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- W4224242221 endingPage "101965" @default.
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- W4224242221 abstract "Genetic technology using site-specific recombinases, such as the Cre-loxP system, has been widely employed for labeling specific cell populations and for studying their functions in vivo. To enhance the precision of cell lineage tracing and functional study, a similar site-specific recombinase system termed Dre-rox has been recently used in combination with Cre-loxP. To enable more specific cell lineage tracing and ablation through dual recombinase activity, we generated two mouse lines that render Dre- or Dre+Cre-mediated recombination to excise a stop codon sequence that prevents the expression of diphtheria toxin receptor (DTR) knocked into the ubiquitously expressed and safe Rosa26 locus. Using different Dre- and Cre-expressing mouse lines, we showed that the surrogate gene reporters tdTomato and DTR were simultaneously expressed in target cells and in their descendants, and we observed efficient ablation of tdTomato+ cells after diphtheria toxin administration. These mouse lines were used to simultaneously trace and deplete the target cells of interest through the inducible expression of a reporter and DTR using dual Cre and Dre recombinases, allowing a more precise and efficient study of the role of specific cell subsets within a heterogeneous population in pathophysiological conditions in vivo." @default.
- W4224242221 created "2022-04-26" @default.
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- W4224242221 date "2022-06-01" @default.
- W4224242221 modified "2023-10-05" @default.
- W4224242221 title "Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo" @default.
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- W4224242221 doi "https://doi.org/10.1016/j.jbc.2022.101965" @default.
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