FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225258946> ?p ?o ?g. }

• W4225258946 abstract "You have accessJournal of UrologyCME1 May 2022PD48-10 TESTICULAR TRANSCRIPTOME ANALYSIS REVEALS POTENTIAL GENES ASSOCIATED WITH ENDOCRINE SYSTEM IN A MOUSE MODEL OF COMPENSATED TESTICULAR HYPERTROPHY Daniel E. Nassau, Alexandra Dullea, Eliyahu Kresch, Vinayak Madhusoodanan, Shathiyah Kulandavelu, Miguel Castellan, Himanshu Arora, and Ranjith Ramasamy Daniel E. NassauDaniel E. Nassau More articles by this author , Alexandra DulleaAlexandra Dullea More articles by this author , Eliyahu KreschEliyahu Kresch More articles by this author , Vinayak MadhusoodananVinayak Madhusoodanan More articles by this author , Shathiyah KulandaveluShathiyah Kulandavelu More articles by this author , Miguel CastellanMiguel Castellan More articles by this author , Himanshu AroraHimanshu Arora More articles by this author , and Ranjith RamasamyRanjith Ramasamy More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002616.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Animal models demonstrate compensatory growth after unilateral orchiectomy when loss occurs before puberty, but absent if performed post-pubertally. The mechanisms leading to testicular hypertrophy after tissue loss remain unknown. We hypothesized that cellular pathways involving somatic growth and reproduction would be upregulated in the prepubertal groups compared to control. We performed RNA sequencing (RNASeq) on the contralateral testis after hemi-castration was performed at different ages in C57BL/6 mice. METHODS: An abdominal approach unilateral orchiectomy was performed in C57BL/6 mice at either the neonatal, prepubertal or post-pubertal periods between days of life (DOL) 2-4, 20-22 and 42-44, respectively. Experimental mice and controls were sacrificed after reaching physical maturity (DOL 80). The remaining testis was weighed and underwent RNASeq. The RNA library was generated and protein coding genes with p<0.05, FDR<0.05 were subjected to enrichment using Ingenuity pathway analysis (IPA). Expression of the candidate genes was evaluated using two-way ANOVA, allowing the identification of significantly different expression within the experimental groups compared to the control. RESULTS: Mean testis weight in each group (n=3) was 110.6mg (Neonate), 104.7mg (Prepubertal), 101.2 (Post-pubertal) and 85.5 (control) indicating larger testis on the contralateral side as an adult if orchiectomy was at an earlier age. IPA showed 502 different pathways/functions with which candidate genes were involved. A total of 58 unique protein coding genes met the criteria for analysis. Of these, Tubulin Tyrosine Ligase Like 2 (Ttll2) and Sacrosine Dehydrogenase (SARDH) had significantly increased, and decreased expression compared with controls, respectively (Figure). Ttll2 and SARDH are enriched in pathways such as endocrine system disorder, reproductive system disease, developmental disorders and metabolic diseases. CONCLUSIONS: We identified two genes involved in endocrine pathways that likely have altered expression in response to hemi-castration. Further work is needed to understand how these genes may be involved in testicular growth, and how pre-pubertal testis loss influences hypertrophy as compared to loss of testis as an adult. Source of Funding: This work was supported by National Institutes of Health Grant R01 DK130991, and Clinician Scientist Development Grant from the American Cancer Society to Ranjith Ramasamy © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e803 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Daniel E. Nassau More articles by this author Alexandra Dullea More articles by this author Eliyahu Kresch More articles by this author Vinayak Madhusoodanan More articles by this author Shathiyah Kulandavelu More articles by this author Miguel Castellan More articles by this author Himanshu Arora More articles by this author Ranjith Ramasamy More articles by this author Expand All Advertisement PDF DownloadLoading ..." @default.
• W4225258946 created "2022-05-04" @default.
• W4225258946 creator A5025064669 @default.
• W4225258946 creator A5025348800 @default.
• W4225258946 creator A5037103639 @default.
• W4225258946 creator A5046165296 @default.
• W4225258946 creator A5058868383 @default.
• W4225258946 creator A5071253043 @default.
• W4225258946 creator A5073833314 @default.
• W4225258946 creator A5082062645 @default.
• W4225258946 date "2022-05-01" @default.
• W4225258946 modified "2023-09-27" @default.
• W4225258946 title "PD48-10 TESTICULAR TRANSCRIPTOME ANALYSIS REVEALS POTENTIAL GENES ASSOCIATED WITH ENDOCRINE SYSTEM IN A MOUSE MODEL OF COMPENSATED TESTICULAR HYPERTROPHY" @default.
• W4225258946 doi "https://doi.org/10.1097/ju.0000000000002616.10" @default.
• W4225258946 hasPublicationYear "2022" @default.
• W4225258946 type Work @default.
• W4225258946 citedByCount "0" @default.
• W4225258946 crossrefType "journal-article" @default.
• W4225258946 hasAuthorship W4225258946A5025064669 @default.
• W4225258946 hasAuthorship W4225258946A5025348800 @default.
• W4225258946 hasAuthorship W4225258946A5037103639 @default.
• W4225258946 hasAuthorship W4225258946A5046165296 @default.
• W4225258946 hasAuthorship W4225258946A5058868383 @default.
• W4225258946 hasAuthorship W4225258946A5071253043 @default.
• W4225258946 hasAuthorship W4225258946A5073833314 @default.
• W4225258946 hasAuthorship W4225258946A5082062645 @default.
• W4225258946 hasConcept C104317684 @default.
• W4225258946 hasConcept C126322002 @default.
• W4225258946 hasConcept C127561419 @default.
• W4225258946 hasConcept C134018914 @default.
• W4225258946 hasConcept C134305767 @default.
• W4225258946 hasConcept C150194340 @default.
• W4225258946 hasConcept C162317418 @default.
• W4225258946 hasConcept C16685009 @default.
• W4225258946 hasConcept C167414201 @default.
• W4225258946 hasConcept C2781440318 @default.
• W4225258946 hasConcept C46699223 @default.
• W4225258946 hasConcept C54355233 @default.
• W4225258946 hasConcept C71315377 @default.
• W4225258946 hasConcept C71924100 @default.
• W4225258946 hasConcept C86803240 @default.
• W4225258946 hasConceptScore W4225258946C104317684 @default.
• W4225258946 hasConceptScore W4225258946C126322002 @default.
• W4225258946 hasConceptScore W4225258946C127561419 @default.
• W4225258946 hasConceptScore W4225258946C134018914 @default.
• W4225258946 hasConceptScore W4225258946C134305767 @default.
• W4225258946 hasConceptScore W4225258946C150194340 @default.
• W4225258946 hasConceptScore W4225258946C162317418 @default.
• W4225258946 hasConceptScore W4225258946C16685009 @default.
• W4225258946 hasConceptScore W4225258946C167414201 @default.
• W4225258946 hasConceptScore W4225258946C2781440318 @default.
• W4225258946 hasConceptScore W4225258946C46699223 @default.
• W4225258946 hasConceptScore W4225258946C54355233 @default.
• W4225258946 hasConceptScore W4225258946C71315377 @default.
• W4225258946 hasConceptScore W4225258946C71924100 @default.
• W4225258946 hasConceptScore W4225258946C86803240 @default.
• W4225258946 hasIssue "Supplement 5" @default.
• W4225258946 hasLocation W42252589461 @default.
• W4225258946 hasOpenAccess W4225258946 @default.
• W4225258946 hasPrimaryLocation W42252589461 @default.
• W4225258946 hasRelatedWork W1514902666 @default.
• W4225258946 hasRelatedWork W1987866665 @default.
• W4225258946 hasRelatedWork W2020449916 @default.
• W4225258946 hasRelatedWork W2025703417 @default.
• W4225258946 hasRelatedWork W2091504832 @default.
• W4225258946 hasRelatedWork W2107729314 @default.
• W4225258946 hasRelatedWork W2157270415 @default.
• W4225258946 hasRelatedWork W2409608972 @default.
• W4225258946 hasRelatedWork W4220896095 @default.
• W4225258946 hasRelatedWork W4233650373 @default.
• W4225258946 hasVolume "207" @default.
• W4225258946 isParatext "false" @default.
• W4225258946 isRetracted "false" @default.
• W4225258946 workType "article" @default.