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• W4223603727 abstract "Membrane proteins (MPs) are essential in many cellular functions. To maintain proteostasis, MPs are downregulated via ubiquitination and degradation. Here, we describe an optimized protocol to analyze MP degradation using quantitative western blot and flow cytometry-based approaches. We use the degradation of Ypq1, a vacuole membrane lysine transporter, to demonstrate the protocol, which can be adapted for other organelle MPs and thus provide useful tools to study MP regulation in yeast and other model organisms. For complete details on the use and execution of this protocol, please refer to Arines et al. (2021) and Yang et al. (2020)." @default.
• W4223603727 created "2022-04-15" @default.
• W4223603727 creator A5003811156 @default.
• W4223603727 creator A5021384155 @default.
• W4223603727 date "2022-06-01" @default.
• W4223603727 modified "2023-10-04" @default.
• W4223603727 title "An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry" @default.
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• W4223603727 doi "https://doi.org/10.1016/j.xpro.2022.101274" @default.
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