FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223442948> ?p ?o ?g. }

• W4223442948 abstract "Vaccinia virus (VV), the prototypic poxvirus, encodes a repertoire of proteins responsible for the metabolism of its large dsDNA genome. Previous work has furthered our understanding of how poxviruses replicate and recombine their genomes, but little is known about whether the poxvirus genome undergoes DNA repair. Our studies here are aimed at understanding how VV responds to exogenous DNA damage introduced by UV irradiation. Irradiation of cells prior to infection decreased protein synthesis and led to an ∼12-fold reduction in viral yield. On top of these cell-specific insults, irradiation of VV infections at 4 h postinfection (hpi) introduced both cyclobutene pyrimidine dimer (CPD) and 6,4-photoproduct (6,4-PP) lesions into the viral genome led to a nearly complete halt to further DNA synthesis and to a further reduction in viral yield (∼35-fold). DNA lesions persisted throughout infection and were indeed present in the genomes encapsidated into nascent virions. Depletion of several cellular proteins that mediate nucleotide excision repair (XP-A, -F, and -G) did not render viral infections hypersensitive to UV. We next investigated whether viral proteins were involved in combatting DNA damage. Infections performed with a virus lacking the A50 DNA ligase were moderately hypersensitive to UV irradiation (∼3-fold). More strikingly, when the DNA polymerase inhibitor cytosine arabinoside (araC) was added to wild-type infections at the time of UV irradiation (4 hpi), an even greater hypersensitivity to UV irradiation was seen (∼11-fold). Virions produced under the latter condition contained elevated levels of CPD adducts, strongly suggesting that the viral polymerase contributes to the repair of UV lesions introduced into the viral genome. IMPORTANCE Poxviruses remain of significant interest because of their continuing clinical relevance, their utility for the development of vaccines and oncolytic therapies, and their illustration of fundamental principles of viral replication and virus/cell interactions. These viruses are unique in that they replicate exclusively in the cytoplasm of infected mammalian cells, providing novel challenges for DNA viruses. How poxviruses replicate, recombine, and possibly repair their genomes is still only partially understood. Using UV irradiation as a form of exogenous DNA damage, we have examined how vaccinia virus metabolizes its genome following insult. We show that even UV irradiation of cells prior to infection diminishes viral yield, while UV irradiation during infection damages the genome, causes a halt in DNA accumulation, and reduces the viral yield more severely. Furthermore, we show that viral proteins, but not the cellular machinery, contribute to a partial repair of the viral genome following UV irradiation." @default.
• W4223442948 created "2022-04-14" @default.
• W4223442948 creator A5001731234 @default.
• W4223442948 creator A5028086587 @default.
• W4223442948 date "2022-05-11" @default.
• W4223442948 modified "2023-10-10" @default.
• W4223442948 title "UV Irradiation of Vaccinia Virus-Infected Cells Impairs Cellular Functions, Introduces Lesions into the Viral Genome, and Uncovers Repair Capabilities for the Viral Replication Machinery" @default.
• W4223442948 cites W1484966744 @default.
• W4223442948 cites W1486346025 @default.
• W4223442948 cites W1497540493 @default.
• W4223442948 cites W1546888157 @default.
• W4223442948 cites W1625224420 @default.
• W4223442948 cites W1670233224 @default.
• W4223442948 cites W1759069886 @default.
• W4223442948 cites W1832749657 @default.
• W4223442948 cites W1833536387 @default.
• W4223442948 cites W1886853229 @default.
• W4223442948 cites W1887119421 @default.
• W4223442948 cites W1892311206 @default.
• W4223442948 cites W1957143406 @default.
• W4223442948 cites W1971425868 @default.
• W4223442948 cites W1973807140 @default.
• W4223442948 cites W1977848671 @default.
• W4223442948 cites W1978674906 @default.
• W4223442948 cites W1979490109 @default.
• W4223442948 cites W1982251983 @default.
• W4223442948 cites W1983515856 @default.
• W4223442948 cites W1988582914 @default.
• W4223442948 cites W1989718073 @default.
• W4223442948 cites W1993956311 @default.
• W4223442948 cites W1998032842 @default.
• W4223442948 cites W1998696660 @default.
• W4223442948 cites W2003875234 @default.
• W4223442948 cites W2026743476 @default.
• W4223442948 cites W2036371955 @default.
• W4223442948 cites W2045563677 @default.
• W4223442948 cites W2051559242 @default.
• W4223442948 cites W2063623284 @default.
• W4223442948 cites W2072000449 @default.
• W4223442948 cites W2074632322 @default.
• W4223442948 cites W2085936072 @default.
• W4223442948 cites W2087090258 @default.
• W4223442948 cites W2087413282 @default.
• W4223442948 cites W2091230055 @default.
• W4223442948 cites W2092333256 @default.
• W4223442948 cites W2092351149 @default.
• W4223442948 cites W2097124161 @default.
• W4223442948 cites W2097826722 @default.
• W4223442948 cites W2099579351 @default.
• W4223442948 cites W2100193190 @default.
• W4223442948 cites W2100856120 @default.
• W4223442948 cites W2101224698 @default.
• W4223442948 cites W2108163253 @default.
• W4223442948 cites W2113384721 @default.
• W4223442948 cites W2116524791 @default.
• W4223442948 cites W2130027092 @default.
• W4223442948 cites W2143308044 @default.
• W4223442948 cites W2148492018 @default.
• W4223442948 cites W2160028232 @default.
• W4223442948 cites W2161616221 @default.
• W4223442948 cites W2167317609 @default.
• W4223442948 cites W2170942873 @default.
• W4223442948 cites W2327611812 @default.
• W4223442948 cites W2765947814 @default.
• W4223442948 cites W2967431278 @default.
• W4223442948 cites W89499173 @default.
• W4223442948 doi "https://doi.org/10.1128/jvi.02137-21" @default.
• W4223442948 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35404095" @default.
• W4223442948 hasPublicationYear "2022" @default.
• W4223442948 type Work @default.
• W4223442948 citedByCount "2" @default.
• W4223442948 countsByYear W42234429482023 @default.
• W4223442948 crossrefType "journal-article" @default.
• W4223442948 hasAuthorship W4223442948A5001731234 @default.
• W4223442948 hasAuthorship W4223442948A5028086587 @default.
• W4223442948 hasBestOaLocation W42234429482 @default.
• W4223442948 hasConcept C104317684 @default.
• W4223442948 hasConcept C134935766 @default.
• W4223442948 hasConcept C140704245 @default.
• W4223442948 hasConcept C141231307 @default.
• W4223442948 hasConcept C143425029 @default.
• W4223442948 hasConcept C153911025 @default.
• W4223442948 hasConcept C159047783 @default.
• W4223442948 hasConcept C167975912 @default.
• W4223442948 hasConcept C182615771 @default.
• W4223442948 hasConcept C2522874641 @default.
• W4223442948 hasConcept C2781356689 @default.
• W4223442948 hasConcept C40767141 @default.
• W4223442948 hasConcept C54355233 @default.
• W4223442948 hasConcept C552990157 @default.
• W4223442948 hasConcept C73573662 @default.
• W4223442948 hasConcept C86803240 @default.
• W4223442948 hasConceptScore W4223442948C104317684 @default.
• W4223442948 hasConceptScore W4223442948C134935766 @default.
• W4223442948 hasConceptScore W4223442948C140704245 @default.
• W4223442948 hasConceptScore W4223442948C141231307 @default.
• W4223442948 hasConceptScore W4223442948C143425029 @default.
• W4223442948 hasConceptScore W4223442948C153911025 @default.
• W4223442948 hasConceptScore W4223442948C159047783 @default.
• W4223442948 hasConceptScore W4223442948C167975912 @default.

• next