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- W4221012247 abstract "Transgenesis in model organisms is an essential tool for determining the function of protein-coding genes and non-coding regulatory regions. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extra-chromosomal arrays, but transgenes in arrays are frequently mosaic, over-expressed in some tissues, and silenced in the germline. Here, we describe methods to insert single-copy transgenes into specific genomic locations (MosSCI) or random locations (miniMos) using Mos1 transposons. Single-copy insertions allow expression at endogenous levels, expression in the germline, and identification of active and repressed regions of the genome." @default.
- W4221012247 created "2022-04-03" @default.
- W4221012247 creator A5010599646 @default.
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- W4221012247 date "2022-01-01" @default.
- W4221012247 modified "2023-09-24" @default.
- W4221012247 title "Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans" @default.
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- W4221012247 doi "https://doi.org/10.1007/978-1-0716-2181-3_12" @default.
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