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- W4225163853 endingPage "459" @default.
- W4225163853 startingPage "459" @default.
- W4225163853 abstract "Botrytis virus F (BVF) is a positive-sense, single-stranded RNA (+ssRNA) virus within the Gammaflexiviridae family of the plant-pathogenic fungus Botrytis cinerea. In this study, the complete sequence of a BVF strain isolated from B. cinerea collected from grapevine fields in Spain was analyzed. This virus, in this work BVF-V448, has a genome of 6827 nt in length, excluding the poly(A) tail, with two open reading frames encoding an RNA dependent RNA polymerase (RdRP) and a coat protein (CP). The 5'- and 3'-terminal regions of the genome were determined by rapid amplification of cDNA ends (RACE). Furthermore, a yet undetected subgenomic RNA species in BVF-V448 was identified, indicating that the CP is expressed via 3' coterminal subgenomic RNAs (sgRNAs). We also report the successful construction of the first BVF full-length cDNA clone and synthesized in vitro RNA transcripts using the T7 polymerase, which could efficiently transfect two different strains of B. cinerea, B05.10 and Pi258.9. The levels of growth in culture and virulence on plants of BVF-V448 transfected strains were comparable to BVF-free strains. The infectious clones generated in this work provide a useful tool for the future development of an efficient BVF foreign gene expression vector and a virus-induced gene silencing (VIGS) vector as a biological agent for the control of B. cinerea." @default.
- W4225163853 created "2022-05-01" @default.
- W4225163853 creator A5001562642 @default.
- W4225163853 creator A5008467703 @default.
- W4225163853 creator A5016627043 @default.
- W4225163853 creator A5061717913 @default.
- W4225163853 date "2022-04-29" @default.
- W4225163853 modified "2023-10-02" @default.
- W4225163853 title "Construction and Characterization of a Botrytis Virus F Infectious Clone" @default.
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- W4225163853 doi "https://doi.org/10.3390/jof8050459" @default.
- W4225163853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35628716" @default.
- W4225163853 hasPublicationYear "2022" @default.
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