Matches in SemOpenAlex for { <https://semopenalex.org/work/W4223971878> ?p ?o ?g. }
- W4223971878 endingPage "132919" @default.
- W4223971878 startingPage "132919" @default.
- W4223971878 abstract "Herein, we propose a sensitive fluorescent assay for organophosphorus pesticides (OPs) detection based on a novel strategy of activating the CRISPR-Cas12a system. Specifically, acetylcholinesterase (AChE) hydrolyzes acetylthiocholine into thiocholine (TCh). Subsequently, TCh induces the degradation of MnO2 nanosheets and generates sufficient Mn2+ ions to activate the Mn2+-dependent DNAzyme. Then, as the catalytic product of activated DNAzyme, the short DNA strand activates the CRISPR-Cas12a system to cleave the fluorophore-quencher-labeled DNA reporter (FQ) probe effectively; thus, increasing the fluorescence intensity (FI) in the solution. However, in the presence of OPs, the activity of AChE is suppressed, resulting in a decrease in FI. Under optimized conditions, the limits of detection for paraoxon, dichlorvos, and demeton were 270, 406, and 218 pg/mL, respectively. Benefiting from the outstanding MnO2 nanosheets properties and three rounds of enzymatic signal amplification, the proposed fluorescence assay holds great potential for the detection of OPs in agricultural products." @default.
- W4223971878 created "2022-04-19" @default.
- W4223971878 creator A5027950443 @default.
- W4223971878 creator A5031837561 @default.
- W4223971878 creator A5035754451 @default.
- W4223971878 creator A5040827219 @default.
- W4223971878 creator A5045297186 @default.
- W4223971878 creator A5050018342 @default.
- W4223971878 creator A5053771367 @default.
- W4223971878 creator A5059281655 @default.
- W4223971878 creator A5082086115 @default.
- W4223971878 creator A5082346338 @default.
- W4223971878 date "2022-09-01" @default.
- W4223971878 modified "2023-10-13" @default.
- W4223971878 title "CRISPR-Cas12a based fluorescence assay for organophosphorus pesticides in agricultural products" @default.
- W4223971878 cites W1998985533 @default.
- W4223971878 cites W2019994265 @default.
- W4223971878 cites W2077716320 @default.
- W4223971878 cites W2095525285 @default.
- W4223971878 cites W2101749257 @default.
- W4223971878 cites W2104527187 @default.
- W4223971878 cites W2113385123 @default.
- W4223971878 cites W2166139447 @default.
- W4223971878 cites W2475271036 @default.
- W4223971878 cites W2553371281 @default.
- W4223971878 cites W2570697704 @default.
- W4223971878 cites W2580171608 @default.
- W4223971878 cites W2765690574 @default.
- W4223971878 cites W2787024379 @default.
- W4223971878 cites W2921061236 @default.
- W4223971878 cites W2973049331 @default.
- W4223971878 cites W2982040007 @default.
- W4223971878 cites W2989918917 @default.
- W4223971878 cites W2992478910 @default.
- W4223971878 cites W3006453090 @default.
- W4223971878 cites W3007434817 @default.
- W4223971878 cites W3011255507 @default.
- W4223971878 cites W3016515039 @default.
- W4223971878 cites W3022068053 @default.
- W4223971878 cites W3023010695 @default.
- W4223971878 cites W3026380879 @default.
- W4223971878 cites W3037980422 @default.
- W4223971878 cites W3042243693 @default.
- W4223971878 cites W3042381012 @default.
- W4223971878 cites W3045975352 @default.
- W4223971878 cites W3082205315 @default.
- W4223971878 cites W3087905712 @default.
- W4223971878 cites W3088392422 @default.
- W4223971878 cites W3088783902 @default.
- W4223971878 cites W3093322893 @default.
- W4223971878 cites W3135999387 @default.
- W4223971878 cites W3184960742 @default.
- W4223971878 doi "https://doi.org/10.1016/j.foodchem.2022.132919" @default.
- W4223971878 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35421656" @default.
- W4223971878 hasPublicationYear "2022" @default.
- W4223971878 type Work @default.
- W4223971878 citedByCount "16" @default.
- W4223971878 countsByYear W42239718782022 @default.
- W4223971878 countsByYear W42239718782023 @default.
- W4223971878 crossrefType "journal-article" @default.
- W4223971878 hasAuthorship W4223971878A5027950443 @default.
- W4223971878 hasAuthorship W4223971878A5031837561 @default.
- W4223971878 hasAuthorship W4223971878A5035754451 @default.
- W4223971878 hasAuthorship W4223971878A5040827219 @default.
- W4223971878 hasAuthorship W4223971878A5045297186 @default.
- W4223971878 hasAuthorship W4223971878A5050018342 @default.
- W4223971878 hasAuthorship W4223971878A5053771367 @default.
- W4223971878 hasAuthorship W4223971878A5059281655 @default.
- W4223971878 hasAuthorship W4223971878A5082086115 @default.
- W4223971878 hasAuthorship W4223971878A5082346338 @default.
- W4223971878 hasConcept C119128265 @default.
- W4223971878 hasConcept C121332964 @default.
- W4223971878 hasConcept C161176658 @default.
- W4223971878 hasConcept C181199279 @default.
- W4223971878 hasConcept C185592680 @default.
- W4223971878 hasConcept C21951064 @default.
- W4223971878 hasConcept C2777028655 @default.
- W4223971878 hasConcept C2777207434 @default.
- W4223971878 hasConcept C2778359560 @default.
- W4223971878 hasConcept C2778617687 @default.
- W4223971878 hasConcept C2778816929 @default.
- W4223971878 hasConcept C2779059548 @default.
- W4223971878 hasConcept C2909509320 @default.
- W4223971878 hasConcept C43617362 @default.
- W4223971878 hasConcept C552990157 @default.
- W4223971878 hasConcept C55493867 @default.
- W4223971878 hasConcept C62520636 @default.
- W4223971878 hasConcept C6557445 @default.
- W4223971878 hasConcept C84211826 @default.
- W4223971878 hasConcept C86803240 @default.
- W4223971878 hasConcept C91881484 @default.
- W4223971878 hasConceptScore W4223971878C119128265 @default.
- W4223971878 hasConceptScore W4223971878C121332964 @default.
- W4223971878 hasConceptScore W4223971878C161176658 @default.
- W4223971878 hasConceptScore W4223971878C181199279 @default.
- W4223971878 hasConceptScore W4223971878C185592680 @default.
- W4223971878 hasConceptScore W4223971878C21951064 @default.
- W4223971878 hasConceptScore W4223971878C2777028655 @default.