Matches in SemOpenAlex for { <https://semopenalex.org/work/W4221096170> ?p ?o ?g. }
- W4221096170 endingPage "389" @default.
- W4221096170 startingPage "381" @default.
- W4221096170 abstract "Type I restriction-modification enzymes are oligomeric proteins composed of methylation (M), DNA sequence-recognition (S), and restriction (R) subunits. The different bipartite DNA sequences of 2-4 consecutive bases are recognized by two discerned target recognition domains (TRDs) located at the two-helix bundle of the two conserved regions (CRs). Two M-subunits and a single S-subunit form an oligomeric protein that functions as a methyltransferase (M2S1 MTase). Here, we present the crystal structure of the intact MTase from Vibrio vulnificus YJ016 in complex with the DNA-mimicking Ocr protein and the S-adenosyl-L-homocysteine (SAH). This MTase includes the M-domain with a helix tail (M-tail helix) and the S1/2-domain of a TRD and a CR α-helix. The Ocr binds to the cleft of the TRD surface and SAH is located in the pocket within the M-domain. The solution- and negative-staining electron microscopy-based reconstructed (M1S1/2)2 structure reveals a symmetric (S1/2)2 assembly using two CR-helices and two M-tail helices as a pivot, which is plausible for recognizing two DNA regions of same sequence. The conformational flexibility of the minimal M1S1/2 MTase dimer indicates a particular state resembling the structure of M2S1 MTases." @default.
- W4221096170 created "2022-04-03" @default.
- W4221096170 creator A5022813627 @default.
- W4221096170 creator A5023244709 @default.
- W4221096170 creator A5025007734 @default.
- W4221096170 creator A5050561032 @default.
- W4221096170 creator A5050806700 @default.
- W4221096170 creator A5051301807 @default.
- W4221096170 creator A5054300719 @default.
- W4221096170 creator A5058555804 @default.
- W4221096170 creator A5074578302 @default.
- W4221096170 creator A5078479213 @default.
- W4221096170 creator A5079004842 @default.
- W4221096170 creator A5081997233 @default.
- W4221096170 creator A5089770011 @default.
- W4221096170 creator A5091554389 @default.
- W4221096170 date "2022-05-01" @default.
- W4221096170 modified "2023-10-18" @default.
- W4221096170 title "Structural features of a minimal intact methyltransferase of a type I restriction-modification system" @default.
- W4221096170 cites W1524357749 @default.
- W4221096170 cites W1539796472 @default.
- W4221096170 cites W1542411948 @default.
- W4221096170 cites W1738997773 @default.
- W4221096170 cites W1741082581 @default.
- W4221096170 cites W1763400538 @default.
- W4221096170 cites W1867958153 @default.
- W4221096170 cites W1964482368 @default.
- W4221096170 cites W1969192031 @default.
- W4221096170 cites W1974277594 @default.
- W4221096170 cites W1992191979 @default.
- W4221096170 cites W1996466878 @default.
- W4221096170 cites W2014070665 @default.
- W4221096170 cites W2016208316 @default.
- W4221096170 cites W2017763991 @default.
- W4221096170 cites W2022749781 @default.
- W4221096170 cites W2027170515 @default.
- W4221096170 cites W2041625042 @default.
- W4221096170 cites W2044145557 @default.
- W4221096170 cites W2044370121 @default.
- W4221096170 cites W2060807817 @default.
- W4221096170 cites W2096040465 @default.
- W4221096170 cites W2098571197 @default.
- W4221096170 cites W2114725566 @default.
- W4221096170 cites W2117191068 @default.
- W4221096170 cites W2119661887 @default.
- W4221096170 cites W2124983865 @default.
- W4221096170 cites W2138181691 @default.
- W4221096170 cites W2144081223 @default.
- W4221096170 cites W2144377439 @default.
- W4221096170 cites W2149885731 @default.
- W4221096170 cites W2161861736 @default.
- W4221096170 cites W2171434257 @default.
- W4221096170 cites W2172075081 @default.
- W4221096170 cites W2180229411 @default.
- W4221096170 cites W225079696 @default.
- W4221096170 cites W2339273139 @default.
- W4221096170 cites W2568179435 @default.
- W4221096170 cites W2587625522 @default.
- W4221096170 cites W2762409145 @default.
- W4221096170 cites W2884421468 @default.
- W4221096170 cites W3030046774 @default.
- W4221096170 cites W3103292928 @default.
- W4221096170 cites W620516222 @default.
- W4221096170 doi "https://doi.org/10.1016/j.ijbiomac.2022.03.115" @default.
- W4221096170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35337914" @default.
- W4221096170 hasPublicationYear "2022" @default.
- W4221096170 type Work @default.
- W4221096170 citedByCount "0" @default.
- W4221096170 crossrefType "journal-article" @default.
- W4221096170 hasAuthorship W4221096170A5022813627 @default.
- W4221096170 hasAuthorship W4221096170A5023244709 @default.
- W4221096170 hasAuthorship W4221096170A5025007734 @default.
- W4221096170 hasAuthorship W4221096170A5050561032 @default.
- W4221096170 hasAuthorship W4221096170A5050806700 @default.
- W4221096170 hasAuthorship W4221096170A5051301807 @default.
- W4221096170 hasAuthorship W4221096170A5054300719 @default.
- W4221096170 hasAuthorship W4221096170A5058555804 @default.
- W4221096170 hasAuthorship W4221096170A5074578302 @default.
- W4221096170 hasAuthorship W4221096170A5078479213 @default.
- W4221096170 hasAuthorship W4221096170A5079004842 @default.
- W4221096170 hasAuthorship W4221096170A5081997233 @default.
- W4221096170 hasAuthorship W4221096170A5089770011 @default.
- W4221096170 hasAuthorship W4221096170A5091554389 @default.
- W4221096170 hasConcept C100594029 @default.
- W4221096170 hasConcept C104292427 @default.
- W4221096170 hasConcept C104317684 @default.
- W4221096170 hasConcept C12554922 @default.
- W4221096170 hasConcept C128319531 @default.
- W4221096170 hasConcept C153911025 @default.
- W4221096170 hasConcept C178790620 @default.
- W4221096170 hasConcept C185592680 @default.
- W4221096170 hasConcept C18903297 @default.
- W4221096170 hasConcept C2778530040 @default.
- W4221096170 hasConcept C2779546866 @default.
- W4221096170 hasConcept C2779965526 @default.
- W4221096170 hasConcept C2779979206 @default.
- W4221096170 hasConcept C33288867 @default.
- W4221096170 hasConcept C552990157 @default.