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- W4224214874 abstract "Abstract Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) pandemic has been considered with great importance on correct screening procedure. The detection efficiency of recent variants of concern were observed by comparing 5 commercial RT-PCR kits and a SYBR-green method developed and validated in our laboratory. The RNA was extracted from nasopharyngeal samples from suspected COVID-19 patients as instructed by each manufacturer and RT-PCR was also done according to standard procedures according to manufacturers. We found that commercially available Maccura kit (sensitivity and specificity > 80%), TaqPath kit (sensitivity and specificity > 80%) and in house SYBR-Green method (sensitivity and specificity > 90%) showed a consistent detection result. We also found that detection kits targeting more genes show a better result than less target gene containing protocols which facilitates less false positive results (< 20%) and better diagnostic accuracy (> 80%). Our study also showed a significant difference (sig. <0.005) in Ct value reported for common target genes shared by the RT-PCR kits in relation with different variants, specifically the Variants of Concerns (VOCs) tends to report a higher Ct value than wild type SARS-CoV-2 and suggests to detect with more target genes for better accuracy and better assessment of COVID-19 infection." @default.
- W4224214874 created "2022-04-26" @default.
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- W4224214874 date "2022-04-21" @default.
- W4224214874 modified "2023-10-18" @default.
- W4224214874 title "Evaluation of RT-PCR assays for detection of SARS-CoV-2 variants of concern." @default.
- W4224214874 doi "https://doi.org/10.21203/rs.3.rs-1476789/v1" @default.
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