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- W4225105874 abstract "Abstract Background: Lumpy skin virus causes huge economic losses in farm animals. Herpesviridae are opportunistic and showed synergistic role but worsen disease symptoms. By using TEM & negative staining one avoid the problematic concerns of differential diagnosis.Materials and Methods: Cutaneous crusts collected from infected cattle, samples examined directly by the electron microscopy, then confirmed by the Conventional Polymerase Chain Reaction and partially sequenced by ORF103 gene.Results: This study reveals that the source of infection was not related to the vaccines. It turns out to be a natural infection by Lumpy skin disease virus isolate Evros / GR/15 and 99% identity. A comparison of the gene sequence of the vaccine against the virus showed significant divergence between them. Negative staining electron microscopy reveals Lumpy skin disease virus & Herpes viruses in different sizes and forms. Herpesviridae members were contaminating LSD lesions as TEM reveals the presence of different herpes types. Conclusions: LSD virus of cattle showed virulent nature in natural outbreak. However, the herpes viruses invade infected tissues and propagate inside cells of lumps that revealed its opportunistic behavior. Consequently; Antivirals against Herpesviridae would theoretically relief lumpy skin disease symptoms and help in control efforts." @default.
- W4225105874 created "2022-04-30" @default.
- W4225105874 creator A5060265879 @default.
- W4225105874 date "2022-01-28" @default.
- W4225105874 modified "2023-09-25" @default.
- W4225105874 title "Lumpy Skin Virus; Herpesviridae Inevitable Synergism" @default.
- W4225105874 doi "https://doi.org/10.21203/rs.3.rs-1290778/v1" @default.
- W4225105874 hasPublicationYear "2022" @default.
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