FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225094762> ?p ?o ?g. }

• W4225094762 abstract "Phenylpyruvate decarboxylase (PPDC) is a crucial enzyme that plays important roles in 2-phenylethanol (2-PE) biosynthesis. In our previous study, we screened a highly efficient PPDC KDC4427 from the novel 2-PE-producing strain Enterobacter sp. CGMCC 5087. Meanwhile, its decarboxylation activity of indolylpyruvate (IPyA) was also higher than other indolylpyruvate decarboxylases (IPDCs) reported so far. In this study, KDC4427 protein was purified and characterized, and its catalytic mechanisms were analyzed by biological methods. The optimum pH and temperature of KDC4427 was pH 6.5 and 35°C, respectively. The enzyme activity was relatively stable between pH 6 and 8 and over the range of temperatures from 25°C to 45°C. KDC4427 showed the highest catalytic efficiency on phenylpyruvic acid (PPA); meanwhile, it also showed high activity for IPyA and 2-ketobutanoic acid, and it was found that KDC4427 belongs to IPDCs by phylogenetic tree analysis. The coverage of the three-dimensional structure of KDC4427 and EcIPDC from Enterobacter cloacae was 96%. Leucine 542, one of the residues in the substrate-binding pocket, is replaced by isoleucine in KDC4427 compared with EcIPDC. Site-directed mutagenesis showed that the transition from leucine to isoleucine was unlikely to make KDC4427 have high catalytic activity for PPA and IPyA; the mutants at glutamate 468 almost completely lost catalytic activities for both PPA and IPyA, indicating that this glutamate was essential for the catalytic activity. Additionally, alanine 387 plays an important role in the substrate selectivity of KDC4427. IMPORTANCE Compared with the chemical synthesis of 2-phenylethanol (2-PE) by condensation of ethylene oxide and benzene, the biological synthesis of 2-PE is a potential method to replace the traditional process. This makes biotransformation gradually become the main way to produce high-quality 2-PE. Phenylpyruvate decarboxylase (PPDC) is the critical enzyme in 2-PE biosynthesis, and it is a momentous point of penetration to increase the production of 2-PE. In this regard, KDC4427 can catalyze phenylpyruvic acid (PPA) to phenylacetaldehyde more efficiently than any other PPDC previously reported. Moreover, it has high activity of indolepyruvate decarboxylases (IPDCs), which will be a great breakthrough in the synthesis of indole-3-acetic acid (IAA). With this study, we offer insights into the KDC4427 catalytic mechanism and significantly expand the toolbox of available α-ketoacid decarboxylases for application in biosynthesis." @default.
• W4225094762 created "2022-04-30" @default.
• W4225094762 creator A5004477087 @default.
• W4225094762 creator A5017296972 @default.
• W4225094762 creator A5048698429 @default.
• W4225094762 creator A5060911389 @default.
• W4225094762 creator A5066306425 @default.
• W4225094762 creator A5073808064 @default.
• W4225094762 date "2022-04-27" @default.
• W4225094762 modified "2023-10-01" @default.
• W4225094762 title "The Characterization of an Efficient Phenylpyruvate Decarboxylase KDC4427, Involved in 2-Phenylethanol and IAA Production from Bacterial Enterobacter sp. CGMCC 5087" @default.
• W4225094762 cites W1562095280 @default.
• W4225094762 cites W1579120832 @default.
• W4225094762 cites W1600317475 @default.
• W4225094762 cites W1608001440 @default.
• W4225094762 cites W1864838007 @default.
• W4225094762 cites W1978119727 @default.
• W4225094762 cites W1983268283 @default.
• W4225094762 cites W1985744610 @default.
• W4225094762 cites W2000046950 @default.
• W4225094762 cites W2003013017 @default.
• W4225094762 cites W2004061485 @default.
• W4225094762 cites W2004649743 @default.
• W4225094762 cites W2010046474 @default.
• W4225094762 cites W2010571488 @default.
• W4225094762 cites W2031856032 @default.
• W4225094762 cites W2038554141 @default.
• W4225094762 cites W2044997975 @default.
• W4225094762 cites W2051934653 @default.
• W4225094762 cites W2065059911 @default.
• W4225094762 cites W2068490496 @default.
• W4225094762 cites W2084757892 @default.
• W4225094762 cites W2106471781 @default.
• W4225094762 cites W2138041440 @default.
• W4225094762 cites W2145092942 @default.
• W4225094762 cites W2145801816 @default.
• W4225094762 cites W2174314737 @default.
• W4225094762 cites W2330898901 @default.
• W4225094762 cites W2600786160 @default.
• W4225094762 cites W2737844151 @default.
• W4225094762 cites W2792905725 @default.
• W4225094762 cites W2899535034 @default.
• W4225094762 cites W2905674682 @default.
• W4225094762 cites W2912405537 @default.
• W4225094762 cites W2915627658 @default.
• W4225094762 cites W3040357278 @default.
• W4225094762 doi "https://doi.org/10.1128/spectrum.02660-21" @default.
• W4225094762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35377224" @default.
• W4225094762 hasPublicationYear "2022" @default.
• W4225094762 type Work @default.
• W4225094762 citedByCount "1" @default.
• W4225094762 countsByYear W42250947622023 @default.
• W4225094762 crossrefType "journal-article" @default.
• W4225094762 hasAuthorship W4225094762A5004477087 @default.
• W4225094762 hasAuthorship W4225094762A5017296972 @default.
• W4225094762 hasAuthorship W4225094762A5048698429 @default.
• W4225094762 hasAuthorship W4225094762A5060911389 @default.
• W4225094762 hasAuthorship W4225094762A5066306425 @default.
• W4225094762 hasAuthorship W4225094762A5073808064 @default.
• W4225094762 hasBestOaLocation W42250947623 @default.
• W4225094762 hasConcept C104317684 @default.
• W4225094762 hasConcept C161790260 @default.
• W4225094762 hasConcept C181199279 @default.
• W4225094762 hasConcept C185592680 @default.
• W4225094762 hasConcept C18903297 @default.
• W4225094762 hasConcept C2776580952 @default.
• W4225094762 hasConcept C2777289219 @default.
• W4225094762 hasConcept C2777305461 @default.
• W4225094762 hasConcept C2777402170 @default.
• W4225094762 hasConcept C2778910516 @default.
• W4225094762 hasConcept C2778983983 @default.
• W4225094762 hasConcept C2779856020 @default.
• W4225094762 hasConcept C2780595602 @default.
• W4225094762 hasConcept C41183919 @default.
• W4225094762 hasConcept C515207424 @default.
• W4225094762 hasConcept C547475151 @default.
• W4225094762 hasConcept C55493867 @default.
• W4225094762 hasConcept C71240020 @default.
• W4225094762 hasConcept C86803240 @default.
• W4225094762 hasConceptScore W4225094762C104317684 @default.
• W4225094762 hasConceptScore W4225094762C161790260 @default.
• W4225094762 hasConceptScore W4225094762C181199279 @default.
• W4225094762 hasConceptScore W4225094762C185592680 @default.
• W4225094762 hasConceptScore W4225094762C18903297 @default.
• W4225094762 hasConceptScore W4225094762C2776580952 @default.
• W4225094762 hasConceptScore W4225094762C2777289219 @default.
• W4225094762 hasConceptScore W4225094762C2777305461 @default.
• W4225094762 hasConceptScore W4225094762C2777402170 @default.
• W4225094762 hasConceptScore W4225094762C2778910516 @default.
• W4225094762 hasConceptScore W4225094762C2778983983 @default.
• W4225094762 hasConceptScore W4225094762C2779856020 @default.
• W4225094762 hasConceptScore W4225094762C2780595602 @default.
• W4225094762 hasConceptScore W4225094762C41183919 @default.
• W4225094762 hasConceptScore W4225094762C515207424 @default.
• W4225094762 hasConceptScore W4225094762C547475151 @default.
• W4225094762 hasConceptScore W4225094762C55493867 @default.
• W4225094762 hasConceptScore W4225094762C71240020 @default.
• W4225094762 hasConceptScore W4225094762C86803240 @default.
• W4225094762 hasIssue "2" @default.
• W4225094762 hasLocation W42250947621 @default.

• next