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• W4224883722 endingPage "102355" @default.
• W4224883722 startingPage "102355" @default.
• W4224883722 abstract "This work aimed to evaluate the ability of the isolate L. lactis LLH20 to synthesize bacteriocin-like inhibitory substances (BLIS) with antilisterial activity and its potential application for the biopreservation of minimally processed lettuce (Lactuca sativa L.). The BLIS showed antilisterial activity, sensitivity to proteolytic enzymes and stability at 100 °C, pH 2 to 10, chemical agents and temperatures of −20 °C for 105 days. The isolate LLH20 did not present positive results for lantibiotics related genes (lanB, lanC, lanM) and nisin, lacticin 481, lacticin 3147, and lactococcin 972. In 14 h of fermentation (MRS broth), the highest BLIS produced by LLH20 was identified, and the best condition found was a medium containing 1.25% glucose, pH 7 at 32 °C. The LLH20 has been shown to be safe for use in food by phenotypic tests. Lettuce was prepared, inoculated with Listeria monocytogenes (4.0 log CFU/g) and antimicrobial agents LLH20 (8.0 log CFU/g), nisin (2.5 ppm), BLIS of LLH20 (1600 AU/mL), or association of BLIS and nisin (1600 AU/mL and 2.5 ppm), and stored at 4 °C for 7 days. The L. monocytogenes counts in lettuce decreased by 3.14 and 1.63 log CFU/g, when inoculated with LLH20 or with BLIS from LLH20, respectively, being significantly different from the positive control (p < 0.05). The association of BLIS and nisin eliminated L. monocytogenes in minimally processed lettuce. These results indicate that L. lactis LLH20 synthesized a BLIS capable of controlling the growth of L. monocytogenes in lettuce stored at 4 °C, demonstrating its potential as a biopreservative." @default.
• W4224883722 created "2022-04-27" @default.
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• W4224883722 date "2022-07-01" @default.
• W4224883722 modified "2023-10-18" @default.
• W4224883722 title "Bacteriocin-like inhibitory substances (BLIS) synthesized by Lactococcus lactis LLH20: Antilisterial activity and application for biopreservation of minimally processed lettuce (Lactuca sativa L.)" @default.
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• W4224883722 doi "https://doi.org/10.1016/j.bcab.2022.102355" @default.
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