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- W4221023941 abstract "The bacterium Staphylococcus saprophyticus (S. saprophyticus) is a common cause of urinary tract infections (UTI) in the community. To determine the effect of siRNA on the mRNA of the msrA gene in S. saprophyticus, 21-23 bp small interfering RNA (siRNA) duplexes were constructed against the mRNA of the msrA gene. The effect of siRNA on msrA mRNA expression was determined using reverse transcription PCR (RT-PCR). To assess changes in biofilm formation (BF) in response to siRNA activity, the usual tube technique was adopted. In vitro, msrA-siRNAs inhibited S. saprophyticus mRNA expression and activity. The efficacy of siRNA was determined by comparing the BF in S. saprophyticus before and after msrA-siRNA was introduced into the bacteria. In this investigation, two msrA-siRNA sequences, siRNA1 and siRNA2, were employed. qRT-PCR revealed that two msrA-siRNA sequences significantly suppressed the expression of msrA-mRNA, P = (0.010 and 0.002 ) respectively at (P< 0.05) comparison with control. Regarding the BF results after treatment with two siRNA sequences, they were 5/6 (83.5%) and 4/6 (67%) negative formation and 1/6 (16.5%) and 2/6 (33%) positive formation, respectively compared with control." @default.
- W4221023941 created "2022-04-03" @default.
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- W4221023941 date "2022-03-20" @default.
- W4221023941 modified "2023-09-26" @default.
- W4221023941 title "The role of siRNA in inhibition the biofilm formation as a first line of antibiotic resistance by regulation the MsrA drug efflux pump in Staphylococcus saprophyticus" @default.
- W4221023941 doi "https://doi.org/10.53730/ijhs.v6ns1.4904" @default.
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