Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223476114> ?p ?o ?g. }
- W4223476114 endingPage "4321" @default.
- W4223476114 startingPage "4321" @default.
- W4223476114 abstract "Although the cat flea, Ctenocephalides felis, has been identified as the primary vector of Rickettsia felis, additional flea, tick, mite, and louse species have also been associated with this bacterium by molecular means; however, the role of these arthropods in the transmission of R. felis has not been clarified. Here, we succeeded in culture isolation of R. felis from a host-seeking castor bean tick, Ixodes ricinus, the most common tick in Slovakia. The bacterial isolation was performed on XTC-2 cells at 28 °C using the shell-vial technique. An evaluation of the growth properties was performed for both the XTC-2 and Vero cell lines. We observed R. felis in the infected host cells microscopically by Gimenez staining and immunofluorescence assay. The R. felis isolate was purified by gradient ultracentrifugation and visualized by electron microscopy. Fragments of the genes gltA, ompA, ompB, htrA, rpoB, sca4, rffE, and rrs were amplified and compared with the corresponding sequences of the type strain URRWXCal2 and other R. felis culture -isolated strains. We did not detect any nucleotide polymorphisms; however, plasmid pRFδ, characteristic of the standard strain, was absent in our isolate. Herein, we describe the first successful isolation and characterization of a tick-derived R. felis strain Danube, obtained from an I. ricinus nymph." @default.
- W4223476114 created "2022-04-15" @default.
- W4223476114 creator A5031684968 @default.
- W4223476114 creator A5062194803 @default.
- W4223476114 creator A5085236410 @default.
- W4223476114 creator A5086353622 @default.
- W4223476114 date "2022-04-04" @default.
- W4223476114 modified "2023-10-17" @default.
- W4223476114 title "Culture Isolate of Rickettsia felis from a Tick" @default.
- W4223476114 cites W1548849672 @default.
- W4223476114 cites W1559079979 @default.
- W4223476114 cites W1585980236 @default.
- W4223476114 cites W1625981166 @default.
- W4223476114 cites W1742606097 @default.
- W4223476114 cites W1758410558 @default.
- W4223476114 cites W1831195965 @default.
- W4223476114 cites W1894200179 @default.
- W4223476114 cites W1896994006 @default.
- W4223476114 cites W1943047008 @default.
- W4223476114 cites W1967643528 @default.
- W4223476114 cites W1968609971 @default.
- W4223476114 cites W1971761299 @default.
- W4223476114 cites W1973915560 @default.
- W4223476114 cites W1975023526 @default.
- W4223476114 cites W1980159166 @default.
- W4223476114 cites W1981290252 @default.
- W4223476114 cites W1984934742 @default.
- W4223476114 cites W1987140122 @default.
- W4223476114 cites W1988152273 @default.
- W4223476114 cites W1991073118 @default.
- W4223476114 cites W2000796069 @default.
- W4223476114 cites W2004873125 @default.
- W4223476114 cites W2009636263 @default.
- W4223476114 cites W2019621675 @default.
- W4223476114 cites W2025049939 @default.
- W4223476114 cites W2030136297 @default.
- W4223476114 cites W2031550213 @default.
- W4223476114 cites W2033834342 @default.
- W4223476114 cites W2036372901 @default.
- W4223476114 cites W2043189306 @default.
- W4223476114 cites W2044779859 @default.
- W4223476114 cites W2047516823 @default.
- W4223476114 cites W2053731760 @default.
- W4223476114 cites W2064189776 @default.
- W4223476114 cites W2067858365 @default.
- W4223476114 cites W2071631259 @default.
- W4223476114 cites W2084117379 @default.
- W4223476114 cites W2089112000 @default.
- W4223476114 cites W2098861019 @default.
- W4223476114 cites W2100952044 @default.
- W4223476114 cites W2103824582 @default.
- W4223476114 cites W2105280436 @default.
- W4223476114 cites W2110356129 @default.
- W4223476114 cites W2114247580 @default.
- W4223476114 cites W2117304322 @default.
- W4223476114 cites W2119798746 @default.
- W4223476114 cites W2121100483 @default.
- W4223476114 cites W2121794620 @default.
- W4223476114 cites W2122469844 @default.
- W4223476114 cites W2126017813 @default.
- W4223476114 cites W2133867125 @default.
- W4223476114 cites W2134496395 @default.
- W4223476114 cites W2134903315 @default.
- W4223476114 cites W2136765986 @default.
- W4223476114 cites W2138251583 @default.
- W4223476114 cites W2143825190 @default.
- W4223476114 cites W2144072424 @default.
- W4223476114 cites W2145015532 @default.
- W4223476114 cites W2145019681 @default.
- W4223476114 cites W2145025086 @default.
- W4223476114 cites W2152068449 @default.
- W4223476114 cites W2152459664 @default.
- W4223476114 cites W2153224807 @default.
- W4223476114 cites W2161880806 @default.
- W4223476114 cites W2164403670 @default.
- W4223476114 cites W2167506573 @default.
- W4223476114 cites W2167747529 @default.
- W4223476114 cites W2167922226 @default.
- W4223476114 cites W2192536738 @default.
- W4223476114 cites W2238079233 @default.
- W4223476114 cites W2341065086 @default.
- W4223476114 cites W2344406969 @default.
- W4223476114 cites W2345444988 @default.
- W4223476114 cites W2408146622 @default.
- W4223476114 cites W2409089547 @default.
- W4223476114 cites W2411989189 @default.
- W4223476114 cites W2413164758 @default.
- W4223476114 cites W2540836091 @default.
- W4223476114 cites W2565777672 @default.
- W4223476114 cites W2594725122 @default.
- W4223476114 cites W2604827439 @default.
- W4223476114 cites W2739789165 @default.
- W4223476114 cites W2765189162 @default.
- W4223476114 cites W2787887943 @default.
- W4223476114 cites W2904012303 @default.
- W4223476114 cites W2911351477 @default.
- W4223476114 cites W2980711919 @default.
- W4223476114 cites W2981851855 @default.