FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224274981> ?p ?o ?g. }

• W4224274981 endingPage "1006" @default.
• W4224274981 startingPage "996" @default.
• W4224274981 abstract "We report the development of a high-throughput, intracellular transcription block survival (TBS) screening platform to derive functional transcription factor antagonists. TBS is demonstrated using the oncogenic transcriptional regulator cJun, with the development of antagonists that bind cJun and prevent both dimerization and, more importantly, DNA binding remaining a primary challenge. In TBS, cognate TRE sites are introduced into the coding region of the essential gene, dihydrofolate reductase (DHFR). Introduction of cJun leads to TRE binding, preventing DHFR expression by directly blocking RNA polymerase gene transcription to abrogate cell proliferation. Peptide library screening identified a sequence that both binds cJun and antagonizes function by preventing DNA binding, as demonstrated by restored cell viability and subsequent in vitro hit validation. TBS is an entirely tag-free genotype-to-phenotype approach, selecting desirable attributes such as high solubility, target specificity, and low toxicity within a complex cellular environment. TBS facilitates rapid library screening to accelerate the identification of therapeutically valuable sequences." @default.
• W4224274981 created "2022-04-26" @default.
• W4224274981 creator A5001520016 @default.
• W4224274981 creator A5004541105 @default.
• W4224274981 creator A5007102926 @default.
• W4224274981 creator A5075473900 @default.
• W4224274981 date "2022-04-06" @default.
• W4224274981 modified "2023-10-18" @default.
• W4224274981 title "An Approach to Derive Functional Peptide Inhibitors of Transcription Factor Activity" @default.
• W4224274981 cites W117652395 @default.
• W4224274981 cites W1575004573 @default.
• W4224274981 cites W1576579253 @default.
• W4224274981 cites W1600616201 @default.
• W4224274981 cites W1860382351 @default.
• W4224274981 cites W1959523209 @default.
• W4224274981 cites W1978465076 @default.
• W4224274981 cites W1980864900 @default.
• W4224274981 cites W1994219871 @default.
• W4224274981 cites W2001900040 @default.
• W4224274981 cites W2002992087 @default.
• W4224274981 cites W2005293003 @default.
• W4224274981 cites W2014109226 @default.
• W4224274981 cites W2016686171 @default.
• W4224274981 cites W2016774526 @default.
• W4224274981 cites W2020081493 @default.
• W4224274981 cites W2039478144 @default.
• W4224274981 cites W2059251164 @default.
• W4224274981 cites W2063079196 @default.
• W4224274981 cites W2066072183 @default.
• W4224274981 cites W2072319964 @default.
• W4224274981 cites W2082367861 @default.
• W4224274981 cites W2083939457 @default.
• W4224274981 cites W2087640316 @default.
• W4224274981 cites W2092008649 @default.
• W4224274981 cites W2096506888 @default.
• W4224274981 cites W2097210639 @default.
• W4224274981 cites W2109319472 @default.
• W4224274981 cites W2120165202 @default.
• W4224274981 cites W2137465688 @default.
• W4224274981 cites W2147452955 @default.
• W4224274981 cites W2152766457 @default.
• W4224274981 cites W2238978610 @default.
• W4224274981 cites W2322523258 @default.
• W4224274981 cites W2329404090 @default.
• W4224274981 cites W2587458263 @default.
• W4224274981 cites W2753139186 @default.
• W4224274981 cites W2766684702 @default.
• W4224274981 cites W2785792383 @default.
• W4224274981 cites W2893838959 @default.
• W4224274981 cites W2973216672 @default.
• W4224274981 cites W3082632066 @default.
• W4224274981 cites W3134881099 @default.
• W4224274981 cites W3161432498 @default.
• W4224274981 cites W4234362248 @default.
• W4224274981 cites W4253785452 @default.
• W4224274981 doi "https://doi.org/10.1021/jacsau.2c00105" @default.
• W4224274981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35557753" @default.
• W4224274981 hasPublicationYear "2022" @default.
• W4224274981 type Work @default.
• W4224274981 citedByCount "4" @default.
• W4224274981 countsByYear W42242749812022 @default.
• W4224274981 countsByYear W42242749812023 @default.
• W4224274981 crossrefType "journal-article" @default.
• W4224274981 hasAuthorship W4224274981A5001520016 @default.
• W4224274981 hasAuthorship W4224274981A5004541105 @default.
• W4224274981 hasAuthorship W4224274981A5007102926 @default.
• W4224274981 hasAuthorship W4224274981A5075473900 @default.
• W4224274981 hasBestOaLocation W42242749811 @default.
• W4224274981 hasConcept C104317684 @default.
• W4224274981 hasConcept C107824862 @default.
• W4224274981 hasConcept C138885662 @default.
• W4224274981 hasConcept C153911025 @default.
• W4224274981 hasConcept C179926584 @default.
• W4224274981 hasConcept C2781320022 @default.
• W4224274981 hasConcept C41895202 @default.
• W4224274981 hasConcept C54355233 @default.
• W4224274981 hasConcept C552990157 @default.
• W4224274981 hasConcept C70721500 @default.
• W4224274981 hasConcept C86339819 @default.
• W4224274981 hasConcept C86803240 @default.
• W4224274981 hasConcept C95444343 @default.
• W4224274981 hasConceptScore W4224274981C104317684 @default.
• W4224274981 hasConceptScore W4224274981C107824862 @default.
• W4224274981 hasConceptScore W4224274981C138885662 @default.
• W4224274981 hasConceptScore W4224274981C153911025 @default.
• W4224274981 hasConceptScore W4224274981C179926584 @default.
• W4224274981 hasConceptScore W4224274981C2781320022 @default.
• W4224274981 hasConceptScore W4224274981C41895202 @default.
• W4224274981 hasConceptScore W4224274981C54355233 @default.
• W4224274981 hasConceptScore W4224274981C552990157 @default.
• W4224274981 hasConceptScore W4224274981C70721500 @default.
• W4224274981 hasConceptScore W4224274981C86339819 @default.
• W4224274981 hasConceptScore W4224274981C86803240 @default.
• W4224274981 hasConceptScore W4224274981C95444343 @default.
• W4224274981 hasFunder F4320319985 @default.
• W4224274981 hasFunder F4320334629 @default.
• W4224274981 hasIssue "4" @default.
• W4224274981 hasLocation W42242749811 @default.

• next