Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224256677> ?p ?o ?g. }
- W4224256677 endingPage "368" @default.
- W4224256677 startingPage "359" @default.
- W4224256677 abstract "Abstract Porcupine (Porcn) enzyme plays an essential role in Wnt signaling activation. Stearoyl‐CoA desaturase‐1 (SCD1) is required to provide Porcn substrates. The aim of this study was to determine the effect of a novel Porcn inhibitor on the fate of human embryonic stem cells (hESCs) and the reliance of Porcn on SCD1 activity. hESCs were cultured on a feeder layer or Matrigel‐coated plates. Small molecules WNT974 (LGK‐974) and CAY10566 were used to inhibit Porcn and SCD1 activity, respectively. We assessed the effect of Porcn inhibition on viability, expression of Wnt signaling targets, pluripotency markers, proliferation, differentiation, and protein fatty acylation. hESCs' conditioned medium (CM) containing secreted Wnt proteins were applied in rescue experiments. To examine the catalytic dependency of Porcn on SCD1, the results of combined inhibitor treatment were compared with the SCD1 inhibitor alone. LGK‐974 at the selected concentrations showed mild effects on hESCs viability, but significantly reduced messenger RNA and protein expression of Wnt signaling targets (Axin‐2 and c‐Myc) and pluripotency markers (OCT‐4 and SOX‐2) ( p < .05). Adding 1 μM of Porcn inhibitor reduced proliferation ( p = .03) and enhanced differentiation capacity into ectodermal progenitors ( p = .02), which were reverted by CM. Click chemistry reaction did not show significant alteration in protein fatty acylation upon LGK‐974 treatment. Moreover, combined inhibitor treatment caused no further substantial reduction in Wnt signaling targets, pluripotency markers, and protein fatty acylation relative to CAY10566‐treated cultures. The substrate availability for Porcn activity is regulated by SCD1 and targeting Porcn by LGK‐974 prompts the transition of hESCs from self‐renewal state to ectodermal lineage." @default.
- W4224256677 created "2022-04-26" @default.
- W4224256677 creator A5000946437 @default.
- W4224256677 creator A5008945537 @default.
- W4224256677 creator A5019064712 @default.
- W4224256677 creator A5022565845 @default.
- W4224256677 creator A5030049548 @default.
- W4224256677 creator A5053815765 @default.
- W4224256677 creator A5081484085 @default.
- W4224256677 creator A5089523796 @default.
- W4224256677 date "2022-04-21" @default.
- W4224256677 modified "2023-09-27" @default.
- W4224256677 title "The porcupine inhibitor WNT974 provokes ectodermal lineage differentiation of human embryonic stem cells" @default.
- W4224256677 cites W1693243095 @default.
- W4224256677 cites W1928317628 @default.
- W4224256677 cites W1980904258 @default.
- W4224256677 cites W1987833134 @default.
- W4224256677 cites W1987966039 @default.
- W4224256677 cites W1994029539 @default.
- W4224256677 cites W2000600409 @default.
- W4224256677 cites W2018744859 @default.
- W4224256677 cites W2044249881 @default.
- W4224256677 cites W2051859926 @default.
- W4224256677 cites W2057982628 @default.
- W4224256677 cites W2067056027 @default.
- W4224256677 cites W2070427174 @default.
- W4224256677 cites W2083599207 @default.
- W4224256677 cites W2106806803 @default.
- W4224256677 cites W2135068619 @default.
- W4224256677 cites W2139945482 @default.
- W4224256677 cites W2148392331 @default.
- W4224256677 cites W2166178545 @default.
- W4224256677 cites W2192364447 @default.
- W4224256677 cites W2238529573 @default.
- W4224256677 cites W2267953463 @default.
- W4224256677 cites W2290735351 @default.
- W4224256677 cites W2444554353 @default.
- W4224256677 cites W2563829784 @default.
- W4224256677 cites W2566405543 @default.
- W4224256677 cites W2593181149 @default.
- W4224256677 cites W2612678024 @default.
- W4224256677 cites W2621361823 @default.
- W4224256677 cites W2736850857 @default.
- W4224256677 cites W2766393670 @default.
- W4224256677 cites W2787119915 @default.
- W4224256677 cites W2897605243 @default.
- W4224256677 cites W2902964784 @default.
- W4224256677 cites W2903818334 @default.
- W4224256677 cites W2913780686 @default.
- W4224256677 cites W2917504273 @default.
- W4224256677 cites W2950675080 @default.
- W4224256677 cites W2977441471 @default.
- W4224256677 cites W3024405865 @default.
- W4224256677 cites W3097368743 @default.
- W4224256677 cites W4200445985 @default.
- W4224256677 doi "https://doi.org/10.1002/cbf.3700" @default.
- W4224256677 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35445405" @default.
- W4224256677 hasPublicationYear "2022" @default.
- W4224256677 type Work @default.
- W4224256677 citedByCount "2" @default.
- W4224256677 countsByYear W42242566772023 @default.
- W4224256677 crossrefType "journal-article" @default.
- W4224256677 hasAuthorship W4224256677A5000946437 @default.
- W4224256677 hasAuthorship W4224256677A5008945537 @default.
- W4224256677 hasAuthorship W4224256677A5019064712 @default.
- W4224256677 hasAuthorship W4224256677A5022565845 @default.
- W4224256677 hasAuthorship W4224256677A5030049548 @default.
- W4224256677 hasAuthorship W4224256677A5053815765 @default.
- W4224256677 hasAuthorship W4224256677A5081484085 @default.
- W4224256677 hasAuthorship W4224256677A5089523796 @default.
- W4224256677 hasConcept C104317684 @default.
- W4224256677 hasConcept C137620995 @default.
- W4224256677 hasConcept C145103041 @default.
- W4224256677 hasConcept C185592680 @default.
- W4224256677 hasConcept C55493867 @default.
- W4224256677 hasConcept C62478195 @default.
- W4224256677 hasConcept C86803240 @default.
- W4224256677 hasConcept C95444343 @default.
- W4224256677 hasConceptScore W4224256677C104317684 @default.
- W4224256677 hasConceptScore W4224256677C137620995 @default.
- W4224256677 hasConceptScore W4224256677C145103041 @default.
- W4224256677 hasConceptScore W4224256677C185592680 @default.
- W4224256677 hasConceptScore W4224256677C55493867 @default.
- W4224256677 hasConceptScore W4224256677C62478195 @default.
- W4224256677 hasConceptScore W4224256677C86803240 @default.
- W4224256677 hasConceptScore W4224256677C95444343 @default.
- W4224256677 hasIssue "4" @default.
- W4224256677 hasLocation W42242566771 @default.
- W4224256677 hasLocation W42242566772 @default.
- W4224256677 hasOpenAccess W4224256677 @default.
- W4224256677 hasPrimaryLocation W42242566771 @default.
- W4224256677 hasRelatedWork W1759652240 @default.
- W4224256677 hasRelatedWork W2008014566 @default.
- W4224256677 hasRelatedWork W2078337444 @default.
- W4224256677 hasRelatedWork W2123037811 @default.
- W4224256677 hasRelatedWork W2370215344 @default.
- W4224256677 hasRelatedWork W2782680922 @default.
- W4224256677 hasRelatedWork W2790748761 @default.