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• W4224011785 abstract "Abstract Background Chlorhexidine gluconate (CHG) is a disinfectant agent with different applications in health care. Improper use of CHG causes antimicrobial resistance in bacteria as a public health threat. Since Staphylococcus aureus is a common bacteria, it is expected usually exposed to CHG in the hospital and community. The present study aimed to correlate the phenotypic and genotypic changes in a S. aureus strain upon serial adaptation with supra-inhibitory CHG concentration for 50 days. Results After in vitro serial culture of 5 × 10 5 CFU/ml of a clinical vancomycin-susceptible S. aureus strain (VAN-S) into brain heart infusion (BHI) broth containing CHG 1/4, 1/2, 1, and 2 × minimal inhibitory concentration (MIC) values of VAN-S in 37 °C during 50 days, we isolated a S. aureus strain (CHG Van-I ) with a ≥ twofold decrease in susceptibility to CHG and vancomycin. CHG-induced CHG Van-I strain was considered as a vancomycin-intermediate S. aureus (VISA) strain with a VAN MIC of 4 μg/ml using the broth macro dilution method. However, reduced resistance was observed to tetracycline family antibiotics (doxycycline and tetracycline) using a modified Kirby-Bauer disk diffusion test. Moreover, a remarkable reduction was detected in growth rate, hemolysis activity (the lysis of human red blood cells by alpha-hemolysin), and colony pigmentation (on BHI agar plates). Biofilm formation (using the Microtiter plate method and crystal violet staining) was significantly increased upon CHG treatment. Adaptive changes in the expression of a set of common genes related to the development of VISA phenotype ( gra TSR, vra TSR, wal KR, agr RNAIII, sce D, pbp B, and fmt A) were analyzed by Reverse Transcription quantitative PCR (RT-qPCR) experiment. Significant changes in vra TSR, agr RNAIII, sce D, and pbp B expression were observed. However, gene sequencing of the two-component system vra TSR using the Sanger sequencing method did not detect any non-synonymous substitution in CHG Van-I compared to wild-type. The clonality of VAN-S and CHG Van-I strains was verified using the pulsed-field gel electrophoresis (PFGE) method. Conclusions The importance of the present study should be stated in new detected mechanisms underlying VISA development. We found a link between the improper CHX use and the development of phenotypic and genotypic features, typical of VISA clinical isolates, in a CHG-induced strain. Since disruption of the cell wall biosynthesis occurs in VISA isolates, our CHG-induced VISA strain proved new insights into the role of CHG in the stimulation of the S. aureus cell wall." @default.
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• W4224011785 date "2022-04-13" @default.
• W4224011785 modified "2023-09-25" @default.
• W4224011785 title "Phenotypic and genotypic changes of Staphylococcus aureus in the presence of the inappropriate concentration of chlorhexidine gluconate" @default.
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• W4224011785 doi "https://doi.org/10.1186/s12866-022-02522-0" @default.
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