Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223419422> ?p ?o ?g. }
- W4223419422 endingPage "679" @default.
- W4223419422 startingPage "668" @default.
- W4223419422 abstract "Cell cultured meat (CCM) production is an innovative technology that does not depend on livestock farming practices to produce meat. The construction of structured CCM requires a three-dimensional (3D) scaffold to mimic the extracellular matrix to provide mechanical support for the cells. Furthermore, the 3D scaffolds should be edible and have good biocompatibility and tissue-like texture. Here, we demonstrated a 3D edible chitosan‑sodium alginate-collagen/gelatin (CS-SA-Col/Gel) scaffold that can support the adhesion and proliferation of porcine skeletal muscle satellite cells, culminating in the construction of a structured CCM model. The 3D edible scaffolds were prepared by freeze-drying using electrostatic interactions between chitosan and sodium alginate. Initially, the physicochemical properties and structural characteristics of different scaffolds were explored, and the biocompatibility of the scaffolds was evaluated using the C2C12 cell model. The results showed that the 2-CS-SA-Col1-Gel scaffold provided stable mechanical support and abundant adhesion sites for the cells. Subsequently, we inoculated porcine skeletal muscle satellite cells on the 2-CS-SA-Col1-Gel scaffold and induced differentiation for a total of 14 days. Immunofluorescence staining results showed cytoskeleton formation, and Western blotting (WB) and qPCR results showed upregulation of skeletal proteins and myogenic genes. Ultimately, the structured CCM model has similar textural properties (chewiness, springiness and resilience) and appearance to those of fresh pork. In conclusion, the method of constructing 3D edible scaffolds to prepare structured CCM models exhibits the potential to produce cell cultured meat." @default.
- W4223419422 created "2022-04-14" @default.
- W4223419422 creator A5012713786 @default.
- W4223419422 creator A5022308338 @default.
- W4223419422 creator A5037458498 @default.
- W4223419422 creator A5045692426 @default.
- W4223419422 creator A5050574516 @default.
- W4223419422 creator A5057560415 @default.
- W4223419422 creator A5065220600 @default.
- W4223419422 creator A5067689026 @default.
- W4223419422 creator A5078143614 @default.
- W4223419422 creator A5079674922 @default.
- W4223419422 creator A5089614699 @default.
- W4223419422 date "2022-06-01" @default.
- W4223419422 modified "2023-10-17" @default.
- W4223419422 title "Chitosan‑sodium alginate-collagen/gelatin three-dimensional edible scaffolds for building a structured model for cell cultured meat" @default.
- W4223419422 cites W1974543599 @default.
- W4223419422 cites W1979126565 @default.
- W4223419422 cites W1984685797 @default.
- W4223419422 cites W1985575261 @default.
- W4223419422 cites W1986697405 @default.
- W4223419422 cites W1987190449 @default.
- W4223419422 cites W1988432563 @default.
- W4223419422 cites W1998566162 @default.
- W4223419422 cites W2010481935 @default.
- W4223419422 cites W2013084580 @default.
- W4223419422 cites W2019415517 @default.
- W4223419422 cites W2045295282 @default.
- W4223419422 cites W2078718449 @default.
- W4223419422 cites W2079252549 @default.
- W4223419422 cites W2082084058 @default.
- W4223419422 cites W2095112270 @default.
- W4223419422 cites W2113042424 @default.
- W4223419422 cites W2126048177 @default.
- W4223419422 cites W2157059052 @default.
- W4223419422 cites W2204329593 @default.
- W4223419422 cites W2337981703 @default.
- W4223419422 cites W2395046586 @default.
- W4223419422 cites W2586443454 @default.
- W4223419422 cites W2594152260 @default.
- W4223419422 cites W2608297406 @default.
- W4223419422 cites W2610933022 @default.
- W4223419422 cites W2772579246 @default.
- W4223419422 cites W2789257332 @default.
- W4223419422 cites W2790681883 @default.
- W4223419422 cites W2794528450 @default.
- W4223419422 cites W2800381029 @default.
- W4223419422 cites W2809323699 @default.
- W4223419422 cites W2884775052 @default.
- W4223419422 cites W2904514829 @default.
- W4223419422 cites W2907974660 @default.
- W4223419422 cites W2955385594 @default.
- W4223419422 cites W2961946656 @default.
- W4223419422 cites W2965537493 @default.
- W4223419422 cites W2981015863 @default.
- W4223419422 cites W3007526073 @default.
- W4223419422 cites W3008038668 @default.
- W4223419422 cites W3013866919 @default.
- W4223419422 cites W3020913786 @default.
- W4223419422 cites W3134551257 @default.
- W4223419422 cites W3165760367 @default.
- W4223419422 cites W3177009627 @default.
- W4223419422 cites W4252179483 @default.
- W4223419422 doi "https://doi.org/10.1016/j.ijbiomac.2022.04.052" @default.
- W4223419422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35413327" @default.
- W4223419422 hasPublicationYear "2022" @default.
- W4223419422 type Work @default.
- W4223419422 citedByCount "19" @default.
- W4223419422 countsByYear W42234194222022 @default.
- W4223419422 countsByYear W42234194222023 @default.
- W4223419422 crossrefType "journal-article" @default.
- W4223419422 hasAuthorship W4223419422A5012713786 @default.
- W4223419422 hasAuthorship W4223419422A5022308338 @default.
- W4223419422 hasAuthorship W4223419422A5037458498 @default.
- W4223419422 hasAuthorship W4223419422A5045692426 @default.
- W4223419422 hasAuthorship W4223419422A5050574516 @default.
- W4223419422 hasAuthorship W4223419422A5057560415 @default.
- W4223419422 hasAuthorship W4223419422A5065220600 @default.
- W4223419422 hasAuthorship W4223419422A5067689026 @default.
- W4223419422 hasAuthorship W4223419422A5078143614 @default.
- W4223419422 hasAuthorship W4223419422A5079674922 @default.
- W4223419422 hasAuthorship W4223419422A5089614699 @default.
- W4223419422 hasConcept C12554922 @default.
- W4223419422 hasConcept C136229726 @default.
- W4223419422 hasConcept C142724271 @default.
- W4223419422 hasConcept C1491633281 @default.
- W4223419422 hasConcept C178790620 @default.
- W4223419422 hasConcept C185592680 @default.
- W4223419422 hasConcept C189165786 @default.
- W4223419422 hasConcept C202751555 @default.
- W4223419422 hasConcept C2777230088 @default.
- W4223419422 hasConcept C2778823387 @default.
- W4223419422 hasConcept C2779732960 @default.
- W4223419422 hasConcept C3763915 @default.
- W4223419422 hasConcept C49892992 @default.
- W4223419422 hasConcept C55493867 @default.
- W4223419422 hasConcept C60278653 @default.
- W4223419422 hasConcept C71924100 @default.