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- W4221036664 abstract "Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verification to determine indels in one or both alleles by Sanger sequencing. This strategy is based on the efficiency of DNA editing, avoids antibiotic selection, and bypasses the need for cell sorting." @default.
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- W4221036664 date "2022-03-01" @default.
- W4221036664 modified "2023-10-14" @default.
- W4221036664 title "A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system" @default.
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- W4221036664 doi "https://doi.org/10.1016/j.xpro.2022.101216" @default.
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