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- W4224252898 abstract "Abstract Skin is an active immune organ where professional antigen-presenting cells such as epidermal Langerhans cells (LCs) link innate and adaptive immune responses. While Reticulon 1A (RTN1A) was recently identified in LCs and dendritic cells in cutaneous and lymphoid tissues of humans and mice, its function is still unclear. Here, we studied the involvement of this protein in cytoskeletal remodeling and immune responses towards pathogens by stimulation of Toll-like receptors (TLRs) in resident LCs (rLCs) and emigrated LCs (eLCs) in human epidermis ex vivo and in a transgenic THP-1 RTN1A + cell line. Hampering RTN1A functionality through an inhibitory antibody induced significant dendrite retraction of rLCs and inhibited their emigration. Similarly, expression of RTN1A in THP-1 cells significantly altered their morphology, enhanced aggregation potential and inhibited the Ca 2+ flux. Differentiated THP-1 RTN1A + macrophages exhibited long cell protrusions and a larger cell body size in comparison to wild type cells. Further, stimulation of epidermal sheets with bacterial lipoproteins (TLR1/2 and TLR2) and single-stranded RNA (TLR7) resulted in the formation of substantial clusters of rLCs and a significant decrease of RTN1A expression in eLCs. Together, our data indicate involvement of RTN1A in dendrite dynamics and structural plasticity of primary LCs. Moreover, we discovered a relation between activation of TLRs, clustering of LCs and downregulation of RTN1A within the epidermis, thus indicating an important role of RTN1A in LC residency and maintaining tissue homeostasis. Graphical abstract Highlights Blocking of RTN1A induces dendrite retraction of resident LCs (rLCs) in epidermal explants. Despite a roundish morphology rLCs exhibit reduced migration capacity. RTN1A has an inhibitory effect on the calcium flux. Toll-like receptor-activated rLCs form vast clusters and significantly diminish RTN1A expression after emigration. RTN1A plays a central role in LC residency and maintaining tissue homeostasis." @default.
- W4224252898 created "2022-04-26" @default.
- W4224252898 creator A5021114812 @default.
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- W4224252898 creator A5090151321 @default.
- W4224252898 date "2022-04-08" @default.
- W4224252898 modified "2023-09-28" @default.
- W4224252898 title "Interoperability of RTN1A in dendrite dynamics and immune functions in human Langerhans cells" @default.
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- W4224252898 doi "https://doi.org/10.1101/2022.04.08.487626" @default.
- W4224252898 hasPublicationYear "2022" @default.
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