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- W4221002967 abstract "Cryptocaryon irritans are the main pathogens of “white spot disease” on the body surface of marine teleosts, but the seawater environment affects pathogen development and virulence. This study investigated C. irritans tomont formation, cell division, hatching, and infectivity to Larimichthys crocea under eight salinity gradients (S 0 , S 5 , S 10 , S 20 , S 30 , S 40 , S 45 , and S 50 ) to understand how C. irritans grows, develops and harms host fish in a variable natural environment. The results showed that the protomont hatching rate in S 5 , S 10 , S 20 and S 30 significantly increased over time, but the highest and lowest values appeared in S 20 and S 30 , respectively; and the hatching rate in S 20 is significantly higher than S 30 .The rate of tomont formation increased over treatment time, and the highest and lowest rates were observed in S 20 and S 0 groups, respectively. Seawater at extreme salinity (S 0, S 40 , S 45 , and S 50 ) significantly reduced the encystment duration to <5 min. In addition, the tomont division rate at S 5 , S 10 , S 20 , S 30 , S 40 and S 45 significantly increased over time, and the highest and the lowest values were recorded in S 20 and S 45 , respectively; and the division rate at S 20 is significantly higher than S 45 . The hatching rate in S 5 , S 10 , S 20 , S 30 , and S 40 also significantly increased over time, but the highest and lowest values appeared in S 20 and S 5 , respectively; and the hatching rate in S 20 is significantly higher than S 5 . The tomonts treated with seawater of 5–45‰ salinity divided evidently, but most tomonts under 0 and 50‰ salinity dissolved. TEM observation showed the pellicular alveoli of tomonts in S 40 and S 45 were wider than S 20 , the chromatin was loose, and the cyst wall was separately compressed into thin-layered layers. Finally, the aspect ratios of the theronts hatched at S 5 , S 10 , S 20 , S 30 , and S 40 significantly increased than those at S 20 , and the theronts became long and thin. The infectivity of the theronts hatched from tomonts under S 5 and S 40 salinity treatments were significantly lower than the control (S 20 ) because of the changes of theront number and morphology. In conclusion, salinity changes regulate the formation and development of C. irritans tomonts. Low (S 5 ) or high (S 40 ) salinity inhibits the infectivity of incubated theronts. • The Cryptocaryon irritans protomonts rapidly encysted at high salinity. • Salinity changes resulted in a close arrangement of cyst wall layers, widening pellicular alveoli, and chromatin dispersions. • Salinity levels of 5and 40‰ significantly reduced the infectivity of the hatched theronts." @default.
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- W4221002967 date "2022-05-01" @default.
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- W4221002967 title "Salinity regulates the formation and hatching of Cryptocaryon irritans tomonts, affecting infectivity to Larimichthys crocea" @default.
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- W4221002967 doi "https://doi.org/10.1016/j.aquaculture.2022.738166" @default.
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