FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4224797825> ?p ?o ?g. }

• W4224797825 abstract "μ-opioid receptors (MOR) are widely expressed in the brain, varying in density in different areas. Activation of MORs underlies analgesia, euphoria, but may lead to tolerance, dependence, and ultimately opioid addiction. The Purkinje cell (PC) is the only efferent neuron in the cerebellar cortex and receives glutamatergic synaptic inputs from the parallel fibers formed by the axons of granule cells. Studies have shown that MORs are expressed during the development of cerebellar cells. However, the distribution of MOR and their effects on PF-PC synaptic transmission remain unclear. To examine these questions, we used whole-cell patch clamp recordings and pharmacological methods to determine the effects and mechanisms of MOR activation on synaptic transmission at PF-PC synapses. The MOR-selective agonist DAMGO significantly reduced the amplitude and area under the curve (AUC) of PF-PC evoked (e) EPSCs, and increased the paired-pulse ratio (PPR).DAMGO-induced inhibitory effects on PF-PC eEPSCs and PPR were abolished by MOR specific blocker CTOP. Further, DAMGO significantly reduced the frequency of PF-PC mEPSCs, but had no obvious effect on their amplitude, suggesting a presynaptic site of action. The DAMGO-induced reduction in the frequency of PF-PC mEPSCs also was blocked by CTOP. A protein kinase A (PKA) inhibitor PKI added in the pipette solution did not affect the inhibitory effects on PF-PC mEPSCs induced by DAMGO. Both the PKA inhibitor K5720 and MEK inhibitor U0126 in artificial cerebrospinal fluid (ACSF) prevented the inhibitory effects of DAMGO on PF-PC mEPSCs. These findings reveal that MORs are expressed in presynaptic PF axon terminals, where DAMGO can activate presynaptic MORs to inhibit PF-PC synaptic transmission by regulating the release of glutamate. G-protein-dependent cAMP-PKA signaling pathway may be involved in this process." @default.
• W4224797825 created "2022-04-27" @default.
• W4224797825 creator A5004901078 @default.
• W4224797825 creator A5007042004 @default.
• W4224797825 creator A5014695294 @default.
• W4224797825 creator A5025217236 @default.
• W4224797825 creator A5044757881 @default.
• W4224797825 creator A5062647351 @default.
• W4224797825 creator A5063701979 @default.
• W4224797825 creator A5063908813 @default.
• W4224797825 date "2022-04-25" @default.
• W4224797825 modified "2023-10-10" @default.
• W4224797825 title "Mechanisms Underlying Mu Opioid Receptor Effects on Parallel Fiber-Purkinje Cell Synaptic Transmission in Mouse Cerebellar Cortex" @default.
• W4224797825 cites W1490490649 @default.
• W4224797825 cites W1960000138 @default.
• W4224797825 cites W1974999182 @default.
• W4224797825 cites W1978013478 @default.
• W4224797825 cites W1983204813 @default.
• W4224797825 cites W1987604639 @default.
• W4224797825 cites W1991377640 @default.
• W4224797825 cites W2023721269 @default.
• W4224797825 cites W2033470434 @default.
• W4224797825 cites W2036197192 @default.
• W4224797825 cites W2036694766 @default.
• W4224797825 cites W2058500729 @default.
• W4224797825 cites W2062586185 @default.
• W4224797825 cites W2062832052 @default.
• W4224797825 cites W2064009170 @default.
• W4224797825 cites W2094813942 @default.
• W4224797825 cites W2106438910 @default.
• W4224797825 cites W2107400659 @default.
• W4224797825 cites W2130449452 @default.
• W4224797825 cites W2133227617 @default.
• W4224797825 cites W2138694174 @default.
• W4224797825 cites W2153930945 @default.
• W4224797825 cites W2159100142 @default.
• W4224797825 cites W2159801302 @default.
• W4224797825 cites W2255693475 @default.
• W4224797825 cites W2302828574 @default.
• W4224797825 cites W2468767034 @default.
• W4224797825 cites W2478802193 @default.
• W4224797825 cites W2554642455 @default.
• W4224797825 cites W2565603421 @default.
• W4224797825 cites W2601465249 @default.
• W4224797825 cites W2626714978 @default.
• W4224797825 cites W2765689835 @default.
• W4224797825 cites W2794515548 @default.
• W4224797825 cites W2806568015 @default.
• W4224797825 cites W2903520456 @default.
• W4224797825 cites W2904761292 @default.
• W4224797825 cites W2918884175 @default.
• W4224797825 cites W2935642874 @default.
• W4224797825 cites W2943549269 @default.
• W4224797825 cites W2944411542 @default.
• W4224797825 cites W2969342955 @default.
• W4224797825 cites W2977986955 @default.
• W4224797825 cites W3016104954 @default.
• W4224797825 cites W3016218204 @default.
• W4224797825 cites W3034958011 @default.
• W4224797825 cites W3047152772 @default.
• W4224797825 cites W3110311047 @default.
• W4224797825 cites W3110964573 @default.
• W4224797825 cites W3122452343 @default.
• W4224797825 cites W4241152627 @default.
• W4224797825 doi "https://doi.org/10.3389/fnsyn.2022.862704" @default.
• W4224797825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35546898" @default.
• W4224797825 hasPublicationYear "2022" @default.
• W4224797825 type Work @default.
• W4224797825 citedByCount "2" @default.
• W4224797825 countsByYear W42247978252023 @default.
• W4224797825 crossrefType "journal-article" @default.
• W4224797825 hasAuthorship W4224797825A5004901078 @default.
• W4224797825 hasAuthorship W4224797825A5007042004 @default.
• W4224797825 hasAuthorship W4224797825A5014695294 @default.
• W4224797825 hasAuthorship W4224797825A5025217236 @default.
• W4224797825 hasAuthorship W4224797825A5044757881 @default.
• W4224797825 hasAuthorship W4224797825A5062647351 @default.
• W4224797825 hasAuthorship W4224797825A5063701979 @default.
• W4224797825 hasAuthorship W4224797825A5063908813 @default.
• W4224797825 hasBestOaLocation W42247978251 @default.
• W4224797825 hasConcept C112592302 @default.
• W4224797825 hasConcept C12554922 @default.
• W4224797825 hasConcept C14397066 @default.
• W4224797825 hasConcept C147944092 @default.
• W4224797825 hasConcept C169760540 @default.
• W4224797825 hasConcept C170493617 @default.
• W4224797825 hasConcept C17077164 @default.
• W4224797825 hasConcept C185263204 @default.
• W4224797825 hasConcept C185592680 @default.
• W4224797825 hasConcept C200170125 @default.
• W4224797825 hasConcept C2776401185 @default.
• W4224797825 hasConcept C2777803847 @default.
• W4224797825 hasConcept C2778938600 @default.
• W4224797825 hasConcept C2779652256 @default.
• W4224797825 hasConcept C2780648746 @default.
• W4224797825 hasConcept C2781063702 @default.
• W4224797825 hasConcept C2992333117 @default.
• W4224797825 hasConcept C55493867 @default.
• W4224797825 hasConcept C61174792 @default.
• W4224797825 hasConcept C86803240 @default.

• next