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- W4223526970 abstract "All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium." @default.
- W4223526970 created "2022-04-15" @default.
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- W4223526970 date "2022-05-01" @default.
- W4223526970 modified "2023-10-16" @default.
- W4223526970 title "Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology" @default.
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- W4223526970 doi "https://doi.org/10.1002/jbio.202100352" @default.
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