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• W4224069789 abstract "Abstract Cryo-electron tomograms capture a wealth of structural information on the molecular constituents of cells and tissues. We present DeePiCt (Deep Picker in Context), an open-source deep-learning framework for supervised structure segmentation and macromolecular complex localization in cellular cryo-electron tomography. To train and benchmark DeePiCt on experimental data, we comprehensively annotated 20 tomograms of Schizosaccharomyces pombe for ribosomes, fatty acid synthases, membranes, nuclear pore complexes, organelles and cytosol. By comparing our method to state-of-the-art approaches on this dataset, we show its unique ability to identify low-abundance and low-density complexes. We use DeePiCt to study compositionally-distinct subpopulations of cellular ribosomes, with emphasis on their contextual association with mitochondria and the endoplasmic reticulum. Finally, by applying pre-trained networks to a HeLa cell dataset, we demonstrate that DeePiCt achieves high-quality predictions in unseen datasets from different biological species in a matter of minutes. The comprehensively annotated experimental data and pre-trained networks are provided for immediate exploitation by the community." @default.
• W4224069789 created "2022-04-19" @default.
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• W4224069789 date "2022-04-13" @default.
• W4224069789 modified "2023-10-15" @default.
• W4224069789 title "Convolutional networks for supervised mining of molecular patterns within cellular context" @default.
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• W4224069789 doi "https://doi.org/10.1101/2022.04.12.488077" @default.
• W4224069789 hasPublicationYear "2022" @default.

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