FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224277644> ?p ?o ?g. }

• W4224277644 abstract "Abstract Background miRNA-4701-5p has been reported to be a vital regulator in many diseases, including rheumatoid arthritis, and miRNA-4701-5p is evidenced to be participated in synovial invasion and joint destruction. In our report, we investigated the roles of miRNA-4701-5p in osteoarthritis (OA) and analyzed the molecular mechanism. Methods Interleukin-1β (IL-1β) was applied for stimulating human chondrocyte CHON-001 cells to establish an OA injury model. mRNA levels and protein expression were measured using qRT-PCR and western blot assay, respectively. The proliferation ability and cytotoxicity of CHON-001 cells were checked using MTT assay and lactate dehydrogenase activity. The inflammation of chondrocytes was accessed by the secretion levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor (TNF)-α. The apoptosis of chondrocytes was determined by flow cytometry assay. Bioinformatics software Starbase v2.0 analyzed the functional binding sites between miRNA-4701-5p and HMGA1 and the interaction was further confirmed using dual luciferase reporter analysis. Results: miRNA-4701-5p was down-regulated in the IL-1β-stimulated chondrocytes and HMGA1 directly targeted miRNA-4701-5p. Up-regulation of miRNA-4701-5p could alleviate IL-1β-treated CHON-001 cells inflammation and apoptosis, and reversed the cell proliferation decrease and cytotoxicity increase after IL-1β treatment. Nevertheless, all the roles of miRNA-4701-5p overexpression in CHON-001 cells could be reversed by HMGA1 up-regulation. Conclusions miRNA-4701-5p could alleviate the inflammatory injury of IL-1β-treated CHON-001 cells via down-regulating HMGA1, indicating that miRNA-4701-5p/HMGA1 is a promising therapeutic target for OA." @default.
• W4224277644 created "2022-04-26" @default.
• W4224277644 creator A5003759585 @default.
• W4224277644 creator A5006480146 @default.
• W4224277644 creator A5064548129 @default.
• W4224277644 date "2022-04-22" @default.
• W4224277644 modified "2023-09-25" @default.
• W4224277644 title "microRNA-4701-5p protects against interleukin-1β induced human chondrocyte CHON-001 cells injury via modulating HMGA1" @default.
• W4224277644 cites W1549438987 @default.
• W4224277644 cites W1971796262 @default.
• W4224277644 cites W1972204333 @default.
• W4224277644 cites W1990209489 @default.
• W4224277644 cites W2042851093 @default.
• W4224277644 cites W2046387002 @default.
• W4224277644 cites W2054891772 @default.
• W4224277644 cites W2072619434 @default.
• W4224277644 cites W2076131887 @default.
• W4224277644 cites W2085294880 @default.
• W4224277644 cites W2086781828 @default.
• W4224277644 cites W2100261712 @default.
• W4224277644 cites W2113424612 @default.
• W4224277644 cites W2133915381 @default.
• W4224277644 cites W2139853056 @default.
• W4224277644 cites W2146548807 @default.
• W4224277644 cites W2162506959 @default.
• W4224277644 cites W2163351331 @default.
• W4224277644 cites W2170342486 @default.
• W4224277644 cites W2325707043 @default.
• W4224277644 cites W2613036842 @default.
• W4224277644 cites W2620241325 @default.
• W4224277644 cites W2771085616 @default.
• W4224277644 cites W2771197141 @default.
• W4224277644 cites W2795291803 @default.
• W4224277644 cites W2807339315 @default.
• W4224277644 cites W2883019022 @default.
• W4224277644 cites W2904442971 @default.
• W4224277644 cites W2905684618 @default.
• W4224277644 cites W2908918388 @default.
• W4224277644 cites W2920832988 @default.
• W4224277644 cites W2928842550 @default.
• W4224277644 cites W2940368424 @default.
• W4224277644 cites W2944252756 @default.
• W4224277644 cites W2944845869 @default.
• W4224277644 cites W2963487203 @default.
• W4224277644 cites W2964413391 @default.
• W4224277644 cites W2979872515 @default.
• W4224277644 cites W2990857517 @default.
• W4224277644 cites W3013996114 @default.
• W4224277644 cites W3092209489 @default.
• W4224277644 cites W3123497340 @default.
• W4224277644 cites W4211069396 @default.
• W4224277644 cites W4235790631 @default.
• W4224277644 cites W4237076166 @default.
• W4224277644 doi "https://doi.org/10.1186/s13018-022-03083-8" @default.
• W4224277644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35459188" @default.
• W4224277644 hasPublicationYear "2022" @default.
• W4224277644 type Work @default.
• W4224277644 citedByCount "2" @default.
• W4224277644 countsByYear W42242776442023 @default.
• W4224277644 crossrefType "journal-article" @default.
• W4224277644 hasAuthorship W4224277644A5003759585 @default.
• W4224277644 hasAuthorship W4224277644A5006480146 @default.
• W4224277644 hasAuthorship W4224277644A5064548129 @default.
• W4224277644 hasBestOaLocation W42242776441 @default.
• W4224277644 hasConcept C104317684 @default.
• W4224277644 hasConcept C109316439 @default.
• W4224277644 hasConcept C127561419 @default.
• W4224277644 hasConcept C145059251 @default.
• W4224277644 hasConcept C153911025 @default.
• W4224277644 hasConcept C17991360 @default.
• W4224277644 hasConcept C185592680 @default.
• W4224277644 hasConcept C190283241 @default.
• W4224277644 hasConcept C202751555 @default.
• W4224277644 hasConcept C203014093 @default.
• W4224277644 hasConcept C2776914184 @default.
• W4224277644 hasConcept C2778690821 @default.
• W4224277644 hasConcept C502942594 @default.
• W4224277644 hasConcept C553184892 @default.
• W4224277644 hasConcept C55493867 @default.
• W4224277644 hasConcept C71924100 @default.
• W4224277644 hasConcept C74172505 @default.
• W4224277644 hasConcept C86803240 @default.
• W4224277644 hasConceptScore W4224277644C104317684 @default.
• W4224277644 hasConceptScore W4224277644C109316439 @default.
• W4224277644 hasConceptScore W4224277644C127561419 @default.
• W4224277644 hasConceptScore W4224277644C145059251 @default.
• W4224277644 hasConceptScore W4224277644C153911025 @default.
• W4224277644 hasConceptScore W4224277644C17991360 @default.
• W4224277644 hasConceptScore W4224277644C185592680 @default.
• W4224277644 hasConceptScore W4224277644C190283241 @default.
• W4224277644 hasConceptScore W4224277644C202751555 @default.
• W4224277644 hasConceptScore W4224277644C203014093 @default.
• W4224277644 hasConceptScore W4224277644C2776914184 @default.
• W4224277644 hasConceptScore W4224277644C2778690821 @default.
• W4224277644 hasConceptScore W4224277644C502942594 @default.
• W4224277644 hasConceptScore W4224277644C553184892 @default.
• W4224277644 hasConceptScore W4224277644C55493867 @default.
• W4224277644 hasConceptScore W4224277644C71924100 @default.
• W4224277644 hasConceptScore W4224277644C74172505 @default.
• W4224277644 hasConceptScore W4224277644C86803240 @default.

• next