FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224770682> ?p ?o ?g. }

• W4224770682 abstract "Abstract RUN and FYVE domain-containing 3 (Rufy3) is a well-known adapter protein of a small GTPase protein family and is bound to the activated Ras family protein to maintain neuronal polarity. However, in experimental subarachnoid hemorrhage (SAH), the role of Rufy3 has not been investigated. Consequently, we aimed to investigate the potential role of Rufy3 in an in vivo model of SAH-induced early brain injury (EBI). In addition, we investigated the relevant brain-protective mechanisms. Oxyhemoglobin (OxyHb) stimulation of cultured primary neurons simulated vitro SAH condition. The SAH rat model was induced by infusing autologous blood into the optic chiasma pool and treating the rats with lentivirus-negative control 1 (LV-NC1), lentivirus-Rufy3 shRNA (LV-shRNA), lentivirus-negative control 2 (LV-NC2), lentivirus-Rufy3 (LV-Rufy3), or 8-pCPT-2′- O -Me-cAMP (8p-CPT) (Rap1 agonist). In experiment one, we found that the protein level of Rufy3 decreased and neuronal axon injury in the injured neurons but was rectified by LV-Rufy3 treatment. In experiment two, mRNA and protein levels of Rufy3 were downregulated in brain tissue and reached the lowest level at 24 h after SAH. In addition, the expression of Myelin Basic Protein was downregulated and that of anti-hypophosphorylated neurofilament H (N52) was upregulated after SAH. In experiments three and four, Rufy3 overexpression (LV-Rufy3) increased the interactions between Rufy3 and Rap1, the level of Rap1-GTP, and the ratio of Rap1-GTP/total GTP. In addition, LV-Rufy3 treatment inhibited axon injury and accelerated axon repair by activating the Rap1/Arap3/Rho/Fascin signaling pathway accompanied by upregulated protein expression levels of ARAP3, Rho, Fascin, and Facin. LV-Rufy3 also enhanced synaptic plasticity by activating the Rap1/MEK/ERK/synapsin I signaling pathway accompanied by upregulated protein expression levels of ERK1, p-ERK1, MEK1, p-MEK1, synaspin I, and p-synaspin I. Moreover, LV-Rufy3 also alleviated brain damage indicators, including cortical neuronal cell apoptosis and degeneration, brain edema, and cognitive impairment after SAH. However, the downregulation of Rufy3 had the opposite effect and aggravated EBI induced by SAH. Notably, the combined application of LV-Rufy3 and 8p-CPT showed a significant synergistic effect on the aforementioned parameters. Our findings suggest that enhanced Rufy3 expression may reduce EBI by inhibiting axon injury and promoting neuronal axon repair and synaptic plasticity after SAH." @default.
• W4224770682 created "2022-04-27" @default.
• W4224770682 creator A5003642180 @default.
• W4224770682 creator A5014741162 @default.
• W4224770682 creator A5041993686 @default.
• W4224770682 creator A5045067652 @default.
• W4224770682 creator A5057610627 @default.
• W4224770682 creator A5072809229 @default.
• W4224770682 creator A5075640940 @default.
• W4224770682 creator A5086495330 @default.
• W4224770682 date "2022-04-23" @default.
• W4224770682 modified "2023-10-17" @default.
• W4224770682 title "Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity" @default.
• W4224770682 cites W2000507870 @default.
• W4224770682 cites W2003659693 @default.
• W4224770682 cites W2021715667 @default.
• W4224770682 cites W2025259459 @default.
• W4224770682 cites W2065943378 @default.
• W4224770682 cites W2083600673 @default.
• W4224770682 cites W2085701948 @default.
• W4224770682 cites W2089986114 @default.
• W4224770682 cites W2090573528 @default.
• W4224770682 cites W2094051443 @default.
• W4224770682 cites W2110870557 @default.
• W4224770682 cites W2118947728 @default.
• W4224770682 cites W2151872315 @default.
• W4224770682 cites W2387584962 @default.
• W4224770682 cites W2501836428 @default.
• W4224770682 cites W2511685458 @default.
• W4224770682 cites W2519554346 @default.
• W4224770682 cites W2520731555 @default.
• W4224770682 cites W2587637916 @default.
• W4224770682 cites W2588686442 @default.
• W4224770682 cites W2621981548 @default.
• W4224770682 cites W2765214439 @default.
• W4224770682 cites W2796336382 @default.
• W4224770682 cites W2884246304 @default.
• W4224770682 cites W2892604754 @default.
• W4224770682 cites W2900537803 @default.
• W4224770682 cites W2903169869 @default.
• W4224770682 cites W2905799163 @default.
• W4224770682 cites W2913334492 @default.
• W4224770682 cites W2914769980 @default.
• W4224770682 cites W2934056354 @default.
• W4224770682 cites W2944038265 @default.
• W4224770682 cites W2944269146 @default.
• W4224770682 cites W2967445627 @default.
• W4224770682 cites W2970844985 @default.
• W4224770682 cites W2982644980 @default.
• W4224770682 cites W2997920261 @default.
• W4224770682 cites W3010653711 @default.
• W4224770682 cites W3024736971 @default.
• W4224770682 cites W3043084564 @default.
• W4224770682 cites W3045489293 @default.
• W4224770682 cites W3093216846 @default.
• W4224770682 cites W3109928581 @default.
• W4224770682 cites W3158209356 @default.
• W4224770682 doi "https://doi.org/10.1186/s13041-022-00919-6" @default.
• W4224770682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35461284" @default.
• W4224770682 hasPublicationYear "2022" @default.
• W4224770682 type Work @default.
• W4224770682 citedByCount "5" @default.
• W4224770682 countsByYear W42247706822023 @default.
• W4224770682 crossrefType "journal-article" @default.
• W4224770682 hasAuthorship W4224770682A5003642180 @default.
• W4224770682 hasAuthorship W4224770682A5014741162 @default.
• W4224770682 hasAuthorship W4224770682A5041993686 @default.
• W4224770682 hasAuthorship W4224770682A5045067652 @default.
• W4224770682 hasAuthorship W4224770682A5057610627 @default.
• W4224770682 hasAuthorship W4224770682A5072809229 @default.
• W4224770682 hasAuthorship W4224770682A5075640940 @default.
• W4224770682 hasAuthorship W4224770682A5086495330 @default.
• W4224770682 hasBestOaLocation W42247706821 @default.
• W4224770682 hasConcept C126322002 @default.
• W4224770682 hasConcept C134018914 @default.
• W4224770682 hasConcept C169760540 @default.
• W4224770682 hasConcept C2779530196 @default.
• W4224770682 hasConcept C42219234 @default.
• W4224770682 hasConcept C71924100 @default.
• W4224770682 hasConcept C86803240 @default.
• W4224770682 hasConceptScore W4224770682C126322002 @default.
• W4224770682 hasConceptScore W4224770682C134018914 @default.
• W4224770682 hasConceptScore W4224770682C169760540 @default.
• W4224770682 hasConceptScore W4224770682C2779530196 @default.
• W4224770682 hasConceptScore W4224770682C42219234 @default.
• W4224770682 hasConceptScore W4224770682C71924100 @default.
• W4224770682 hasConceptScore W4224770682C86803240 @default.
• W4224770682 hasFunder F4320321001 @default.
• W4224770682 hasFunder F4320322769 @default.
• W4224770682 hasIssue "1" @default.
• W4224770682 hasLocation W42247706821 @default.
• W4224770682 hasLocation W42247706822 @default.
• W4224770682 hasLocation W42247706823 @default.
• W4224770682 hasOpenAccess W4224770682 @default.
• W4224770682 hasPrimaryLocation W42247706821 @default.
• W4224770682 hasRelatedWork W1966504330 @default.
• W4224770682 hasRelatedWork W1966775726 @default.
• W4224770682 hasRelatedWork W1979139803 @default.
• W4224770682 hasRelatedWork W1991560548 @default.
• W4224770682 hasRelatedWork W2030889776 @default.

• next