Matches in SemOpenAlex for { <https://semopenalex.org/work/W4221104037> ?p ?o ?g. }
- W4221104037 endingPage "1355" @default.
- W4221104037 startingPage "1338" @default.
- W4221104037 abstract "RNA interference (RNAi) is a powerful tool capable of targeting virtually any protein without time-consuming and expensive drug development studies. However, due to obstacles facing efficient and safe delivery, RNAi-based therapeutic approach remains a challenge. Herein, we have designed and synthesized a number of disulfide-constraining cyclic and hybrid peptides using tryptophan and arginine residues. Our hypothesis was that peptide structures would undergo reduction by intracellular glutathione (more abundant in cancer cells) and unpack the small interfering RNA (siRNA) from the peptide/siRNA complexes. A subset of newly developed peptides (specifically, C4 and H4) exhibited effective cellular internalization of siRNA (∼70% of the cell population; monitored by flow cytometry and confocal microscopy), the capability of protecting siRNA against early degradation by nucleases (monitored by gel electrophoresis), minimal cytotoxicity in selected cell lines (studied by cell viability and LC50 calculations), and efficient protein silencing by 70-75% reduction in the expression of targeting signal transducer and activator of transcription 3 (STAT3) in human triple-negative breast cancer (TNBC) MDA-MB-231 cells, analyzed using the Western blot technique. Our results indicate the birth of a promising new family of siRNA delivery systems that are capable of safe and efficient delivery, even in the presence of nucleases." @default.
- W4221104037 created "2022-04-03" @default.
- W4221104037 creator A5011814485 @default.
- W4221104037 creator A5031842435 @default.
- W4221104037 creator A5032667090 @default.
- W4221104037 creator A5033339559 @default.
- W4221104037 creator A5044903753 @default.
- W4221104037 creator A5059669763 @default.
- W4221104037 creator A5060177451 @default.
- W4221104037 creator A5089612182 @default.
- W4221104037 date "2022-03-29" @default.
- W4221104037 modified "2023-09-28" @default.
- W4221104037 title "Redox-Responsive Disulfide Cyclic Peptides: A New Strategy for siRNA Delivery" @default.
- W4221104037 cites W1548640328 @default.
- W4221104037 cites W1647075334 @default.
- W4221104037 cites W1974933012 @default.
- W4221104037 cites W1982195500 @default.
- W4221104037 cites W2000729091 @default.
- W4221104037 cites W2011617167 @default.
- W4221104037 cites W2013947977 @default.
- W4221104037 cites W2020763460 @default.
- W4221104037 cites W2047036303 @default.
- W4221104037 cites W2052312685 @default.
- W4221104037 cites W2060797161 @default.
- W4221104037 cites W2067845253 @default.
- W4221104037 cites W2072715976 @default.
- W4221104037 cites W2075567991 @default.
- W4221104037 cites W2076324415 @default.
- W4221104037 cites W2083577929 @default.
- W4221104037 cites W2101135540 @default.
- W4221104037 cites W2104050632 @default.
- W4221104037 cites W2108614912 @default.
- W4221104037 cites W2111193378 @default.
- W4221104037 cites W2157377945 @default.
- W4221104037 cites W2159166506 @default.
- W4221104037 cites W2159955926 @default.
- W4221104037 cites W2166618356 @default.
- W4221104037 cites W2173073542 @default.
- W4221104037 cites W2326627549 @default.
- W4221104037 cites W2327335574 @default.
- W4221104037 cites W2562506588 @default.
- W4221104037 cites W2563642244 @default.
- W4221104037 cites W2620755219 @default.
- W4221104037 cites W2624979306 @default.
- W4221104037 cites W2725117793 @default.
- W4221104037 cites W2766133412 @default.
- W4221104037 cites W2783713318 @default.
- W4221104037 cites W2883151927 @default.
- W4221104037 cites W2897366926 @default.
- W4221104037 cites W2906007725 @default.
- W4221104037 cites W2938255464 @default.
- W4221104037 cites W2985133717 @default.
- W4221104037 cites W2992823069 @default.
- W4221104037 cites W3030502181 @default.
- W4221104037 cites W3033107534 @default.
- W4221104037 cites W3035589805 @default.
- W4221104037 cites W3112558789 @default.
- W4221104037 cites W3122288075 @default.
- W4221104037 cites W3128794291 @default.
- W4221104037 cites W3137683229 @default.
- W4221104037 cites W3200517152 @default.
- W4221104037 cites W3207886513 @default.
- W4221104037 cites W3215094233 @default.
- W4221104037 cites W4205142613 @default.
- W4221104037 cites W4211039187 @default.
- W4221104037 doi "https://doi.org/10.1021/acs.molpharmaceut.1c00879" @default.
- W4221104037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35347995" @default.
- W4221104037 hasPublicationYear "2022" @default.
- W4221104037 type Work @default.
- W4221104037 citedByCount "4" @default.
- W4221104037 countsByYear W42211040372022 @default.
- W4221104037 countsByYear W42211040372023 @default.
- W4221104037 crossrefType "journal-article" @default.
- W4221104037 hasAuthorship W4221104037A5011814485 @default.
- W4221104037 hasAuthorship W4221104037A5031842435 @default.
- W4221104037 hasAuthorship W4221104037A5032667090 @default.
- W4221104037 hasAuthorship W4221104037A5033339559 @default.
- W4221104037 hasAuthorship W4221104037A5044903753 @default.
- W4221104037 hasAuthorship W4221104037A5059669763 @default.
- W4221104037 hasAuthorship W4221104037A5060177451 @default.
- W4221104037 hasAuthorship W4221104037A5089612182 @default.
- W4221104037 hasConcept C104317684 @default.
- W4221104037 hasConcept C109316439 @default.
- W4221104037 hasConcept C119056186 @default.
- W4221104037 hasConcept C139770010 @default.
- W4221104037 hasConcept C144024400 @default.
- W4221104037 hasConcept C1491633281 @default.
- W4221104037 hasConcept C149923435 @default.
- W4221104037 hasConcept C153911025 @default.
- W4221104037 hasConcept C166703698 @default.
- W4221104037 hasConcept C185592680 @default.
- W4221104037 hasConcept C202751555 @default.
- W4221104037 hasConcept C22615655 @default.
- W4221104037 hasConcept C2908647359 @default.
- W4221104037 hasConcept C553184892 @default.
- W4221104037 hasConcept C55493867 @default.
- W4221104037 hasConcept C67705224 @default.
- W4221104037 hasConcept C79879829 @default.