Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224016985> ?p ?o ?g. }
- W4224016985 abstract "ABSTRACT Background Cell groups containing catecholamines provide a useful model to study the molecular and cellular mechanisms underlying the morphogenesis, physiology, and pathology of the central nervous system. For this purpose, it is necessary to establish a system to induce catecholaminergic group-specific expression of Cre recombinase. Recently, we introduced a gene cassette encoding 2A peptide fused to Cre recombinase into the site between the C-terminus and translational termination codons of the rat tyrosine hydroxylase (TH) open reading frame by the Combi -CRISPR technology, which is a genomic editing method to enable an efficient knock-in (KI) of long DNA sequence into a target site. However, the expression patterns of the transgene and its function as well as the effect of the mutation on the biochemical and behavioral phenotypes in the KI strains have not been characterized yet. New Method We aimed to evaluate the usefulness of TH-Cre KI rats as an experimental model for investigating the structure and function of catecholaminergic neurons in the brain. Results We detected cell type-specific expression of Cre recombinase and site-specific recombination activity in the representative catecholaminergic groups in the TH-Cre KI rat strains. In addition, we measured TH expression level and catecholamine accumulation in the brain regions, and spontaneous locomotion, indicating that catecholamine metabolism and general behavior are apparently normal in these KI rats. Conclusions TH-Cre KI rat strains produced by the Combi -CRISPR system offer a beneficial model to study the molecular and cellular mechanics for the morphogenesis, physiology, and pathology of catecholamine-containing neurons in the brain." @default.
- W4224016985 created "2022-04-19" @default.
- W4224016985 creator A5018775199 @default.
- W4224016985 creator A5020663579 @default.
- W4224016985 creator A5022201721 @default.
- W4224016985 creator A5050833395 @default.
- W4224016985 creator A5055112378 @default.
- W4224016985 creator A5065319313 @default.
- W4224016985 creator A5065697763 @default.
- W4224016985 creator A5072781989 @default.
- W4224016985 creator A5089908182 @default.
- W4224016985 date "2022-04-13" @default.
- W4224016985 modified "2023-10-15" @default.
- W4224016985 title "Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the <i>Combi</i>-CRISPR technology" @default.
- W4224016985 cites W1091016337 @default.
- W4224016985 cites W1484258617 @default.
- W4224016985 cites W1509304378 @default.
- W4224016985 cites W1574147452 @default.
- W4224016985 cites W1745700293 @default.
- W4224016985 cites W1853268599 @default.
- W4224016985 cites W1968708464 @default.
- W4224016985 cites W1977658700 @default.
- W4224016985 cites W1987332330 @default.
- W4224016985 cites W1996502991 @default.
- W4224016985 cites W1997438162 @default.
- W4224016985 cites W2005452242 @default.
- W4224016985 cites W2015723327 @default.
- W4224016985 cites W2018164760 @default.
- W4224016985 cites W2018303025 @default.
- W4224016985 cites W2021043409 @default.
- W4224016985 cites W2023545713 @default.
- W4224016985 cites W2024626278 @default.
- W4224016985 cites W2027147867 @default.
- W4224016985 cites W2032086520 @default.
- W4224016985 cites W2035700730 @default.
- W4224016985 cites W2045452241 @default.
- W4224016985 cites W2056553920 @default.
- W4224016985 cites W2061338238 @default.
- W4224016985 cites W2064789218 @default.
- W4224016985 cites W2067147530 @default.
- W4224016985 cites W2069441590 @default.
- W4224016985 cites W2071609271 @default.
- W4224016985 cites W2080987963 @default.
- W4224016985 cites W2087917003 @default.
- W4224016985 cites W2125661818 @default.
- W4224016985 cites W2127508962 @default.
- W4224016985 cites W2140754284 @default.
- W4224016985 cites W2141410846 @default.
- W4224016985 cites W2161467344 @default.
- W4224016985 cites W2167279371 @default.
- W4224016985 cites W2169510202 @default.
- W4224016985 cites W2182650290 @default.
- W4224016985 cites W2215748303 @default.
- W4224016985 cites W2529445587 @default.
- W4224016985 cites W2614850398 @default.
- W4224016985 cites W2734600211 @default.
- W4224016985 cites W2787581258 @default.
- W4224016985 cites W2788203338 @default.
- W4224016985 cites W2931032935 @default.
- W4224016985 cites W3002904268 @default.
- W4224016985 cites W3004443958 @default.
- W4224016985 cites W3036190230 @default.
- W4224016985 cites W3039595652 @default.
- W4224016985 cites W3047624096 @default.
- W4224016985 cites W3096637692 @default.
- W4224016985 cites W3125589350 @default.
- W4224016985 cites W3126639184 @default.
- W4224016985 doi "https://doi.org/10.1101/2022.04.13.488137" @default.
- W4224016985 hasPublicationYear "2022" @default.
- W4224016985 type Work @default.
- W4224016985 citedByCount "0" @default.
- W4224016985 crossrefType "posted-content" @default.
- W4224016985 hasAuthorship W4224016985A5018775199 @default.
- W4224016985 hasAuthorship W4224016985A5020663579 @default.
- W4224016985 hasAuthorship W4224016985A5022201721 @default.
- W4224016985 hasAuthorship W4224016985A5050833395 @default.
- W4224016985 hasAuthorship W4224016985A5055112378 @default.
- W4224016985 hasAuthorship W4224016985A5065319313 @default.
- W4224016985 hasAuthorship W4224016985A5065697763 @default.
- W4224016985 hasAuthorship W4224016985A5072781989 @default.
- W4224016985 hasAuthorship W4224016985A5089908182 @default.
- W4224016985 hasBestOaLocation W42240169851 @default.
- W4224016985 hasConcept C102230213 @default.
- W4224016985 hasConcept C102414288 @default.
- W4224016985 hasConcept C104317684 @default.
- W4224016985 hasConcept C125929170 @default.
- W4224016985 hasConcept C141035611 @default.
- W4224016985 hasConcept C1491633281 @default.
- W4224016985 hasConcept C153911025 @default.
- W4224016985 hasConcept C156695909 @default.
- W4224016985 hasConcept C159167319 @default.
- W4224016985 hasConcept C169760540 @default.
- W4224016985 hasConcept C189014844 @default.
- W4224016985 hasConcept C2775894120 @default.
- W4224016985 hasConcept C2776824294 @default.
- W4224016985 hasConcept C2796436 @default.
- W4224016985 hasConcept C43601244 @default.
- W4224016985 hasConcept C513476851 @default.
- W4224016985 hasConcept C54355233 @default.
- W4224016985 hasConcept C86803240 @default.