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• W4224285004 startingPage "e0263102" @default.
• W4224285004 abstract "Glutamine binding protein (GlnBP) is an Escherichia Coli periplasmic binding protein, which binds and carries glutamine to the inner membrane ATP-binding cassette (ABC) transporter. GlnBP binds the ligand with affinity around 0.1μM measured by isothermal titration calorimetry (ITC) and ligand binding stabilizes protein structure shown by its increase in thermodynamic stability. However, the molecular determinant of GlnBP ligand binding is not known. Electrostatic and hydrophobic interaction between GlnBP and glutamine are critical factors. We propose that the freedome of closure movement is also vital for ligand binding. In order to approve this hypothesis, we generate a series of mutants with different linker length that has different magnitude of domain closure. Mutants show different ligand binding affinity, which indicates that the propensity of domain closure determines the ligand binding affinity. Ligand binding triggers gradual ensemble conformational change. Structural changes upon ligand binding are monitored by combination of small angle x-ray scattering (SAXS) and NMR spectroscopy. Detailed structure characterization of GlnBP contributes to a better understanding of ligand binding and provides the structural basis for biosensor design." @default.
• W4224285004 created "2022-04-26" @default.
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• W4224285004 date "2022-04-21" @default.
• W4224285004 modified "2023-10-05" @default.
• W4224285004 title "Reversible domain closure modulates GlnBP ligand binding affinity" @default.
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• W4224285004 doi "https://doi.org/10.1371/journal.pone.0263102" @default.
• W4224285004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35446849" @default.
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