FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223449840> ?p ?o ?g. }

• W4223449840 abstract "Cryptococcus neoformans is an environmental yeast and an opportunistic human pathogen. The ability to cause disease depends on the ability to adapt to the human host. Previous studies implicated infectivity-related kinase 3 (IRK3, CNAG_03048) as required for establishing an infection. We genetically and biochemically characterized IRK3 as a gluconate kinase and propose the name GNK1. This metabolic enzyme utilizes gluconate to produce 6-phosphogluconate as part of the alternative oxidative phase of the pentose phosphate pathway (AOXPPP). The presence of GNK1 confirms that the AOXPPP is present and able to compensate for loss of the traditional OXPPP, providing an explanation for its nonessentiality. C. neoformans can utilize gluconate as an alternative carbon source in a GNK1-dependent manner. In our efforts to understand the role of GNK1 in host adaptation and virulence, we found that GNK1-deficient mutants have variable virulence and carbon dioxide tolerance across multiple strains, suggesting that second site mutations frequently interact with GNK1 deletion mutations. In our effort to isolate these genetic loci by backcrossing experiments, we discovered that GNK1-deficient strains are unable to sporulate. These data suggest that gluconate metabolism is critical for sporulation of C. neoformans. IMPORTANCE Cryptococcus neoformans is a fungal pathogen that contributes to nearly 180,000 deaths annually. We characterized a gene named GNK1 that appears to interact with other genetic loci involved with the ability of C. neoformans to act as a pathogen. While these interacting genetic loci remain elusive, we discovered that GNK1 plays roles in both metabolism and mating/sporulation. Further interrogation of the mechanistic role for GNK1 in sexual reproduction may uncover a larger network of genes that are important for host adaptation and virulence." @default.
• W4223449840 created "2022-04-14" @default.
• W4223449840 creator A5034492000 @default.
• W4223449840 creator A5045253526 @default.
• W4223449840 creator A5062972515 @default.
• W4223449840 creator A5076058258 @default.
• W4223449840 date "2022-04-27" @default.
• W4223449840 modified "2023-10-16" @default.
• W4223449840 title "Gluconate Kinase Is Required for Gluconate Assimilation and Sporulation in Cryptococcus neoformans" @default.
• W4223449840 cites W1673751863 @default.
• W4223449840 cites W1968110247 @default.
• W4223449840 cites W1985214490 @default.
• W4223449840 cites W2002155770 @default.
• W4223449840 cites W2003079967 @default.
• W4223449840 cites W2006386982 @default.
• W4223449840 cites W2035612098 @default.
• W4223449840 cites W2094041961 @default.
• W4223449840 cites W2109118083 @default.
• W4223449840 cites W2114445049 @default.
• W4223449840 cites W2120926466 @default.
• W4223449840 cites W2141967665 @default.
• W4223449840 cites W2149216252 @default.
• W4223449840 cites W2163333404 @default.
• W4223449840 cites W2523840231 @default.
• W4223449840 cites W2724527698 @default.
• W4223449840 cites W2785844090 @default.
• W4223449840 cites W2895515366 @default.
• W4223449840 cites W2910430054 @default.
• W4223449840 cites W2954171301 @default.
• W4223449840 cites W3012883959 @default.
• W4223449840 cites W3137405931 @default.
• W4223449840 doi "https://doi.org/10.1128/spectrum.00301-22" @default.
• W4223449840 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35412378" @default.
• W4223449840 hasPublicationYear "2022" @default.
• W4223449840 type Work @default.
• W4223449840 citedByCount "3" @default.
• W4223449840 countsByYear W42234498402023 @default.
• W4223449840 crossrefType "journal-article" @default.
• W4223449840 hasAuthorship W4223449840A5034492000 @default.
• W4223449840 hasAuthorship W4223449840A5045253526 @default.
• W4223449840 hasAuthorship W4223449840A5062972515 @default.
• W4223449840 hasAuthorship W4223449840A5076058258 @default.
• W4223449840 hasBestOaLocation W42234498403 @default.
• W4223449840 hasConcept C104317684 @default.
• W4223449840 hasConcept C181199279 @default.
• W4223449840 hasConcept C20251656 @default.
• W4223449840 hasConcept C24527192 @default.
• W4223449840 hasConcept C2777893358 @default.
• W4223449840 hasConcept C2779413141 @default.
• W4223449840 hasConcept C3409486 @default.
• W4223449840 hasConcept C54355233 @default.
• W4223449840 hasConcept C55493867 @default.
• W4223449840 hasConcept C60987743 @default.
• W4223449840 hasConcept C86803240 @default.
• W4223449840 hasConcept C89423630 @default.
• W4223449840 hasConceptScore W4223449840C104317684 @default.
• W4223449840 hasConceptScore W4223449840C181199279 @default.
• W4223449840 hasConceptScore W4223449840C20251656 @default.
• W4223449840 hasConceptScore W4223449840C24527192 @default.
• W4223449840 hasConceptScore W4223449840C2777893358 @default.
• W4223449840 hasConceptScore W4223449840C2779413141 @default.
• W4223449840 hasConceptScore W4223449840C3409486 @default.
• W4223449840 hasConceptScore W4223449840C54355233 @default.
• W4223449840 hasConceptScore W4223449840C55493867 @default.
• W4223449840 hasConceptScore W4223449840C60987743 @default.
• W4223449840 hasConceptScore W4223449840C86803240 @default.
• W4223449840 hasConceptScore W4223449840C89423630 @default.
• W4223449840 hasFunder F4320337355 @default.
• W4223449840 hasIssue "2" @default.
• W4223449840 hasLocation W42234498401 @default.
• W4223449840 hasLocation W42234498402 @default.
• W4223449840 hasLocation W42234498403 @default.
• W4223449840 hasLocation W42234498404 @default.
• W4223449840 hasOpenAccess W4223449840 @default.
• W4223449840 hasPrimaryLocation W42234498401 @default.
• W4223449840 hasRelatedWork W1963570707 @default.
• W4223449840 hasRelatedWork W2005800004 @default.
• W4223449840 hasRelatedWork W2012907196 @default.
• W4223449840 hasRelatedWork W2036920350 @default.
• W4223449840 hasRelatedWork W2095908222 @default.
• W4223449840 hasRelatedWork W2168471667 @default.
• W4223449840 hasRelatedWork W2313503697 @default.
• W4223449840 hasRelatedWork W2944870338 @default.
• W4223449840 hasRelatedWork W4214826497 @default.
• W4223449840 hasRelatedWork W4226117608 @default.
• W4223449840 hasVolume "10" @default.
• W4223449840 isParatext "false" @default.
• W4223449840 isRetracted "false" @default.
• W4223449840 workType "article" @default.