Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223475910> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4223475910 endingPage "106" @default.
- W4223475910 startingPage "102" @default.
- W4223475910 abstract "Objective: To explore the development of a CAR-T cells targeting CLL-1 and verify its function. Methods: The expression levels of CLL-1 targets in cell lines and primary cells were detected by flow cytometry. A CLL-1 CAR vector was constructed, and the corresponding lentivirus was prepared. After infection and activation of T cells, CAR-T cells targeting CLL-1 were produced and their function was verified in vitro and in vivo. Results: CLL-1 was expressed in acute myeloid leukemia (AML) cell lines and primary AML cells. The transduction rate of the prepared CAR T cells was 77.82%. In AML cell lines and AML primary cells, CLL-1-targeting CAR-T cells significantly and specifically killed CLL-1-expressing cells. Compared to untransduced T cells, CAR-T cells killed target cells and secreted inflammatory cytokines, such as interleukin-6 and interferon-γ, at significantly higher levels (P<0.001) . In an in vivo human xenograft mouse model of AML, CLL-1 CAR-T cells also exhibited potent antileukemic activity and induced prolonged mouse survival compared with untransduced T cells [not reached vs 22 days (95%CI 19-24 days) , P=0.002]. Conclusion: CAR-T cells targeting CLL-1 have been successfully produced and have excellent functions.目的： 探索开发一种靶向CLL-1的嵌合抗原受体T细胞（CAR-T细胞）并验证其功能。 方法： 通过流式细胞术检测急性髓系白血病（AML）细胞系和AML原代细胞CLL-1靶点的表达水平。构建CLL-1 CAR载体并制备出相应慢病毒，感染激活后T细胞生产出CAR-T细胞，并通过体外和体内实验验证CLL-1 CAR-T细胞的功能。 结果： AML细胞系和原代AML细胞中均表达CLL-1。制备的CLL-1 CAR-T细胞转导率为77.82%，在AML细胞系以及AML原代细胞中，CLL-1 CAR-T细胞能明显特异性杀伤表达CLL-1的靶细胞系和原代肿瘤细胞。相对于未转导的T细胞，CLL-1 CAR-T细胞在杀伤靶细胞和原代肿瘤细胞时分泌IL-6、IFN-γ等细胞因子水平更高（P值均<0.001）。在AML人源性异种移植小鼠模型中，相对于未转导的T细胞，CLL-1 CAR-T细胞表现出有效的抗白血病活性并延长小鼠存活时间[未达到对22（95%CI 19~24）d，P=0.002]。 结论： 靶向CLL-1的CAR-T细胞成功开发并具有较好的肿瘤杀伤作用。." @default.
- W4223475910 created "2022-04-15" @default.
- W4223475910 creator A5026513884 @default.
- W4223475910 creator A5034649465 @default.
- W4223475910 creator A5080338000 @default.
- W4223475910 date "2022-02-14" @default.
- W4223475910 modified "2023-10-17" @default.
- W4223475910 title "[Development and functional verification of CAR-T cells targeting CLL-1]." @default.
- W4223475910 cites W1976187277 @default.
- W4223475910 cites W1994448489 @default.
- W4223475910 cites W2022881751 @default.
- W4223475910 cites W2026252733 @default.
- W4223475910 cites W2047205338 @default.
- W4223475910 cites W2565897110 @default.
- W4223475910 cites W2613026858 @default.
- W4223475910 cites W2621998980 @default.
- W4223475910 cites W2765972717 @default.
- W4223475910 cites W2783779434 @default.
- W4223475910 cites W2802646364 @default.
- W4223475910 cites W2895050534 @default.
- W4223475910 cites W2898794856 @default.
- W4223475910 cites W2913127457 @default.
- W4223475910 cites W2947566808 @default.
- W4223475910 cites W2995900562 @default.
- W4223475910 cites W3087497958 @default.
- W4223475910 cites W3092240902 @default.
- W4223475910 cites W3111839605 @default.
- W4223475910 cites W3128868608 @default.
- W4223475910 cites W3128956257 @default.
- W4223475910 cites W3130903278 @default.
- W4223475910 cites W3131786547 @default.
- W4223475910 cites W3132274017 @default.
- W4223475910 doi "https://doi.org/10.3760/cma.j.issn.0253-2727.2022.02.003" @default.
- W4223475910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35381669" @default.
- W4223475910 hasPublicationYear "2022" @default.
- W4223475910 type Work @default.
- W4223475910 citedByCount "0" @default.
- W4223475910 crossrefType "journal-article" @default.
- W4223475910 hasAuthorship W4223475910A5026513884 @default.
- W4223475910 hasAuthorship W4223475910A5034649465 @default.
- W4223475910 hasAuthorship W4223475910A5080338000 @default.
- W4223475910 hasConcept C129374314 @default.
- W4223475910 hasConcept C131665280 @default.
- W4223475910 hasConcept C185592680 @default.
- W4223475910 hasConcept C203014093 @default.
- W4223475910 hasConcept C2776090121 @default.
- W4223475910 hasConcept C2777938653 @default.
- W4223475910 hasConcept C2778461978 @default.
- W4223475910 hasConcept C2778729363 @default.
- W4223475910 hasConcept C502942594 @default.
- W4223475910 hasConcept C54355233 @default.
- W4223475910 hasConcept C553184892 @default.
- W4223475910 hasConcept C81885089 @default.
- W4223475910 hasConcept C86803240 @default.
- W4223475910 hasConcept C8891405 @default.
- W4223475910 hasConceptScore W4223475910C129374314 @default.
- W4223475910 hasConceptScore W4223475910C131665280 @default.
- W4223475910 hasConceptScore W4223475910C185592680 @default.
- W4223475910 hasConceptScore W4223475910C203014093 @default.
- W4223475910 hasConceptScore W4223475910C2776090121 @default.
- W4223475910 hasConceptScore W4223475910C2777938653 @default.
- W4223475910 hasConceptScore W4223475910C2778461978 @default.
- W4223475910 hasConceptScore W4223475910C2778729363 @default.
- W4223475910 hasConceptScore W4223475910C502942594 @default.
- W4223475910 hasConceptScore W4223475910C54355233 @default.
- W4223475910 hasConceptScore W4223475910C553184892 @default.
- W4223475910 hasConceptScore W4223475910C81885089 @default.
- W4223475910 hasConceptScore W4223475910C86803240 @default.
- W4223475910 hasConceptScore W4223475910C8891405 @default.
- W4223475910 hasIssue "2" @default.
- W4223475910 hasLocation W42234759101 @default.
- W4223475910 hasOpenAccess W4223475910 @default.
- W4223475910 hasPrimaryLocation W42234759101 @default.
- W4223475910 hasRelatedWork W1283782 @default.
- W4223475910 hasRelatedWork W19037421 @default.
- W4223475910 hasRelatedWork W1937448 @default.
- W4223475910 hasRelatedWork W3602028 @default.
- W4223475910 hasRelatedWork W40408739 @default.
- W4223475910 hasRelatedWork W44237016 @default.
- W4223475910 hasRelatedWork W6426471 @default.
- W4223475910 hasRelatedWork W814015 @default.
- W4223475910 hasRelatedWork W15988229 @default.
- W4223475910 hasRelatedWork W17880734 @default.
- W4223475910 hasVolume "43" @default.
- W4223475910 isParatext "false" @default.
- W4223475910 isRetracted "false" @default.
- W4223475910 workType "article" @default.