FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224267941> ?p ?o ?g. }

• W4224267941 endingPage "100" @default.
• W4224267941 startingPage "92" @default.
• W4224267941 abstract "The aim of this work is to develop a 3D cell culture model based on cell spheroids for predicting the functional activity of various compounds in vivo. Agarose gel molds were made using 3D printing. The solidified agarose gel is a matrix consisting of nine low-adhesive U-shaped microwells of 2.3 × 3.3 mm for 3D cell spheroid formation and growth. This matrix is placed into a single well of a 12-well plate. The effectiveness of the cell culture method was demonstrated using human ovarian carcinoma SKOVip-kat cells stably expressing the red fluorescent protein Katushka in the cytoplasm and overexpressing the membrane-associated tumor marker HER2. The SKOVip-kat cell spheroids were visualized by fluorescence microscopy. The cell concentration required for the formation of same-shape and same-size spheroids with tight intercellular contacts was optimized. To verify the developed model, the cytotoxicity of the targeted immunotoxin anti-HER2 consisting of the anti-HER2 scaffold DARP 9_29 and a fragment of the Pseudomonas aeroginosa exotoxin, DARP-LoPE, was studied in 2D and 3D SKOVip-kat cell cultures. The existence of a difference in the cytotoxic properties of DARP-LoPE between the 2D and 3D cultures has been demonstrated: the IC50 value in the 3D culture is an order of magnitude higher than that in the monolayer culture. The present work describes a universal tool for 3D cultivation of mammalian cells based on reusable agarose gel molds that allows for reproducible formation of multicellular spheroids with tight contacts for molecular and cell biology studies." @default.
• W4224267941 created "2022-04-26" @default.
• W4224267941 creator A5021908338 @default.
• W4224267941 creator A5033135435 @default.
• W4224267941 creator A5042643687 @default.
• W4224267941 creator A5055501415 @default.
• W4224267941 creator A5058335791 @default.
• W4224267941 creator A5069533915 @default.
• W4224267941 date "2022-05-10" @default.
• W4224267941 modified "2023-09-27" @default.
• W4224267941 title "3D Models of Cellular Spheroids As a Universal Tool for Studying the Cytotoxic Properties of Anticancer Compounds In Vitro" @default.
• W4224267941 cites W1725458096 @default.
• W4224267941 cites W1924899380 @default.
• W4224267941 cites W1966398068 @default.
• W4224267941 cites W1976329194 @default.
• W4224267941 cites W1979129905 @default.
• W4224267941 cites W1981059065 @default.
• W4224267941 cites W1992875306 @default.
• W4224267941 cites W2030185565 @default.
• W4224267941 cites W2048644480 @default.
• W4224267941 cites W2055699288 @default.
• W4224267941 cites W2055792279 @default.
• W4224267941 cites W2056839334 @default.
• W4224267941 cites W2069707480 @default.
• W4224267941 cites W2078454159 @default.
• W4224267941 cites W2083053655 @default.
• W4224267941 cites W2106443011 @default.
• W4224267941 cites W2114918609 @default.
• W4224267941 cites W2120043042 @default.
• W4224267941 cites W2134481788 @default.
• W4224267941 cites W2165238581 @default.
• W4224267941 cites W2236643502 @default.
• W4224267941 cites W2288763182 @default.
• W4224267941 cites W2315439481 @default.
• W4224267941 cites W2324898974 @default.
• W4224267941 cites W2338693687 @default.
• W4224267941 cites W2468971141 @default.
• W4224267941 cites W2474641201 @default.
• W4224267941 cites W2587788572 @default.
• W4224267941 cites W2599138920 @default.
• W4224267941 cites W2606980922 @default.
• W4224267941 cites W2743626936 @default.
• W4224267941 cites W2772921661 @default.
• W4224267941 cites W2943423304 @default.
• W4224267941 cites W2946178465 @default.
• W4224267941 cites W2989951920 @default.
• W4224267941 cites W3009670898 @default.
• W4224267941 cites W3037744682 @default.
• W4224267941 cites W3042085091 @default.
• W4224267941 cites W3043807509 @default.
• W4224267941 cites W3049331511 @default.
• W4224267941 cites W3082990138 @default.
• W4224267941 cites W3083803591 @default.
• W4224267941 cites W3093252756 @default.
• W4224267941 cites W3109866681 @default.
• W4224267941 cites W3112850117 @default.
• W4224267941 cites W3164359321 @default.
• W4224267941 cites W3168483671 @default.
• W4224267941 cites W3176581805 @default.
• W4224267941 cites W4200413970 @default.
• W4224267941 cites W4224242026 @default.
• W4224267941 cites W4252812826 @default.
• W4224267941 doi "https://doi.org/10.32607/actanaturae.11603" @default.
• W4224267941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35441052" @default.
• W4224267941 hasPublicationYear "2022" @default.
• W4224267941 type Work @default.
• W4224267941 citedByCount "5" @default.
• W4224267941 countsByYear W42242679412022 @default.
• W4224267941 countsByYear W42242679412023 @default.
• W4224267941 crossrefType "journal-article" @default.
• W4224267941 hasAuthorship W4224267941A5021908338 @default.
• W4224267941 hasAuthorship W4224267941A5033135435 @default.
• W4224267941 hasAuthorship W4224267941A5042643687 @default.
• W4224267941 hasAuthorship W4224267941A5055501415 @default.
• W4224267941 hasAuthorship W4224267941A5058335791 @default.
• W4224267941 hasAuthorship W4224267941A5069533915 @default.
• W4224267941 hasBestOaLocation W42242679411 @default.
• W4224267941 hasConcept C109316439 @default.
• W4224267941 hasConcept C121332964 @default.
• W4224267941 hasConcept C12554922 @default.
• W4224267941 hasConcept C1491633281 @default.
• W4224267941 hasConcept C150903083 @default.
• W4224267941 hasConcept C153911025 @default.
• W4224267941 hasConcept C154317977 @default.
• W4224267941 hasConcept C163688615 @default.
• W4224267941 hasConcept C169274487 @default.
• W4224267941 hasConcept C175369904 @default.
• W4224267941 hasConcept C185592680 @default.
• W4224267941 hasConcept C202751555 @default.
• W4224267941 hasConcept C207001950 @default.
• W4224267941 hasConcept C2778476535 @default.
• W4224267941 hasConcept C54355233 @default.
• W4224267941 hasConcept C55493867 @default.
• W4224267941 hasConcept C62520636 @default.
• W4224267941 hasConcept C81885089 @default.
• W4224267941 hasConcept C86803240 @default.
• W4224267941 hasConcept C91881484 @default.
• W4224267941 hasConcept C95444343 @default.

• next