Matches in SemOpenAlex for { <https://semopenalex.org/work/W4221133234> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4221133234 abstract "Metastatic tumor cell invasion into interstitial tissue is a mechanochemical process that responds to tissue cues and further involves proteolytic remodeling of the tumor stroma. How matrix density, tissue guidance and the ability of proteolytic tissue remodeling cooperate and determine decision-making of invading tumor cells in complex-structured three-dimensional (3D) tissue remains unclear. We here developed a collagen-based invasion assay containing a guiding interface of low collagen density adjacent to randomly organized 3D fibrillar lattice and examined the invasion of melanoma cells from multicellular spheroids in response to matrix density, guidance cues and collagenolysis. After 48 hours of culture, two invasion niches developed, (i) sheet-like collective migration along the interface and (ii) single cell- and strand-like invasion into randomly organized 3D matrix. High collagen density impeded migration into the random matrix, whereas migration along a high-density collagen interface was increased compared to the low-density matrix assay. In silico analysis predicted that facilitated interface migration in high-density matrix depended on physical guidance without collagen degradation, whereas migration into randomly organized matrix was strongly dependent on collagenolysis. When tested in 3D culture, inhibition of matrix metalloprotease (MMP)-mediated collagen degradation compromised migration into random matrix in dependence of density, whereas interface-guided migration remained effective. In conclusion, with increasing tissue density, matrix cues bordered by dense matrix, but not randomly organized matrix, support effective MMP-independent migration. This identifies the topology of interstitial tissue a primary determinant of switch behaviors between MMP-dependent and MMP-independent cancer cell invasion." @default.
- W4221133234 created "2022-04-03" @default.
- W4221133234 creator A5002126634 @default.
- W4221133234 creator A5006268986 @default.
- W4221133234 creator A5019911064 @default.
- W4221133234 creator A5026527299 @default.
- W4221133234 creator A5034059805 @default.
- W4221133234 creator A5037998198 @default.
- W4221133234 creator A5049658141 @default.
- W4221133234 creator A5081906909 @default.
- W4221133234 creator A5085068731 @default.
- W4221133234 creator A5087177103 @default.
- W4221133234 date "2022-03-18" @default.
- W4221133234 modified "2023-09-26" @default.
- W4221133234 title "Extracellular matrix guidance determines proteolytic and non-proteolytic cancer cell patterning" @default.
- W4221133234 doi "https://doi.org/10.1101/2022.03.16.484647" @default.
- W4221133234 hasPublicationYear "2022" @default.
- W4221133234 type Work @default.
- W4221133234 citedByCount "0" @default.
- W4221133234 crossrefType "posted-content" @default.
- W4221133234 hasAuthorship W4221133234A5002126634 @default.
- W4221133234 hasAuthorship W4221133234A5006268986 @default.
- W4221133234 hasAuthorship W4221133234A5019911064 @default.
- W4221133234 hasAuthorship W4221133234A5026527299 @default.
- W4221133234 hasAuthorship W4221133234A5034059805 @default.
- W4221133234 hasAuthorship W4221133234A5037998198 @default.
- W4221133234 hasAuthorship W4221133234A5049658141 @default.
- W4221133234 hasAuthorship W4221133234A5081906909 @default.
- W4221133234 hasAuthorship W4221133234A5085068731 @default.
- W4221133234 hasAuthorship W4221133234A5087177103 @default.
- W4221133234 hasBestOaLocation W42211332341 @default.
- W4221133234 hasConcept C106487976 @default.
- W4221133234 hasConcept C109523444 @default.
- W4221133234 hasConcept C12554922 @default.
- W4221133234 hasConcept C137738243 @default.
- W4221133234 hasConcept C1491633281 @default.
- W4221133234 hasConcept C181199279 @default.
- W4221133234 hasConcept C185592680 @default.
- W4221133234 hasConcept C189165786 @default.
- W4221133234 hasConcept C3019223276 @default.
- W4221133234 hasConcept C43617362 @default.
- W4221133234 hasConcept C55493867 @default.
- W4221133234 hasConcept C86803240 @default.
- W4221133234 hasConcept C95444343 @default.
- W4221133234 hasConceptScore W4221133234C106487976 @default.
- W4221133234 hasConceptScore W4221133234C109523444 @default.
- W4221133234 hasConceptScore W4221133234C12554922 @default.
- W4221133234 hasConceptScore W4221133234C137738243 @default.
- W4221133234 hasConceptScore W4221133234C1491633281 @default.
- W4221133234 hasConceptScore W4221133234C181199279 @default.
- W4221133234 hasConceptScore W4221133234C185592680 @default.
- W4221133234 hasConceptScore W4221133234C189165786 @default.
- W4221133234 hasConceptScore W4221133234C3019223276 @default.
- W4221133234 hasConceptScore W4221133234C43617362 @default.
- W4221133234 hasConceptScore W4221133234C55493867 @default.
- W4221133234 hasConceptScore W4221133234C86803240 @default.
- W4221133234 hasConceptScore W4221133234C95444343 @default.
- W4221133234 hasLocation W42211332341 @default.
- W4221133234 hasOpenAccess W4221133234 @default.
- W4221133234 hasPrimaryLocation W42211332341 @default.
- W4221133234 hasRelatedWork W1437588494 @default.
- W4221133234 hasRelatedWork W1754220768 @default.
- W4221133234 hasRelatedWork W1977020874 @default.
- W4221133234 hasRelatedWork W1983658560 @default.
- W4221133234 hasRelatedWork W1992755940 @default.
- W4221133234 hasRelatedWork W2075729495 @default.
- W4221133234 hasRelatedWork W2084295528 @default.
- W4221133234 hasRelatedWork W2156234950 @default.
- W4221133234 hasRelatedWork W2416128704 @default.
- W4221133234 hasRelatedWork W2944434395 @default.
- W4221133234 isParatext "false" @default.
- W4221133234 isRetracted "false" @default.
- W4221133234 workType "article" @default.