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- W4223534638 abstract "We describe an electrochemical strategy to transduce allosteric transcription factor (aTF) binding affinity to sense steroid hormones. Our approach utilizes square wave voltammetry to monitor changes in current output as a progesterone (PRG)-specific aTF (SRTF1) unbinds from the cognate DNA sequence in the presence of PRG. The sensor detects PRG in artificial urine samples with sufficient sensitivity suitable for clinical applications. Our results highlight the capability of using aTFs as the biorecognition elements to develop electrochemical point-of-care biosensors for the detection of small-molecule biomarkers and analytes." @default.
- W4223534638 created "2022-04-15" @default.
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- W4223534638 date "2022-04-12" @default.
- W4223534638 modified "2023-09-27" @default.
- W4223534638 title "An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone" @default.
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- W4223534638 doi "https://doi.org/10.1021/acssensors.2c00133" @default.
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