FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225134889> ?p ?o ?g. }

• W4225134889 endingPage "e00199" @default.
• W4225134889 startingPage "e00199" @default.
• W4225134889 abstract "The fully biobased polyhydroxyalkanoate (PHA) polymers provide interesting alternatives for petrochemical derived plastic materials. The mechanical properties of some PHAs, including the common poly(3-hydroxybutyrate) (PHB), are limited, but tunable by addition of other monomers into the polymer chain. In this study we present a precise synthetic biology method to adjust lactate monomer fraction of a polymer by controlling the monomer formation in vivo at gene expression level, independent of cultivation conditions. We used the modified doxycycline-based Tet-On approach to adjust the expression of the stereospecific D-lactate dehydrogenase gene (ldhA) from Leuconostoc mesenteroides to control D-lactic acid formation in yeast Saccharomyces cerevisiae. The synthetic Tet-On transcription factor with a VP16 activation domain was continuously expressed and its binding to a synthetic promoter with eight transcription factor specific binding sites upstream of the ldhA gene was controlled with the doxycycline concentration in the media. The increase in doxycycline concentration correlated positively with ldhA expression, D-lactic acid production, poly(D-lactic acid) (PDLA) accumulation in vivo, and D-lactic acid content in the poly(D-lactate-co-3-hydroxybutyrate) P(LA-3HB) copolymer. We demonstrated that the D-lactic acid content of the P(LA-3HB) copolymer can be adjusted linearly from 6 mol% to 93 mol% in vivo in S. cerevisiae. These results highlight the power of controlling gene expression and monomer formation in the tuning of the polymer composition. In addition, we obtained 5.6% PDLA and 19% P(LA-3HB) of the cell dry weight (CDW), which are over two- and five-fold higher accumulation levels, respectively, than reported in the previous studies with yeast. We also compared two engineered PHA synthases and discovered that in S. cerevisiae the PHA synthase PhaC1437Ps6-19 produced P(LA-3HB) copolymers with lower D-lactic acid content, but with higher molecular weight, in comparison to the PHA synthase PhaC1Pre." @default.
• W4225134889 created "2022-05-01" @default.
• W4225134889 creator A5037173840 @default.
• W4225134889 creator A5043243404 @default.
• W4225134889 creator A5063624676 @default.
• W4225134889 creator A5077148932 @default.
• W4225134889 date "2022-06-01" @default.
• W4225134889 modified "2023-09-25" @default.
• W4225134889 title "Control of D-lactic acid content in P(LA-3HB) copolymer in the yeast Saccharomyces cerevisiae using a synthetic gene expression system" @default.
• W4225134889 cites W1529218396 @default.
• W4225134889 cites W1823107592 @default.
• W4225134889 cites W1909944883 @default.
• W4225134889 cites W1969210384 @default.
• W4225134889 cites W1970875205 @default.
• W4225134889 cites W1980729056 @default.
• W4225134889 cites W1984158735 @default.
• W4225134889 cites W1985241293 @default.
• W4225134889 cites W1995873602 @default.
• W4225134889 cites W1996745037 @default.
• W4225134889 cites W1998428522 @default.
• W4225134889 cites W2001876121 @default.
• W4225134889 cites W2004694970 @default.
• W4225134889 cites W2005275397 @default.
• W4225134889 cites W2017224043 @default.
• W4225134889 cites W2019611830 @default.
• W4225134889 cites W2022751806 @default.
• W4225134889 cites W2024763584 @default.
• W4225134889 cites W2028548623 @default.
• W4225134889 cites W2038591876 @default.
• W4225134889 cites W2048285039 @default.
• W4225134889 cites W2098113421 @default.
• W4225134889 cites W2103345065 @default.
• W4225134889 cites W2109045184 @default.
• W4225134889 cites W2115774649 @default.
• W4225134889 cites W2123794239 @default.
• W4225134889 cites W2135022383 @default.
• W4225134889 cites W2156252266 @default.
• W4225134889 cites W2156780012 @default.
• W4225134889 cites W2157429392 @default.
• W4225134889 cites W2160698526 @default.
• W4225134889 cites W2163649565 @default.
• W4225134889 cites W2167149191 @default.
• W4225134889 cites W2276111297 @default.
• W4225134889 cites W2282698399 @default.
• W4225134889 cites W2313393860 @default.
• W4225134889 cites W2347725975 @default.
• W4225134889 cites W2398436462 @default.
• W4225134889 cites W2468401125 @default.
• W4225134889 cites W2469269688 @default.
• W4225134889 cites W2554275288 @default.
• W4225134889 cites W2587257719 @default.
• W4225134889 cites W2595391269 @default.
• W4225134889 cites W2747258567 @default.
• W4225134889 cites W2765706804 @default.
• W4225134889 cites W2800638982 @default.
• W4225134889 cites W2800997817 @default.
• W4225134889 cites W2809251183 @default.
• W4225134889 cites W2911548538 @default.
• W4225134889 cites W2947336536 @default.
• W4225134889 cites W2947949221 @default.
• W4225134889 cites W3007679734 @default.
• W4225134889 cites W3093569559 @default.
• W4225134889 cites W3111638286 @default.
• W4225134889 cites W3158489638 @default.
• W4225134889 cites W753938694 @default.
• W4225134889 doi "https://doi.org/10.1016/j.mec.2022.e00199" @default.
• W4225134889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35571351" @default.
• W4225134889 hasPublicationYear "2022" @default.
• W4225134889 type Work @default.
• W4225134889 citedByCount "0" @default.
• W4225134889 crossrefType "journal-article" @default.
• W4225134889 hasAuthorship W4225134889A5037173840 @default.
• W4225134889 hasAuthorship W4225134889A5043243404 @default.
• W4225134889 hasAuthorship W4225134889A5063624676 @default.
• W4225134889 hasAuthorship W4225134889A5077148932 @default.
• W4225134889 hasBestOaLocation W42251348891 @default.
• W4225134889 hasConcept C104317684 @default.
• W4225134889 hasConcept C150194340 @default.
• W4225134889 hasConcept C185592680 @default.
• W4225134889 hasConcept C2775920511 @default.
• W4225134889 hasConcept C2777576037 @default.
• W4225134889 hasConcept C2779222958 @default.
• W4225134889 hasConcept C523546767 @default.
• W4225134889 hasConcept C54355233 @default.
• W4225134889 hasConcept C55493867 @default.
• W4225134889 hasConcept C73447357 @default.
• W4225134889 hasConcept C86803240 @default.
• W4225134889 hasConceptScore W4225134889C104317684 @default.
• W4225134889 hasConceptScore W4225134889C150194340 @default.
• W4225134889 hasConceptScore W4225134889C185592680 @default.
• W4225134889 hasConceptScore W4225134889C2775920511 @default.
• W4225134889 hasConceptScore W4225134889C2777576037 @default.
• W4225134889 hasConceptScore W4225134889C2779222958 @default.
• W4225134889 hasConceptScore W4225134889C523546767 @default.
• W4225134889 hasConceptScore W4225134889C54355233 @default.
• W4225134889 hasConceptScore W4225134889C55493867 @default.
• W4225134889 hasConceptScore W4225134889C73447357 @default.
• W4225134889 hasConceptScore W4225134889C86803240 @default.

• next