Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225163970> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W4225163970 endingPage "101612" @default.
- W4225163970 startingPage "101612" @default.
- W4225163970 abstract "Spinal cord injury (SCI) is one of the serious neurological diseases with high morbidity which may be treated with hematopoietic stem cell (HSC) transplants. Circular RNAs (circRNAs) play vital roles in SCI. The study aimed to reveal the function and mechanism of circRNA homeodomain interacting protein kinase 3 (HIPK3) in SCI.SCI model in vitro was established by treating neuronal cells AGE1.HN with oxygen-glucose deprivation (OGD) and CoCl2. The levels of circHIPK3, miR-382-5p and dual specificity phosphatase 1 (DUSP1) were examined using quantitative real-time PCR (qRT-PCR) or western blot assay. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory factors (IL-6 and TNF-α). Cell proliferation and apoptosis were evaluated by 5'-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry. Caspase-3 Colorimetric Assay Kit was used to detect aaspase-3 activity. The interactions among circHIPK3, miR-382-5p and DUSP1 were confirmed by dual-luciferase reporter and RNA immunoprecipitation assays.CircHIPK3 and DUSP1 were down-regulated, while miR-382-5p was up-regulated in OGD-induced AGE1.HN cells. Overexpression of circHIPK3 suppressed inflammatory response and cell apoptosis and promoted proliferation in OGD-induced AGE1.HN cells by sponging miR-382-5p. CircHIPK3 regulated DUSP1 expression by targeting miR-382-5p. MiR-382-5p inhibition hindered inflammatory response of IL-6 and TNF-α and neuronal apoptosis and promoted apoptosis via targeting DUSP1.CircHIPK3 overexpression alleviated OGD-induced AGE1.HN cell inflammatory response and neuronal apoptosis via regulating miR-382-5p/DUSP1 axis, indicating that circHIPK3 might be a promising therapeutic target for SCI." @default.
- W4225163970 created "2022-05-01" @default.
- W4225163970 creator A5007472721 @default.
- W4225163970 creator A5030977325 @default.
- W4225163970 creator A5054821477 @default.
- W4225163970 creator A5068929021 @default.
- W4225163970 creator A5074225347 @default.
- W4225163970 creator A5085629666 @default.
- W4225163970 date "2022-08-01" @default.
- W4225163970 modified "2023-10-15" @default.
- W4225163970 title "CircHIPK3 alleviates inflammatory response and neuronal apoptosis via regulating miR-382-5p/DUSP1 axis in spinal cord injury" @default.
- W4225163970 cites W2140844751 @default.
- W4225163970 cites W2203373231 @default.
- W4225163970 cites W2608611322 @default.
- W4225163970 cites W2727459662 @default.
- W4225163970 cites W2770429776 @default.
- W4225163970 cites W2782414823 @default.
- W4225163970 cites W2795917193 @default.
- W4225163970 cites W2802810292 @default.
- W4225163970 cites W2883062882 @default.
- W4225163970 cites W2891075973 @default.
- W4225163970 cites W2916004720 @default.
- W4225163970 cites W2949078846 @default.
- W4225163970 cites W2966433496 @default.
- W4225163970 cites W2990414011 @default.
- W4225163970 cites W2991061620 @default.
- W4225163970 cites W3006314414 @default.
- W4225163970 cites W3014138137 @default.
- W4225163970 cites W3021141767 @default.
- W4225163970 cites W3025087299 @default.
- W4225163970 cites W3033463350 @default.
- W4225163970 cites W3081390264 @default.
- W4225163970 cites W3091771919 @default.
- W4225163970 cites W3100340233 @default.
- W4225163970 cites W3115422121 @default.
- W4225163970 cites W3119966594 @default.
- W4225163970 cites W3137552168 @default.
- W4225163970 cites W3139620078 @default.
- W4225163970 cites W3186495872 @default.
- W4225163970 cites W3188559406 @default.
- W4225163970 cites W3191303725 @default.
- W4225163970 doi "https://doi.org/10.1016/j.trim.2022.101612" @default.
- W4225163970 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35500847" @default.
- W4225163970 hasPublicationYear "2022" @default.
- W4225163970 type Work @default.
- W4225163970 citedByCount "5" @default.
- W4225163970 countsByYear W42251639702022 @default.
- W4225163970 countsByYear W42251639702023 @default.
- W4225163970 crossrefType "journal-article" @default.
- W4225163970 hasAuthorship W4225163970A5007472721 @default.
- W4225163970 hasAuthorship W4225163970A5030977325 @default.
- W4225163970 hasAuthorship W4225163970A5054821477 @default.
- W4225163970 hasAuthorship W4225163970A5068929021 @default.
- W4225163970 hasAuthorship W4225163970A5074225347 @default.
- W4225163970 hasAuthorship W4225163970A5085629666 @default.
- W4225163970 hasConcept C104317684 @default.
- W4225163970 hasConcept C153911025 @default.
- W4225163970 hasConcept C185592680 @default.
- W4225163970 hasConcept C190283241 @default.
- W4225163970 hasConcept C2776415932 @default.
- W4225163970 hasConcept C502942594 @default.
- W4225163970 hasConcept C553184892 @default.
- W4225163970 hasConcept C55493867 @default.
- W4225163970 hasConcept C86803240 @default.
- W4225163970 hasConcept C95444343 @default.
- W4225163970 hasConceptScore W4225163970C104317684 @default.
- W4225163970 hasConceptScore W4225163970C153911025 @default.
- W4225163970 hasConceptScore W4225163970C185592680 @default.
- W4225163970 hasConceptScore W4225163970C190283241 @default.
- W4225163970 hasConceptScore W4225163970C2776415932 @default.
- W4225163970 hasConceptScore W4225163970C502942594 @default.
- W4225163970 hasConceptScore W4225163970C553184892 @default.
- W4225163970 hasConceptScore W4225163970C55493867 @default.
- W4225163970 hasConceptScore W4225163970C86803240 @default.
- W4225163970 hasConceptScore W4225163970C95444343 @default.
- W4225163970 hasLocation W42251639701 @default.
- W4225163970 hasLocation W42251639702 @default.
- W4225163970 hasOpenAccess W4225163970 @default.
- W4225163970 hasPrimaryLocation W42251639701 @default.
- W4225163970 hasRelatedWork W2041067052 @default.
- W4225163970 hasRelatedWork W2253642286 @default.
- W4225163970 hasRelatedWork W2351699250 @default.
- W4225163970 hasRelatedWork W2363178760 @default.
- W4225163970 hasRelatedWork W2387700717 @default.
- W4225163970 hasRelatedWork W2390313530 @default.
- W4225163970 hasRelatedWork W2390532320 @default.
- W4225163970 hasRelatedWork W2392942585 @default.
- W4225163970 hasRelatedWork W3000092425 @default.
- W4225163970 hasRelatedWork W3032579496 @default.
- W4225163970 hasVolume "73" @default.
- W4225163970 isParatext "false" @default.
- W4225163970 isRetracted "false" @default.
- W4225163970 workType "article" @default.