Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223438217> ?p ?o ?g. }
- W4223438217 endingPage "123479" @default.
- W4223438217 startingPage "123479" @default.
- W4223438217 abstract "Electrochemical polymerase chain reaction (PCR) represents a valid alternative to the optical-based PCR due to reduced costs of signaling labels, use of simpler instrumentation, and possibility of miniaturization and portability of the systems, which can facilitate decentralized detection. The high intrinsic electroactivity and strong linear relationship between the material concentration and its redox signal suggest a possible use of oxidized nanocarbon materials as electroactive tags for PCR. Herein, we compared three different nanographene oxide materials namely nGO-1, nGO-2 and nGO-3 as signaling tags for the detection of genetically modified organisms (GMO) by electrochemical PCR. The three materials differ in size, chemical composition as well as type and amount of oxygen functionalities verified by extensive characterization with X-ray photoelectron spectroscopy (XPS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), transmission electron microscopy (TEM) and electrochemical methods. A sense primer sequence belonging to the Cauliflower Mosaic Virus 35S promoter (a common genetic marker for GMO screening) was used to conjugate to the nanocarbon materials by carbodiimide chemistry before PCR amplification with a biotinylated antisense strand. Finally, the amplified electroactive PCR product was detected, where the reduction signal derived from the electrochemically reducible oxygenated functionalities on the nanocarbon material surface was directly correlated to the presence of GMO. Overall, we were able to correlate the different material characteristics with their performance as electroactive labels and identify the nanocarbon material that exhibits the highest potential to be used as innovative electroactive label for PCR in the amplification and detection of the selected target sequence." @default.
- W4223438217 created "2022-04-14" @default.
- W4223438217 creator A5023462468 @default.
- W4223438217 creator A5044448228 @default.
- W4223438217 creator A5047602859 @default.
- W4223438217 creator A5061372715 @default.
- W4223438217 creator A5077357570 @default.
- W4223438217 date "2022-08-01" @default.
- W4223438217 modified "2023-10-17" @default.
- W4223438217 title "Electroactive nanocarbon materials as signaling tags for electrochemical PCR" @default.
- W4223438217 cites W1094558270 @default.
- W4223438217 cites W1971476731 @default.
- W4223438217 cites W1971715932 @default.
- W4223438217 cites W1975750858 @default.
- W4223438217 cites W1998201662 @default.
- W4223438217 cites W2002808856 @default.
- W4223438217 cites W2016831113 @default.
- W4223438217 cites W2023643635 @default.
- W4223438217 cites W2040550439 @default.
- W4223438217 cites W2042578974 @default.
- W4223438217 cites W2044172403 @default.
- W4223438217 cites W2052412786 @default.
- W4223438217 cites W2055681620 @default.
- W4223438217 cites W2063215525 @default.
- W4223438217 cites W2064464358 @default.
- W4223438217 cites W2076664050 @default.
- W4223438217 cites W2084350317 @default.
- W4223438217 cites W2085477476 @default.
- W4223438217 cites W2089817402 @default.
- W4223438217 cites W2098059495 @default.
- W4223438217 cites W2103761174 @default.
- W4223438217 cites W2109357726 @default.
- W4223438217 cites W2123056314 @default.
- W4223438217 cites W2124824337 @default.
- W4223438217 cites W2128612221 @default.
- W4223438217 cites W2135479172 @default.
- W4223438217 cites W2139036614 @default.
- W4223438217 cites W2148033822 @default.
- W4223438217 cites W2149193628 @default.
- W4223438217 cites W2151311304 @default.
- W4223438217 cites W2151341480 @default.
- W4223438217 cites W2156012944 @default.
- W4223438217 cites W2159493817 @default.
- W4223438217 cites W2165370588 @default.
- W4223438217 cites W2166243022 @default.
- W4223438217 cites W2167640692 @default.
- W4223438217 cites W2168483951 @default.
- W4223438217 cites W2168648389 @default.
- W4223438217 cites W2298323016 @default.
- W4223438217 cites W2325582004 @default.
- W4223438217 cites W2330029947 @default.
- W4223438217 cites W2330515363 @default.
- W4223438217 cites W2331539008 @default.
- W4223438217 cites W2333023959 @default.
- W4223438217 cites W2481179302 @default.
- W4223438217 cites W2555408999 @default.
- W4223438217 cites W2555519714 @default.
- W4223438217 cites W2564875409 @default.
- W4223438217 cites W2741120152 @default.
- W4223438217 cites W2752907281 @default.
- W4223438217 cites W2766085136 @default.
- W4223438217 cites W2766719126 @default.
- W4223438217 cites W2767471979 @default.
- W4223438217 cites W2770780495 @default.
- W4223438217 cites W2770816340 @default.
- W4223438217 cites W2782917857 @default.
- W4223438217 cites W2793922288 @default.
- W4223438217 cites W2798003276 @default.
- W4223438217 cites W2889855672 @default.
- W4223438217 cites W2891429033 @default.
- W4223438217 cites W2937876125 @default.
- W4223438217 cites W2944928533 @default.
- W4223438217 cites W2988373537 @default.
- W4223438217 cites W3003264330 @default.
- W4223438217 cites W3028938709 @default.
- W4223438217 cites W3044899395 @default.
- W4223438217 cites W3048459635 @default.
- W4223438217 doi "https://doi.org/10.1016/j.talanta.2022.123479" @default.
- W4223438217 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35436731" @default.
- W4223438217 hasPublicationYear "2022" @default.
- W4223438217 type Work @default.
- W4223438217 citedByCount "1" @default.
- W4223438217 countsByYear W42234382172023 @default.
- W4223438217 crossrefType "journal-article" @default.
- W4223438217 hasAuthorship W4223438217A5023462468 @default.
- W4223438217 hasAuthorship W4223438217A5044448228 @default.
- W4223438217 hasAuthorship W4223438217A5047602859 @default.
- W4223438217 hasAuthorship W4223438217A5061372715 @default.
- W4223438217 hasAuthorship W4223438217A5077357570 @default.
- W4223438217 hasConcept C127413603 @default.
- W4223438217 hasConcept C147789679 @default.
- W4223438217 hasConcept C160892712 @default.
- W4223438217 hasConcept C171250308 @default.
- W4223438217 hasConcept C17525397 @default.
- W4223438217 hasConcept C175708663 @default.
- W4223438217 hasConcept C178790620 @default.
- W4223438217 hasConcept C185592680 @default.
- W4223438217 hasConcept C192562407 @default.