Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224126135> ?p ?o ?g. }
- W4224126135 endingPage "2967" @default.
- W4224126135 startingPage "2953" @default.
- W4224126135 abstract "Outer membrane lipoprotein A (OmlA) is a vaccine antigen against porcine contagious pleuropneumonia (PCP), a disease severely affecting the swine industry. Here, we aimed to systematically potentiate the secretory production of OmlA in Corynebacterium glutamicum (C. glutamicum), a widely used microorganism in the food industry, by establishing a holistic development process based on our high-throughput culture platform. The expression patterns, expression element combinations, medium composition, and induction conditions were comprehensively screened or optimized in microwell plates (MWPs), followed by fermentation parameter optimization in a 4 × 1 L parallel fermentation system (CUBER4). An unprecedented yield of 1.01 g/L OmlA was ultimately achieved in a 5-L bioreactor following the scaling-up strategy of fixed oxygen mass transfer coefficient (kLa), and the produced OmlA antigen showed well-protective immunity against Actinobacillus pleuropneumoniae challenge. This result provides a rapid and reliable pipeline to achieve the hyper-production of OmlA, and possibly other recombinant vaccines, in C. glutamicum. KEY POINTS: • Established a holistic development process and applied it to potentiate the secretion of OmlA. • The secretion of OmlA reached an unprecedented yield of 1.01 g/L. • The recombinant OmlA antigen induced efficient protective immunity." @default.
- W4224126135 created "2022-04-19" @default.
- W4224126135 creator A5005756384 @default.
- W4224126135 creator A5008015122 @default.
- W4224126135 creator A5011675587 @default.
- W4224126135 creator A5014867897 @default.
- W4224126135 creator A5017682791 @default.
- W4224126135 creator A5020473054 @default.
- W4224126135 creator A5025431883 @default.
- W4224126135 creator A5027760785 @default.
- W4224126135 creator A5046280013 @default.
- W4224126135 creator A5046819071 @default.
- W4224126135 date "2022-04-01" @default.
- W4224126135 modified "2023-10-17" @default.
- W4224126135 title "Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process" @default.
- W4224126135 cites W1669778109 @default.
- W4224126135 cites W1976189088 @default.
- W4224126135 cites W1980780067 @default.
- W4224126135 cites W1991451972 @default.
- W4224126135 cites W1996734160 @default.
- W4224126135 cites W1999441390 @default.
- W4224126135 cites W2001426985 @default.
- W4224126135 cites W2009796811 @default.
- W4224126135 cites W2015833555 @default.
- W4224126135 cites W2018718171 @default.
- W4224126135 cites W2032078821 @default.
- W4224126135 cites W2033229084 @default.
- W4224126135 cites W2043381820 @default.
- W4224126135 cites W2050724684 @default.
- W4224126135 cites W2059645571 @default.
- W4224126135 cites W2064321998 @default.
- W4224126135 cites W2067572214 @default.
- W4224126135 cites W2069574883 @default.
- W4224126135 cites W2070388868 @default.
- W4224126135 cites W2071174323 @default.
- W4224126135 cites W2076503830 @default.
- W4224126135 cites W2081983234 @default.
- W4224126135 cites W2082719373 @default.
- W4224126135 cites W2085428764 @default.
- W4224126135 cites W2093178286 @default.
- W4224126135 cites W2093592006 @default.
- W4224126135 cites W2101591208 @default.
- W4224126135 cites W2102136421 @default.
- W4224126135 cites W2107767084 @default.
- W4224126135 cites W2116904877 @default.
- W4224126135 cites W2123039170 @default.
- W4224126135 cites W2133587231 @default.
- W4224126135 cites W2138820253 @default.
- W4224126135 cites W2140373206 @default.
- W4224126135 cites W2309786286 @default.
- W4224126135 cites W2537956729 @default.
- W4224126135 cites W2590981297 @default.
- W4224126135 cites W2734651258 @default.
- W4224126135 cites W2785993273 @default.
- W4224126135 cites W2792188161 @default.
- W4224126135 cites W2889550166 @default.
- W4224126135 cites W2898169606 @default.
- W4224126135 cites W2898576133 @default.
- W4224126135 cites W2945203504 @default.
- W4224126135 cites W3080494770 @default.
- W4224126135 cites W3081418767 @default.
- W4224126135 cites W3183444345 @default.
- W4224126135 cites W3202309656 @default.
- W4224126135 cites W4230079893 @default.
- W4224126135 cites W4232406365 @default.
- W4224126135 doi "https://doi.org/10.1007/s00253-022-11918-x" @default.
- W4224126135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35435456" @default.
- W4224126135 hasPublicationYear "2022" @default.
- W4224126135 type Work @default.
- W4224126135 citedByCount "3" @default.
- W4224126135 countsByYear W42241261352022 @default.
- W4224126135 countsByYear W42241261352023 @default.
- W4224126135 crossrefType "journal-article" @default.
- W4224126135 hasAuthorship W4224126135A5005756384 @default.
- W4224126135 hasAuthorship W4224126135A5008015122 @default.
- W4224126135 hasAuthorship W4224126135A5011675587 @default.
- W4224126135 hasAuthorship W4224126135A5014867897 @default.
- W4224126135 hasAuthorship W4224126135A5017682791 @default.
- W4224126135 hasAuthorship W4224126135A5020473054 @default.
- W4224126135 hasAuthorship W4224126135A5025431883 @default.
- W4224126135 hasAuthorship W4224126135A5027760785 @default.
- W4224126135 hasAuthorship W4224126135A5046280013 @default.
- W4224126135 hasAuthorship W4224126135A5046819071 @default.
- W4224126135 hasBestOaLocation W42241261352 @default.
- W4224126135 hasConcept C100544194 @default.
- W4224126135 hasConcept C10389963 @default.
- W4224126135 hasConcept C104317684 @default.
- W4224126135 hasConcept C147483822 @default.
- W4224126135 hasConcept C168170006 @default.
- W4224126135 hasConcept C185592680 @default.
- W4224126135 hasConcept C203014093 @default.
- W4224126135 hasConcept C2776260651 @default.
- W4224126135 hasConcept C2780861368 @default.
- W4224126135 hasConcept C40767141 @default.
- W4224126135 hasConcept C49039625 @default.
- W4224126135 hasConcept C523546767 @default.
- W4224126135 hasConcept C54355233 @default.
- W4224126135 hasConcept C55493867 @default.