FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4221084896> ?p ?o ?g. }

• W4221084896 endingPage "S31" @default.
• W4221084896 startingPage "S30" @default.
• W4221084896 abstract "Abstract Introduction Burn shock is one of the most serious and complex complications suffered by patients following thermal injury. Endothelial dysfunction may play a role in the pathogenesis of burn shock. However, the mechanisms underlying the contribution to pathophysiology are still largely unknown. Previous studies have shown a connection between the rearrangement of cytoskeletal elements leading to increased vascular permeability. The aim of this study was to examine the differential expression of genes involved in cytoskeletal arrangement in endothelial cell monolayers treated with plasma from burn patients. Methods Human umbilical vein endothelial cells (HUVECs) were seeded into transwell plates to form confluent monolayers. Plasma was collected from burn patients 4 hours post-admission. HUVEC cells were exposed to 10% multi-donor pooled healthy human plasma (HHP) or burn patient plasma. Monolayers were subsequently incubated with FIT-C Dextran (40,000 kD) for 2 hours. Monolayer permeability was measured with indices calculated by normalizing values to blank wells (transwell inserts) and HHP-treated monolayer FIT-C diffusion. RNA was isolated from these same cells that had increased monolayer permeability and PCR analysis was carried out using an 84 gene array of human cytoskeletal regulators (Qiagen). A Ct value of 35 was used to indicate expression and a fold change of 1.5 to indicate differential expression in the control vs. injured groups. Results Four burn patient plasma samples were utilized to create injuries. Patients were mostly male (75%) with a mean age of 50±20 years and mean %TBSA burn of 37±34%. Differential gene expression in burn vs. HHP was compared. Ten genes showed significant upregulation (ARHGDIB, AURKA, AURKB, CCNB2, CIT, IQGAP2, VASP, SSH2, MYLK and DIAPH1). Four genes showed significant downregulation in (FSCN2, ARHGAP6, CYFIP2, CCNA1). Monolayer permeability indices showed statistically significant increases when compared to controls ranging from 3-13.33% (p &lt; .05). Conclusions The interplay of burn shock and endothelial dysfunction remains a complex process of which much is unknown. However, RNA analyses of burn patient plasma reveals involvement of multiple cytoskeletal regulators. Furthermore, all these samples show a concurrent increase in permeability indices when compared to controls, further strengthening the association between cytoskeletal rearrangement and endotheliopathy. Future research to better understand the specifics of these pathways could help aid in the development of more targeted treatments of endotheliopathy and burn shock. Cytoskeletal rearrangement may be an interesting target for future work to understand this mechanistic interplay." @default.
• W4221084896 created "2022-04-03" @default.
• W4221084896 creator A5030596322 @default.
• W4221084896 creator A5041572522 @default.
• W4221084896 creator A5063369009 @default.
• W4221084896 creator A5071958879 @default.
• W4221084896 creator A5073719913 @default.
• W4221084896 date "2022-03-23" @default.
• W4221084896 modified "2023-10-16" @default.
• W4221084896 title "44 Endothelial Monolayers Treated with Burn Patient Plasma Exhibit Differential Gene Expression Linked to Cytoskeletal Rearrangement" @default.
• W4221084896 doi "https://doi.org/10.1093/jbcr/irac012.047" @default.
• W4221084896 hasPublicationYear "2022" @default.
• W4221084896 type Work @default.
• W4221084896 citedByCount "0" @default.
• W4221084896 crossrefType "journal-article" @default.
• W4221084896 hasAuthorship W4221084896A5030596322 @default.
• W4221084896 hasAuthorship W4221084896A5041572522 @default.
• W4221084896 hasAuthorship W4221084896A5063369009 @default.
• W4221084896 hasAuthorship W4221084896A5071958879 @default.
• W4221084896 hasAuthorship W4221084896A5073719913 @default.
• W4221084896 hasBestOaLocation W42210848961 @default.
• W4221084896 hasConcept C104317684 @default.
• W4221084896 hasConcept C113045295 @default.
• W4221084896 hasConcept C123012128 @default.
• W4221084896 hasConcept C142669718 @default.
• W4221084896 hasConcept C142724271 @default.
• W4221084896 hasConcept C1491633281 @default.
• W4221084896 hasConcept C150194340 @default.
• W4221084896 hasConcept C16685009 @default.
• W4221084896 hasConcept C202751555 @default.
• W4221084896 hasConcept C203014093 @default.
• W4221084896 hasConcept C2776657240 @default.
• W4221084896 hasConcept C2777411675 @default.
• W4221084896 hasConcept C2777834122 @default.
• W4221084896 hasConcept C2780942790 @default.
• W4221084896 hasConcept C55493867 @default.
• W4221084896 hasConcept C7070889 @default.
• W4221084896 hasConcept C71924100 @default.
• W4221084896 hasConcept C86803240 @default.
• W4221084896 hasConcept C95444343 @default.
• W4221084896 hasConceptScore W4221084896C104317684 @default.
• W4221084896 hasConceptScore W4221084896C113045295 @default.
• W4221084896 hasConceptScore W4221084896C123012128 @default.
• W4221084896 hasConceptScore W4221084896C142669718 @default.
• W4221084896 hasConceptScore W4221084896C142724271 @default.
• W4221084896 hasConceptScore W4221084896C1491633281 @default.
• W4221084896 hasConceptScore W4221084896C150194340 @default.
• W4221084896 hasConceptScore W4221084896C16685009 @default.
• W4221084896 hasConceptScore W4221084896C202751555 @default.
• W4221084896 hasConceptScore W4221084896C203014093 @default.
• W4221084896 hasConceptScore W4221084896C2776657240 @default.
• W4221084896 hasConceptScore W4221084896C2777411675 @default.
• W4221084896 hasConceptScore W4221084896C2777834122 @default.
• W4221084896 hasConceptScore W4221084896C2780942790 @default.
• W4221084896 hasConceptScore W4221084896C55493867 @default.
• W4221084896 hasConceptScore W4221084896C7070889 @default.
• W4221084896 hasConceptScore W4221084896C71924100 @default.
• W4221084896 hasConceptScore W4221084896C86803240 @default.
• W4221084896 hasConceptScore W4221084896C95444343 @default.
• W4221084896 hasIssue "Supplement_1" @default.
• W4221084896 hasLocation W42210848961 @default.
• W4221084896 hasLocation W42210848962 @default.
• W4221084896 hasOpenAccess W4221084896 @default.
• W4221084896 hasPrimaryLocation W42210848961 @default.
• W4221084896 hasRelatedWork W2019446856 @default.
• W4221084896 hasRelatedWork W2057602456 @default.
• W4221084896 hasRelatedWork W2087300502 @default.
• W4221084896 hasRelatedWork W2110392942 @default.
• W4221084896 hasRelatedWork W2171826138 @default.
• W4221084896 hasRelatedWork W2365831865 @default.
• W4221084896 hasRelatedWork W2379306340 @default.
• W4221084896 hasRelatedWork W2595053779 @default.
• W4221084896 hasRelatedWork W2921402103 @default.
• W4221084896 hasRelatedWork W2953911840 @default.
• W4221084896 hasVolume "43" @default.
• W4221084896 isParatext "false" @default.
• W4221084896 isRetracted "false" @default.
• W4221084896 workType "article" @default.