FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224253760> ?p ?o ?g. }

• W4224253760 endingPage "2451" @default.
• W4224253760 startingPage "2443" @default.
• W4224253760 abstract "Predictable DNA off-target effect is one of the major safety concerns for the application of cytosine base editors (CBEs). To eliminate Cas9-dependent DNA off-target effects, we designed a novel effective CBE system with dual guiders by combining CRISPR with transcription activator-like effector (TALE). In this system, Cas9 nickase (nCas9) and cytosine deaminase are guided to the same target site to conduct base editing by single-guide RNA (sgRNA) and TALE, respectively. However, if nCas9 is guided to a wrong site by sgRNA, it will not generate base editing due to the absence of deaminase. Similarly, when deaminase is guided to a wrong site by TALE, base editing will not occur due to the absence of single-stranded DNA. In this way, Cas9- and TALE-dependent DNA off-target effects could be completely eliminated. Furthermore, by fusing TALE with YE1, a cytidine deaminase with minimal Cas9-independent off-target effect, we established a novel CBE that could induce efficient C-to-T conversion without detectable Cas9- or TALE-dependent DNA off-target mutations. Predictable DNA off-target effect is one of the major safety concerns for the application of cytosine base editors (CBEs). To eliminate Cas9-dependent DNA off-target effects, we designed a novel effective CBE system with dual guiders by combining CRISPR with transcription activator-like effector (TALE). In this system, Cas9 nickase (nCas9) and cytosine deaminase are guided to the same target site to conduct base editing by single-guide RNA (sgRNA) and TALE, respectively. However, if nCas9 is guided to a wrong site by sgRNA, it will not generate base editing due to the absence of deaminase. Similarly, when deaminase is guided to a wrong site by TALE, base editing will not occur due to the absence of single-stranded DNA. In this way, Cas9- and TALE-dependent DNA off-target effects could be completely eliminated. Furthermore, by fusing TALE with YE1, a cytidine deaminase with minimal Cas9-independent off-target effect, we established a novel CBE that could induce efficient C-to-T conversion without detectable Cas9- or TALE-dependent DNA off-target mutations." @default.
• W4224253760 created "2022-04-26" @default.
• W4224253760 creator A5012718640 @default.
• W4224253760 creator A5016038041 @default.
• W4224253760 creator A5018073672 @default.
• W4224253760 creator A5020040404 @default.
• W4224253760 creator A5021979515 @default.
• W4224253760 creator A5023363049 @default.
• W4224253760 creator A5025441318 @default.
• W4224253760 creator A5036898919 @default.
• W4224253760 creator A5049692788 @default.
• W4224253760 creator A5052341805 @default.
• W4224253760 creator A5053149886 @default.
• W4224253760 creator A5054718885 @default.
• W4224253760 creator A5056101402 @default.
• W4224253760 creator A5075318484 @default.
• W4224253760 creator A5082870343 @default.
• W4224253760 creator A5085415365 @default.
• W4224253760 creator A5088060299 @default.
• W4224253760 creator A5088173549 @default.
• W4224253760 creator A5088761700 @default.
• W4224253760 creator A5089082135 @default.
• W4224253760 date "2022-07-01" @default.
• W4224253760 modified "2023-10-18" @default.
• W4224253760 title "Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE" @default.
• W4224253760 cites W1857963905 @default.
• W4224253760 cites W1988967913 @default.
• W4224253760 cites W2025074364 @default.
• W4224253760 cites W2040698316 @default.
• W4224253760 cites W2114838239 @default.
• W4224253760 cites W2115603949 @default.
• W4224253760 cites W2129194497 @default.
• W4224253760 cites W2149402050 @default.
• W4224253760 cites W2336828812 @default.
• W4224253760 cites W2548327551 @default.
• W4224253760 cites W2621647941 @default.
• W4224253760 cites W2766599608 @default.
• W4224253760 cites W2793309516 @default.
• W4224253760 cites W2809042973 @default.
• W4224253760 cites W2873109489 @default.
• W4224253760 cites W2916364858 @default.
• W4224253760 cites W2917855738 @default.
• W4224253760 cites W2939749480 @default.
• W4224253760 cites W2951233129 @default.
• W4224253760 cites W2954739215 @default.
• W4224253760 cites W3000070051 @default.
• W4224253760 cites W3000375097 @default.
• W4224253760 cites W3004494456 @default.
• W4224253760 cites W3025828224 @default.
• W4224253760 cites W3041877641 @default.
• W4224253760 cites W3043143974 @default.
• W4224253760 cites W3043719325 @default.
• W4224253760 cites W3044027016 @default.
• W4224253760 cites W3082199632 @default.
• W4224253760 cites W3160764929 @default.
• W4224253760 cites W3197248033 @default.
• W4224253760 cites W3198162943 @default.
• W4224253760 cites W3207266761 @default.
• W4224253760 cites W3208207164 @default.
• W4224253760 cites W3215850915 @default.
• W4224253760 doi "https://doi.org/10.1016/j.ymthe.2022.04.010" @default.
• W4224253760 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35443934" @default.
• W4224253760 hasPublicationYear "2022" @default.
• W4224253760 type Work @default.
• W4224253760 citedByCount "7" @default.
• W4224253760 countsByYear W42242537602022 @default.
• W4224253760 countsByYear W42242537602023 @default.
• W4224253760 crossrefType "journal-article" @default.
• W4224253760 hasAuthorship W4224253760A5012718640 @default.
• W4224253760 hasAuthorship W4224253760A5016038041 @default.
• W4224253760 hasAuthorship W4224253760A5018073672 @default.
• W4224253760 hasAuthorship W4224253760A5020040404 @default.
• W4224253760 hasAuthorship W4224253760A5021979515 @default.
• W4224253760 hasAuthorship W4224253760A5023363049 @default.
• W4224253760 hasAuthorship W4224253760A5025441318 @default.
• W4224253760 hasAuthorship W4224253760A5036898919 @default.
• W4224253760 hasAuthorship W4224253760A5049692788 @default.
• W4224253760 hasAuthorship W4224253760A5052341805 @default.
• W4224253760 hasAuthorship W4224253760A5053149886 @default.
• W4224253760 hasAuthorship W4224253760A5054718885 @default.
• W4224253760 hasAuthorship W4224253760A5056101402 @default.
• W4224253760 hasAuthorship W4224253760A5075318484 @default.
• W4224253760 hasAuthorship W4224253760A5082870343 @default.
• W4224253760 hasAuthorship W4224253760A5085415365 @default.
• W4224253760 hasAuthorship W4224253760A5088060299 @default.
• W4224253760 hasAuthorship W4224253760A5088173549 @default.
• W4224253760 hasAuthorship W4224253760A5088761700 @default.
• W4224253760 hasAuthorship W4224253760A5089082135 @default.
• W4224253760 hasBestOaLocation W42242537601 @default.
• W4224253760 hasConcept C104317684 @default.
• W4224253760 hasConcept C111599444 @default.
• W4224253760 hasConcept C128526571 @default.
• W4224253760 hasConcept C132455925 @default.
• W4224253760 hasConcept C144501496 @default.
• W4224253760 hasConcept C159654299 @default.
• W4224253760 hasConcept C27082348 @default.
• W4224253760 hasConcept C2776023528 @default.
• W4224253760 hasConcept C2776985229 @default.

• next