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- W4224254871 endingPage "3839" @default.
- W4224254871 startingPage "3829" @default.
- W4224254871 abstract "Summary Metabarcoding approaches are exponentially increasing our understanding of soil biodiversity, with a major focus on the bacterial part of the microbiome. Part of the soil diversity are also eukaryotes that include fungi, algae, protists and Metazoa. Nowadays, soil eukaryotes are targeted with the same approaches developed for bacteria and archaea (prokaryotes). However, fundamental differences exist between domains. After providing a short historical overview of the developments of metabarcoding applied to environmental microbiology, we compile the most important differences between domains that prevent direct method transfers between prokaryotic and eukaryotic soil metabarcoding approaches, currently dominated by short‐read sequencing. These include the existence of divergent diversity concepts and the variations in eukaryotic morphology that affect sampling and DNA extraction. Furthermore, eukaryotes experienced much more variable evolutionary rates than prokaryotes, which prevent capturing the entire eukaryotic diversity in a soil with a single amplification protocol fit for short‐read sequencing. In the final part we focus on future potentials for optimization of eukaryotic metabarcoding that include superior possibility of functionally characterizing eukaryotes and to extend the current information obtained, such as by adding a real quantitative component. This review should optimize future metabarcoding approaches targeting soil eukaryotes and kickstart this promising research direction." @default.
- W4224254871 created "2022-04-26" @default.
- W4224254871 creator A5012357264 @default.
- W4224254871 creator A5040233192 @default.
- W4224254871 creator A5065196681 @default.
- W4224254871 date "2022-04-24" @default.
- W4224254871 modified "2023-10-10" @default.
- W4224254871 title "Discrepancies between prokaryotes and eukaryotes need to be considered in soil <scp>DNA</scp>‐based studies" @default.
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