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- W4224289156 abstract "Abstract Platelet-rich fibrin (PRF) is a hot research topic because of its regenerative effect in humans. However, data reporting about its application in companion animals is lacking . The aim of the study was to supplement currently available data on PRF cell composition in canine patients by isolating peripheral blood mononuclear cells (PBMC), namely T cells, matured B cells, monocytes and macrophages, and adapting current protocols of cell flow cytometry for PRF analysis. Canine patient population was divided into three subgroups: animals with periodontitis only, animals with neoplasia and periodontitis, and healthy controls. Individual clinical parameters of the patients and evaluation of the wound healing quality were included in the research. In the present study, canine PRF cell composition was analyzed for the first-time using cell flow cytometry protocol. A higher proportion of PBMC cells related to wound healing (CD3+, CD3+CD4+CD8-, CD14+) were found in the PRF of control, periodontitis and neoplasia groups compared to the respective blood samples which implies a positive outcome associated with clinical PRF usage in canine patients. Proportions of monocytes and macrophages were higher in PRF samples compared to blood of healthy patients and periodontitis affected patients, however, inflammatory and neoplastic processes have no effect on proportion distribution of PBMC." @default.
- W4224289156 created "2022-04-26" @default.
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- W4224289156 date "2022-04-22" @default.
- W4224289156 modified "2023-10-05" @default.
- W4224289156 title "Peripheral mononuclear cells composition in platelet-rich fibrin in canines with chronic conditions" @default.
- W4224289156 doi "https://doi.org/10.21203/rs.3.rs-1578452/v1" @default.
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