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• W4220997684 abstract "To understand general features in evolution of kinetochore organization, investigating a wide range of mitotic mechanisms in various non-model eukaryotes is necessary. A binucleate flagellate Giardia intestinalis is a representative of highly divergent eukaryotic lineage of Metamonads. FIB/SEM tomography was used to investigate ultrastructural details of its mitotic architecture, including kinetochores. Giardia undergoes semi-open mitosis, with the nuclear envelope remaining intact except for polar fenestrae, allowing microtubules to enter the nucleoplasm. At the onset of mitosis, the nuclear envelope bends inward, forming a concave depression at the spindle poles. Spindle microtubules emanate from a cytoplasmic fuzzy microtubule organizing center near the flagellar basal bodies. Kinetochoral microtubules enter the nucleoplasm and bind to kinetochores. A small bipartite kinetochore composed of a dense inner disk, approximately 46 nm in diameter, and a two-armed outer fork, is attached to just one microtubule. To our knowledge, this is the first in situ evidence of a one-microtubule attachment to a kinetochore, which could represent a basic eukaryotic situation." @default.
• W4220997684 created "2022-04-03" @default.
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• W4220997684 date "2022-04-01" @default.
• W4220997684 modified "2023-09-27" @default.
• W4220997684 title "In situ Visualization of a Simple Bipartite Kinetochore With A Single Microtubule Attachment in Giardia intestinalis (Metamonada)" @default.
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• W4220997684 doi "https://doi.org/10.1016/j.ejcb.2022.151217" @default.
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