 @default.
• W4220996963 hasConcept C71924100 @default.
• W4220996963 hasConcept C86803240 @default.
• W4220996963 hasConceptScore W4220996963C104317684 @default.
• W4220996963 hasConceptScore W4220996963C150194340 @default.
• W4220996963 hasConceptScore W4220996963C16685009 @default.
• W4220996963 hasConceptScore W4220996963C190727270 @default.
• W4220996963 hasConceptScore W4220996963C2781087480 @default.
• W4220996963 hasConceptScore W4220996963C2781254305 @default.
• W4220996963 hasConceptScore W4220996963C33288867 @default.
• W4220996963 hasConceptScore W4220996963C41091548 @default.
• W4220996963 hasConceptScore W4220996963C54355233 @default.
• W4220996963 hasConceptScore W4220996963C71924100 @default.
• W4220996963 hasConceptScore W4220996963C86803240 @default.
• W4220996963 hasFunder F4320319939 @default.
• W4220996963 hasLocation W42209969631 @default.

• next