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- W4221083513 abstract "Abstract Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers, and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms." @default.
- W4221083513 created "2022-04-03" @default.
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- W4221083513 date "2022-03-17" @default.
- W4221083513 modified "2023-10-10" @default.
- W4221083513 title "CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells" @default.
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- W4221083513 doi "https://doi.org/10.1186/s13059-022-02642-w" @default.
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