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- W4223998532 abstract "Spermatogenesis is the process by which diploid male germ cells propagate and differentiate into haploid flagellated spermatozoa. This highly complicated process is dependent on testicular somatic cells maturation. While the role of these somatic cells in spermatogenesis is relatively well established, knowledge about their development and maturation, particularly at the molecular level, is limited. In this study, we profiled the testicular single-cell transcriptomes of Guanzhong black pigs at the age of 7, 30, 60, 90, and 150 days. Five types of Sertoli cells, five types of Leydig cells, and four types of peritubular myoid cells were identified. Histological analysis revealed the changes in proliferation levels and marker gene expressions, and the prion-like protein gene (PRND) was identified as a novel marker for Sertoli cells. Additionally, integrated analyses of porcine and human datasets revealed similarities between human and pig testicular somatic cells. Overall, the data obtained in this study contribute to the understanding of testicular development in pigs as a model species." @default.
- W4223998532 created "2022-04-19" @default.
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- W4223998532 date "2022-11-01" @default.
- W4223998532 modified "2023-10-17" @default.
- W4223998532 title "Single-cell RNA-seq analysis of testicular somatic cell development in pigs" @default.
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- W4223998532 doi "https://doi.org/10.1016/j.jgg.2022.03.014" @default.
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